Characterization of Mixed-Species Biofilm Formed by Vibrio parahaemolyticus and Listeria monocytogenes
Mixed-species biofilms are the predominant form of biofilms found in nature. Research on biofilms have typically concentrated on single species biofilms and this study expands the horizon of biofilm research, where the characterization and dynamic changes of mono and mixed-species biofilms formed by...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02543/full |
| _version_ | 1818516742382551040 |
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| author | Ping Chen Jing Jing Wang Jing Jing Wang Jing Jing Wang Bin Hong Ling Tan Jun Yan Jun Yan Jun Yan Zhaohuan Zhang Haiquan Liu Haiquan Liu Haiquan Liu Haiquan Liu Yingjie Pan Yingjie Pan Yingjie Pan Yong Zhao Yong Zhao Yong Zhao |
| author_facet | Ping Chen Jing Jing Wang Jing Jing Wang Jing Jing Wang Bin Hong Ling Tan Jun Yan Jun Yan Jun Yan Zhaohuan Zhang Haiquan Liu Haiquan Liu Haiquan Liu Haiquan Liu Yingjie Pan Yingjie Pan Yingjie Pan Yong Zhao Yong Zhao Yong Zhao |
| author_sort | Ping Chen |
| collection | DOAJ |
| description | Mixed-species biofilms are the predominant form of biofilms found in nature. Research on biofilms have typically concentrated on single species biofilms and this study expands the horizon of biofilm research, where the characterization and dynamic changes of mono and mixed-species biofilms formed by the pathogens, Vibrio parahaemolyticus and Listeria monocytogenes were investigated. Compared to mono-species biofilm, the biomass, bio-volume, and thickness of mixed-species biofilms were significantly lower, which were confirmed using crystal violet staining, confocal laser scanning microscopy and scanning electron microscopy. Further experimental analysis showed these variations might result from the reduction of bacterial numbers, the down-regulation of biofilm-regulated genes and loss of metabolic activity in mixed-species biofilm. In addition, V. parahaemolyticus was located primarily on the surface layers of the mixed-species biofilms thus accruing competitive advantage. This competitive advantage was evidenced in a higher V. parahaemolyticus population density in the mixed-species biofilms. The adhesion to surfaces of the mixed-species biofilms were also reduced due to lower concentrations of extracellular polysaccharide and protein when the structure of the mixed-species was examined using Raman spectral analysis, phenol-sulfuric acid method and Lowry method. Furthermore, the minimum biofilm inhibitory concentration to antibiotics obviously decreased when V. parahaemolyticus co-exited with L. monocytogenes. This study firstly elucidated the interactive behavior in biofilm development of two foodborne pathogens, and future studies for biofilm control and antibiotic therapy should take into account interactions in mixed-species biofilms. |
| first_indexed | 2024-12-11T00:46:16Z |
| format | Article |
| id | doaj.art-0237fa87341a47bba2a3238fb60e0d90 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T00:46:16Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-0237fa87341a47bba2a3238fb60e0d902022-12-22T01:26:46ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-11-011010.3389/fmicb.2019.02543479266Characterization of Mixed-Species Biofilm Formed by Vibrio parahaemolyticus and Listeria monocytogenesPing Chen0Jing Jing Wang1Jing Jing Wang2Jing Jing Wang3Bin Hong4Ling Tan5Jun Yan6Jun Yan7Jun Yan8Zhaohuan Zhang9Haiquan Liu10Haiquan Liu11Haiquan Liu12Haiquan Liu13Yingjie Pan14Yingjie Pan15Yingjie Pan16Yong Zhao17Yong Zhao18Yong Zhao19College of Food Science and Technology, Shanghai Ocean University, Shanghai, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, ChinaShanghai Engineering Research Center of Aquatic Product Processing and Preservation, Shanghai, ChinaLaboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs, Shanghai, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, ChinaShanghai Engineering Research Center of Aquatic Product Processing and Preservation, Shanghai, ChinaLaboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs, Shanghai, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, ChinaShanghai Engineering