Phytochemical Characterization of Wild Hops (Humulus lupulus ssp. lupuloides) Germplasm Resources From the Maritimes Region of Canada
A survey was conducted in the Maritimes region of eastern Canada to measure the phytochemical diversity of prenylchalcone, soft resins (alpha & beta acids), and flavonol constituents from 30 unique wild-growing populations of hops (Humulus lupulus L.). Based on cone chemometrics, the majorit...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-12-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2019.01438/full |
| _version_ | 1828187461921013760 |
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| author | Jason L. McCallum Mark H. Nabuurs Spencer T. Gallant Chris W. Kirby Aaron A. S. Mills |
| author_facet | Jason L. McCallum Mark H. Nabuurs Spencer T. Gallant Chris W. Kirby Aaron A. S. Mills |
| author_sort | Jason L. McCallum |
| collection | DOAJ |
| description | A survey was conducted in the Maritimes region of eastern Canada to measure the phytochemical diversity of prenylchalcone, soft resins (alpha & beta acids), and flavonol constituents from 30 unique wild-growing populations of hops (Humulus lupulus L.). Based on cone chemometrics, the majority of accessions (63.3%) are native Humulus lupulus ssp. lupoloides, with cones containing both xanthogalenol and 4’-O-methyl xanthohumol as chemotaxonomic indicator molecules. Interestingly, the leaves of all verified Humulus lupulus ssp. lupulus accessions accumulated high proportions (>0.20 total flavonols) of two acylated flavonol derivatives (kaempferol-3-O-(6’’-O-malonyl)-β-D-glucopyranoside; quercetin-3-O-(6’’-O-malonyl)-β-D-glucopyranoside), both previously unreported from hops leaves. The native lupuloides accessions examined possess only trace amounts of this compound in their leaves (<0.10 total flavonols), suggesting its potential utility as a novel, leaf-derived chemotaxonomic marker for subspecies identification purposes. A leaf-derived taxonomic marker is useful for identifying wild-growing accessions, as leaves are present throughout the entire growing season, whereas cones are only produced late in summer. Additionally, the collection of cones from 10-meter tall wild plants in overgrown riparian habitats is often difficult. The total levels of alpha acids, beta acids, and prenylchalcones in wild-collected Maritimes lupuloides cones are markedly higher than those previously reported for lupuloides individuals in the westernmost extent of its native range and show potentially valuable traits for future cultivar development, while some may be worthy of immediate commercial release. The accessions will be maintained as a core germplasm resource for future cultivar development. |
| first_indexed | 2024-04-12T07:35:17Z |
| format | Article |
| id | doaj.art-023d36d7f3334282aa6ca03cdb041db4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T07:35:17Z |
| publishDate | 2019-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-023d36d7f3334282aa6ca03cdb041db42022-12-22T03:41:58ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2019-12-011010.3389/fpls.2019.01438479751Phytochemical Characterization of Wild Hops (Humulus lupulus ssp. lupuloides) Germplasm Resources From the Maritimes Region of CanadaJason L. McCallumMark H. NabuursSpencer T. GallantChris W. KirbyAaron A. S. MillsA survey was conducted in the Maritimes region of eastern Canada to measure the phytochemical diversity of prenylchalcone, soft resins (alpha & beta acids), and flavonol constituents from 30 unique wild-growing populations of hops (Humulus lupulus L.). Based on cone chemometrics, the majority of accessions (63.3%) are native Humulus lupulus ssp. lupoloides, with cones containing both xanthogalenol and 4’-O-methyl xanthohumol as chemotaxonomic indicator molecules. Interestingly, the leaves of all verified Humulus lupulus ssp. lupulus accessions accumulated high proportions (>0.20 total flavonols) of two acylated flavonol derivatives (kaempferol-3-O-(6’’-O-malonyl)-β-D-glucopyranoside; quercetin-3-O-(6’’-O-malonyl)-β-D-glucopyranoside), both previously unreported from hops leaves. The native lupuloides accessions examined possess only trace amounts of this compound in their leaves (<0.10 total flavonols), suggesting its potential utility as a novel, leaf-derived chemotaxonomic marker for subspecies identification purposes. A leaf-derived taxonomic marker is useful for identifying wild-growing accessions, as leaves are present throughout the entire growing season, whereas cones are only produced late in summer. Additionally, the collection of cones from 10-meter tall wild plants in overgrown riparian habitats is often difficult. The total levels of alpha acids, beta acids, and prenylchalcones in wild-collected Maritimes lupuloides cones are markedly higher than those previously reported for lupuloides individuals in the westernmost extent of its native range and show potentially valuable traits for future cultivar development, while some may be worthy of immediate commercial release. The accessions will be maintained as a core germplasm resource for future cultivar development.https://www.frontiersin.org/article/10.3389/fpls.2019.01438/fullHumulus lupulus L.lupuloideswild hopschemotaxonomykaempferol-3-O-(6’’-O-malonyl)-β-D-glucopyranosideUPLC-MS |
| spellingShingle | Jason L. McCallum Mark H. Nabuurs Spencer T. Gallant Chris W. Kirby Aaron A. S. Mills Phytochemical Characterization of Wild Hops (Humulus lupulus ssp. lupuloides) Germplasm Resources From the Maritimes Region of Canada Frontiers in Plant Science Humulus lupulus L. lupuloides wild hops chemotaxonomy kaempferol-3-O-(6’’-O-malonyl)-β-D-glucopyranoside UPLC-MS |
| title | Phytochemical Characterization of Wild Hops (Humulus lupulus ssp. lupuloides) Germplasm Resources From the Maritimes Region of Canada |
| title_full | Phytochemical Characterization of Wild Hops (Humulus lupulus ssp. lupuloides) Germplasm Resources From the Maritimes Region of Canada |
| title_fullStr | Phytochemical Characterization of Wild Hops (Humulus lupulus ssp. lupuloides) Germplasm Resources From the Maritimes Region of Canada |
| title_full_unstemmed | Phytochemical Characterization of Wild Hops (Humulus lupulus ssp. lupuloides) Germplasm Resources From the Maritimes Region of Canada |
| title_short | Phytochemical Characterization of Wild Hops (Humulus lupulus ssp. lupuloides) Germplasm Resources From the Maritimes Region of Canada |
| title_sort | phytochemical characterization of wild hops humulus lupulus ssp lupuloides germplasm resources from the maritimes region of canada |
| topic | Humulus lupulus L. lupuloides wild hops chemotaxonomy kaempferol-3-O-(6’’-O-malonyl)-β-D-glucopyranoside UPLC-MS |
| url | https://www.frontiersin.org/article/10.3389/fpls.2019.01438/full |
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