Energy-efficient production of vaccine protein against porcine edema disease from transgenic lettuce (Lactuca sativa L.)
Abstract The development of functional protein production systems using transgenic plants as hosts has been rapidly progressing in recent years. Lettuce (Lactuca sativa L.) has been studied as one such host, and it has been reported that the biomass of lettuce per area and target protein expression...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-19491-z |
| _version_ | 1798001380235935744 |
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| author | Asuka Yokoyama Seika Oiwa Takeshi Matsui Kazutoshi Sawada Yasushi Tasaka Takeshi Matsumura |
| author_facet | Asuka Yokoyama Seika Oiwa Takeshi Matsui Kazutoshi Sawada Yasushi Tasaka Takeshi Matsumura |
| author_sort | Asuka Yokoyama |
| collection | DOAJ |
| description | Abstract The development of functional protein production systems using transgenic plants as hosts has been rapidly progressing in recent years. Lettuce (Lactuca sativa L.) has been studied as one such host, and it has been reported that the biomass of lettuce per area and target protein expression level can be increased by optimizing the cultivation conditions. Therefore, we investigated methods to minimize the input light energy per target protein to reduce production costs. Herein, we examined the yield of a nontoxic B subunit of Stx2e (Stx2eB) from transgenic lettuce under various cultivation conditions. Stx2eB acts as a vaccine against swine edema disease. The effects of photon flux densities (PPFDs), photoperiod, and light source on Stx2eB production were examined and the findings suggested that 400 μmol m−2 s−1, 24 h, and white LED lamps, respectively, contributed to energy-efficient Stx2eB production. In addition, Stx2eB was produced 1.4 times more efficiently per unit area time using a high plant density (228.5 plants m−2) than a common density (30.4 plants m−2). The findings of the present study can facilitate the development of energy-efficient and low-cost production processes for vaccine protein production, considering temporal and spatial perspectives. |
| first_indexed | 2024-04-11T11:35:18Z |
| format | Article |
| id | doaj.art-023aa4d14b6042af92c54b30c49cdbc7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T11:35:18Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-023aa4d14b6042af92c54b30c49cdbc72022-12-22T04:25:58ZengNature PortfolioScientific Reports2045-23222022-09-0112111110.1038/s41598-022-19491-zEnergy-efficient production of vaccine protein against porcine edema disease from transgenic lettuce (Lactuca sativa L.)Asuka Yokoyama0Seika Oiwa1Takeshi Matsui2Kazutoshi Sawada3Yasushi Tasaka4Takeshi Matsumura5Innovation Strategy and Carbon Neutral Transformation Department, Idemitsu Kosan Co., Ltd.Advanced Technology Research Laboratories, Idemitsu Kosan Co., Ltd.Advanced Technology Research Laboratories, Idemitsu Kosan Co., Ltd.Advanced Technology Research Laboratories, Idemitsu Kosan Co., Ltd.Bioproduction Research Institute, National Institute of Advanced Industrial Science and TechnologyBioproduction Research Institute, National Institute of Advanced Industrial Science and TechnologyAbstract The development of functional protein production systems using transgenic plants as hosts has been rapidly progressing in recent years. Lettuce (Lactuca sativa L.) has been studied as one such host, and it has been reported that the biomass of lettuce per area and target protein expression level can be increased by optimizing the cultivation conditions. Therefore, we investigated methods to minimize the input light energy per target protein to reduce production costs. Herein, we examined the yield of a nontoxic B subunit of Stx2e (Stx2eB) from transgenic lettuce under various cultivation conditions. Stx2eB acts as a vaccine against swine edema disease. The effects of photon flux densities (PPFDs), photoperiod, and light source on Stx2eB production were examined and the findings suggested that 400 μmol m−2 s−1, 24 h, and white LED lamps, respectively, contributed to energy-efficient Stx2eB production. In addition, Stx2eB was produced 1.4 times more efficiently per unit area time using a high plant density (228.5 plants m−2) than a common density (30.4 plants m−2). The findings of the present study can facilitate the development of energy-efficient and low-cost production processes for vaccine protein production, considering temporal and spatial perspectives.https://doi.org/10.1038/s41598-022-19491-z |
| spellingShingle | Asuka Yokoyama Seika Oiwa Takeshi Matsui Kazutoshi Sawada Yasushi Tasaka Takeshi Matsumura Energy-efficient production of vaccine protein against porcine edema disease from transgenic lettuce (Lactuca sativa L.) Scientific Reports |
| title | Energy-efficient production of vaccine protein against porcine edema disease from transgenic lettuce (Lactuca sativa L.) |
| title_full | Energy-efficient production of vaccine protein against porcine edema disease from transgenic lettuce (Lactuca sativa L.) |
| title_fullStr | Energy-efficient production of vaccine protein against porcine edema disease from transgenic lettuce (Lactuca sativa L.) |
| title_full_unstemmed | Energy-efficient production of vaccine protein against porcine edema disease from transgenic lettuce (Lactuca sativa L.) |
| title_short | Energy-efficient production of vaccine protein against porcine edema disease from transgenic lettuce (Lactuca sativa L.) |
| title_sort | energy efficient production of vaccine protein against porcine edema disease from transgenic lettuce lactuca sativa l |
| url | https://doi.org/10.1038/s41598-022-19491-z |
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