Research Center of Aquatic Product Processing and Preservation, Shanghai, ChinaLaboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs, Shanghai, ChinaEngineering Research Center of Food Thermal-Processing Technology, Shanghai Ocean University, Shanghai, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, ChinaShanghai Engineering Research Center of Aquatic Product Processing and Preservation, Shanghai, ChinaLaboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs, Shanghai, ChinaCollege of Food Science and Technology, Shanghai Ocean University, Shanghai, ChinaShanghai Engineering Research Center of Aquatic Product Processing and Preservation, Shanghai, ChinaLaboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs, Shanghai, ChinaMixed-species biofilms are the predominant form of biofilms found in nature. Research on biofilms have typically concentrated on single species biofilms and this study expands the horizon of biofilm research, where the characterization and dynamic changes of mono and mixed-species biofilms formed by the pathogens, Vibrio parahaemolyticus and Listeria monocytogenes were investigated. Compared to mono-species biofilm, the biomass, bio-volume, and thickness of mixed-species biofilms were significantly lower, which were confirmed using crystal violet staining, confocal laser scanning microscopy and scanning electron microscopy. Further experimental analysis showed these variations might result from the reduction of bacterial numbers, the down-regulation of biofilm-regulated genes and loss of metabolic activity in mixed-species biofilm. In addition, V. parahaemolyticus was located primarily on the surface layers of the mixed-species biofilms thus accruing competitive advantage. This competitive advantage was evidenced in a higher V. parahaemolyticus population density in the mixed-species biofilms. The adhesion to surfaces of the mixed-species biofilms were also reduced due to lower concentrations of extracellular polysaccharide and protein when the structure of the mixed-species was examined using Raman spectral analysis, phenol-sulfuric acid method and Lowry method. Furthermore, the minimum biofilm inhibitory concentration to antibiotics obviously decreased when V. parahaemolyticus co-exited with L. monocytogenes. This study firstly elucidated the interactive behavior in biofilm development of two foodborne pathogens, and future studies for biofilm control and antibiotic therapy should take into account interactions in mixed-species biofilms.https://www.frontiersin.org/article/10.3389/fmicb.2019.02543/fullVibrio parahaemolyticusListeria monocytogenesfoodborne pathogenmixed-species biofilmsminimum biofilm inhibitory concentration |
| spellingShingle | Ping Chen Jing Jing Wang Jing Jing Wang Jing Jing Wang Bin Hong Ling Tan Jun Yan Jun Yan Jun Yan Zhaohuan Zhang Haiquan Liu Haiquan Liu Haiquan Liu Haiquan Liu Yingjie Pan Yingjie Pan Yingjie Pan Yong Zhao Yong Zhao Yong Zhao Characterization of Mixed-Species Biofilm Formed by Vibrio parahaemolyticus and Listeria monocytogenes Frontiers in Microbiology Vibrio parahaemolyticus Listeria monocytogenes foodborne pathogen mixed-species biofilms minimum biofilm inhibitory concentration |
| title | Characterization of Mixed-Species Biofilm Formed by Vibrio parahaemolyticus and Listeria monocytogenes |
| title_full | Characterization of Mixed-Species Biofilm Formed by Vibrio parahaemolyticus and Listeria monocytogenes |
| title_fullStr | Characterization of Mixed-Species Biofilm Formed by Vibrio parahaemolyticus and Listeria monocytogenes |
| title_full_unstemmed | Characterization of Mixed-Species Biofilm Formed by Vibrio parahaemolyticus and Listeria monocytogenes |
| title_short | Characterization of Mixed-Species Biofilm Formed by Vibrio parahaemolyticus and Listeria monocytogenes |
| title_sort | characterization of mixed species biofilm formed by vibrio parahaemolyticus and listeria monocytogenes |
| topic | Vibrio parahaemolyticus Listeria monocytogenes foodborne pathogen mixed-species biofilms minimum biofilm inhibitory concentration |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02543/full |
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