Effects of methanol extract of Galium verum L. On cardiac redox state in hypertensive rats after ischemia
Abstract The aim of this study was to assess the effects of methanol extract of G. verum on redox status of isolated heart of spontaneously hypertensive rats after ischemia. Twenty-four Wistar albino rats were divided into three groups: untreated control rats and rats that received 125 and 250 mg/kg...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2022-08-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100682&lng=en&tlng=en |
| _version_ | 1818516540683714560 |
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| author | Ana Milevic Marko Simic Marina Tomovic Marina Rankovic Vladimir Jakovljevic Jovana Bradic |
| author_facet | Ana Milevic Marko Simic Marina Tomovic Marina Rankovic Vladimir Jakovljevic Jovana Bradic |
| author_sort | Ana Milevic |
| collection | DOAJ |
| description | Abstract The aim of this study was to assess the effects of methanol extract of G. verum on redox status of isolated heart of spontaneously hypertensive rats after ischemia. Twenty-four Wistar albino rats were divided into three groups: untreated control rats and rats that received 125 and 250 mg/kg G. verum extract for 4 weeks per os. Index of lipid peroxidation (measured as TBARS) and parameters of antioxidative defence system such as level of reduced glutathione (GSH) and activities of catalase (CAT) and superoxide dismutase (SOD) were spectrophotometrically determined in heart homogenate. The index of lipid peroxidation in heart tissue was lower in both treated groups compared to the control group. On the other hand, the activity of SOD was significantly higher after consumption of both doses, while the activity of CAT was significantly higher only after treatment with a higher dose of extract. Based on our results we might conclude that 4-week treatment with methanol extracts of G. verum has the potential to modulate myocardial redox signaling after ischemia, thus significantly alleviating cardiac oxidative stress and exerting dose-dependent antioxidant properties. Future studies are certainly necessary to fully clarify the role of this plant species in myocardial I-R injury. |
| first_indexed | 2024-12-11T00:43:41Z |
| format | Article |
| id | doaj.art-023a3c6bd7a74ab28891370327b48246 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-12-11T00:43:41Z |
| publishDate | 2022-08-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-023a3c6bd7a74ab28891370327b482462022-12-22T01:26:50ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902022-08-015810.1590/s2175-97902022e191062Effects of methanol extract of Galium verum L. On cardiac redox state in hypertensive rats after ischemiaAna MilevicMarko SimicMarina TomovicMarina RankovicVladimir JakovljevicJovana Bradichttps://orcid.org/0000-0001-5388-6085Abstract The aim of this study was to assess the effects of methanol extract of G. verum on redox status of isolated heart of spontaneously hypertensive rats after ischemia. Twenty-four Wistar albino rats were divided into three groups: untreated control rats and rats that received 125 and 250 mg/kg G. verum extract for 4 weeks per os. Index of lipid peroxidation (measured as TBARS) and parameters of antioxidative defence system such as level of reduced glutathione (GSH) and activities of catalase (CAT) and superoxide dismutase (SOD) were spectrophotometrically determined in heart homogenate. The index of lipid peroxidation in heart tissue was lower in both treated groups compared to the control group. On the other hand, the activity of SOD was significantly higher after consumption of both doses, while the activity of CAT was significantly higher only after treatment with a higher dose of extract. Based on our results we might conclude that 4-week treatment with methanol extracts of G. verum has the potential to modulate myocardial redox signaling after ischemia, thus significantly alleviating cardiac oxidative stress and exerting dose-dependent antioxidant properties. Future studies are certainly necessary to fully clarify the role of this plant species in myocardial I-R injury.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100682&lng=en&tlng=enGalium verumIschemia-reperfusion injuryIsolated rat heartOxidative stress |
| spellingShingle | Ana Milevic Marko Simic Marina Tomovic Marina Rankovic Vladimir Jakovljevic Jovana Bradic Effects of methanol extract of Galium verum L. On cardiac redox state in hypertensive rats after ischemia Brazilian Journal of Pharmaceutical Sciences Galium verum Ischemia-reperfusion injury Isolated rat heart Oxidative stress |
| title | Effects of methanol extract of Galium verum L. On cardiac redox state in hypertensive rats after ischemia |
| title_full | Effects of methanol extract of Galium verum L. On cardiac redox state in hypertensive rats after ischemia |
| title_fullStr | Effects of methanol extract of Galium verum L. On cardiac redox state in hypertensive rats after ischemia |
| title_full_unstemmed | Effects of methanol extract of Galium verum L. On cardiac redox state in hypertensive rats after ischemia |
| title_short | Effects of methanol extract of Galium verum L. On cardiac redox state in hypertensive rats after ischemia |
| title_sort | effects of methanol extract of galium verum l on cardiac redox state in hypertensive rats after ischemia |
| topic | Galium verum Ischemia-reperfusion injury Isolated rat heart Oxidative stress |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502022000100682&lng=en&tlng=en |
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