Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients
Abstract Obesity could lead to metabolic dysfunction-associated fatty liver disease (MAFLD), which severity could be linked to muscle and gut microbiota disturbances. Our prospective study enrolled 52 obese patients whose MAFLD severity was estimated by transient elastography. Patients with severe s...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-79718-9 |
| _version_ | 1818840135608827904 |
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| author | Nicolas Lanthier Julie Rodriguez Maxime Nachit Sophie Hiel Pierre Trefois Audrey M. Neyrinck Patrice D. Cani Laure B. Bindels Jean-Paul Thissen Nathalie M. Delzenne |
| author_facet | Nicolas Lanthier Julie Rodriguez Maxime Nachit Sophie Hiel Pierre Trefois Audrey M. Neyrinck Patrice D. Cani Laure B. Bindels Jean-Paul Thissen Nathalie M. Delzenne |
| author_sort | Nicolas Lanthier |
| collection | DOAJ |
| description | Abstract Obesity could lead to metabolic dysfunction-associated fatty liver disease (MAFLD), which severity could be linked to muscle and gut microbiota disturbances. Our prospective study enrolled 52 obese patients whose MAFLD severity was estimated by transient elastography. Patients with severe steatosis (n = 36) had higher ALAT values, fasting blood glucose levels as well as higher visceral adipose tissue area and skeletal muscle index evaluated by computed tomography. Patients with fibrosis (n = 13) had higher ASAT values, increased whole muscle area and lower skeletal muscle density index. In a multivariate logistic regression analysis, myosteatosis was the strongest factor associated with fibrosis. Illumina sequencing of 16S rRNA gene amplicon was performed on fecal samples. The relative abundance of fecal Clostridium sensu stricto was significantly decreased with the presence of liver fibrosis and was negatively associated with liver stiffness measurement and myosteatosis. In addition, 19 amplicon sequence variants were regulated according to the severity of the disease. Linear discriminant analysis effect size (LEfSe) also highlighted discriminant microbes in patients with fibrosis, such as an enrichment of Enterobacteriaceae and Escherichia/Shigella compared to patients with severe steatosis without fibrosis. All those data suggest a gut-liver-muscle axis in the pathogenesis of MAFLD complications. |
| first_indexed | 2024-12-19T04:05:22Z |
| format | Article |
| id | doaj.art-023ec02cb2dd45cc98da54f66da34aec |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:05:22Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-023ec02cb2dd45cc98da54f66da34aec2022-12-21T20:36:32ZengNature PortfolioScientific Reports2045-23222021-01-0111111410.1038/s41598-020-79718-9Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patientsNicolas Lanthier0Julie Rodriguez1Maxime Nachit2Sophie Hiel3Pierre Trefois4Audrey M. Neyrinck5Patrice D. Cani6Laure B. Bindels7Jean-Paul Thissen8Nathalie M. Delzenne9Laboratory of Hepatology and Gastroenterology, Institut de Recherche Expérimentale et Clinique, UCLouvain, Université catholique de LouvainMetabolism and Nutrition Research Group, Louvain Drug Research Institute, UCLouvain, Université catholique de LouvainLaboratory of Hepatology and Gastroenterology, Institut de Recherche Expérimentale et Clinique, UCLouvain, Université catholique de LouvainMetabolism and Nutrition Research Group, Louvain Drug Research Institute, UCLouvain, Université catholique de LouvainMedical and Imaging Department, Cliniques universitaires Saint-Luc, UCLouvainMetabolism and Nutrition Research Group, Louvain Drug Research Institute, UCLouvain, Université catholique de LouvainMetabolism and Nutrition Research Group, Louvain Drug Research Institute, UCLouvain, Université catholique de LouvainMetabolism and Nutrition Research Group, Louvain Drug Research Institute, UCLouvain, Université catholique de LouvainPole of Endocrinology, Diabetes and Nutrition, Institut de Recherche Expérimentale et Clinique, UCLouvain, Université catholique de LouvainMetabolism and Nutrition Research Group, Louvain Drug Research Institute, UCLouvain, Université catholique de LouvainAbstract Obesity could lead to metabolic dysfunction-associated fatty liver disease (MAFLD), which severity could be linked to muscle and gut microbiota disturbances. Our prospective study enrolled 52 obese patients whose MAFLD severity was estimated by transient elastography. Patients with severe steatosis (n = 36) had higher ALAT values, fasting blood glucose levels as well as higher visceral adipose tissue area and skeletal muscle index evaluated by computed tomography. Patients with fibrosis (n = 13) had higher ASAT values, increased whole muscle area and lower skeletal muscle density index. In a multivariate logistic regression analysis, myosteatosis was the strongest factor associated with fibrosis. Illumina sequencing of 16S rRNA gene amplicon was performed on fecal samples. The relative abundance of fecal Clostridium sensu stricto was significantly decreased with the presence of liver fibrosis and was negatively associated with liver stiffness measurement and myosteatosis. In addition, 19 amplicon sequence variants were regulated according to the severity of the disease. Linear discriminant analysis effect size (LEfSe) also highlighted discriminant microbes in patients with fibrosis, such as an enrichment of Enterobacteriaceae and Escherichia/Shigella compared to patients with severe steatosis without fibrosis. All those data suggest a gut-liver-muscle axis in the pathogenesis of MAFLD complications.https://doi.org/10.1038/s41598-020-79718-9 |
| spellingShingle | Nicolas Lanthier Julie Rodriguez Maxime Nachit Sophie Hiel Pierre Trefois Audrey M. Neyrinck Patrice D. Cani Laure B. Bindels Jean-Paul Thissen Nathalie M. Delzenne Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients Scientific Reports |
| title | Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients |
| title_full | Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients |
| title_fullStr | Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients |
| title_full_unstemmed | Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients |
| title_short | Microbiota analysis and transient elastography reveal new extra-hepatic components of liver steatosis and fibrosis in obese patients |
| title_sort | microbiota analysis and transient elastography reveal new extra hepatic components of liver steatosis and fibrosis in obese patients |
| url | https://doi.org/10.1038/s41598-020-79718-9 |
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