Enhanced Virulence Gene Activity of Agrobacterium in Muskmelon (Cucumis melo L.) cv. ‘Birdie’
<span style="font-family: Times New Roman; font-size: small;"> </span><p style="margin: 0in 0in 0pt; text-align: justify;" class="MsoNormal"><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Ga...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2011-05-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://notulaebiologicae.ro/index.php/nsb/article/view/5877 |
| Summary: | <span style="font-family: Times New Roman; font-size: small;"> </span><p style="margin: 0in 0in 0pt; text-align: justify;" class="MsoNormal"><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro Subh";"><span style="font-family: Times New Roman;">Muskmelon (</span></span><span lang="EN-US" style="color: #221e1f; font-family: "Garamond Premr Pro","serif"; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro"; mso-bidi-font-style: italic;">Cucumis melo </span><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro Subh";"><span style="font-family: Times New Roman;">L.) cultivar ‘Birdie’, was evaluated for its response to the tumorigenic </span></span><span lang="EN-US" style="color: #221e1f; font-family: "Garamond Premr Pro","serif"; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro"; mso-bidi-font-style: italic;">Agrobacterium tumefaciens </span><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro Subh";"><span style="font-family: Times New Roman;">and the oncogenic </span></span><span lang="EN-US" style="color: #221e1f; font-family: "Garamond Premr Pro","serif"; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro"; mso-bidi-font-style: italic;">A. rhizogenes </span><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro Subh";"><span style="font-family: Times New Roman;">strains. Stem and petiole of three week-old </span></span><span lang="EN-US" style="color: #221e1f; font-family: "Garamond Premr Pro","serif"; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro"; mso-bidi-font-style: italic;">in vitro</span><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro Subh";"><span style="font-family: Times New Roman;">-grown muskmelon plants were inoculated with five strains of </span></span><span lang="EN-US" style="color: #221e1f; font-family: "Garamond Premr Pro","serif"; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro"; mso-bidi-font-style: italic;">A. tumefaciens </span><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro Subh";"><span style="font-family: Times New Roman;">and </span></span><span lang="EN-US" style="color: #221e1f; font-family: "Garamond Premr Pro","serif"; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro"; mso-bidi-font-style: italic;">A. rhizogenes </span><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro Subh";"><span style="font-family: Times New Roman;">each and observed phenotypic expressions i.e. induction of crown galls and hairy roots. This phenotypic expression was efficaciously increased when virulence gene activity of different strains of two </span></span><span lang="EN-US" style="color: #221e1f; font-family: "Garamond Premr Pro","serif"; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro"; mso-bidi-font-style: italic;">Agrobacterium </span><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro Subh";"><span style="font-family: Times New Roman;">species was enhanced. Intensive studies on enhancement of virulence gene activity of </span></span><span lang="EN-US" style="color: #221e1f; font-family: "Garamond Premr Pro","serif"; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro"; mso-bidi-font-style: italic;">Agrobacterium </span><span style="font-family: Times New Roman;"><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro Subh";">found to be correlated to the appropriate light intensity (39.3 μmol m</span><span style="color: #221e1f;"><span class="A9"><span lang="EN-US" style="font-family: "Times New Roman","serif"; font-size: 5.5pt; mso-bidi-font-family: "Garamond Premr Pro Subh";">-2 </span></span><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro Subh";">s</span><span class="A9"><span lang="EN-US" style="font-family: "Times New Roman","serif"; font-size: 5.5pt; mso-bidi-font-family: "Garamond Premr Pro Subh";">-1</span></span></span><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro Subh";">) with a specific concentration of monocyclic phenolic compound, acetosyringone (20 μM). The gene activity was also influenced by several other physical factors e.g. plant tissue type, </span></span><span lang="EN-US" style="color: #221e1f; font-family: "Garamond Premr Pro","serif"; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro"; mso-bidi-font-style: italic;">Agrobacterium </span><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro Subh";"><span style="font-family: Times New Roman;">species and their strains, and plant tissue-</span></span><span lang="EN-US" style="color: #221e1f; font-family: "Garamond Premr Pro","serif"; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro"; mso-bidi-font-style: italic;">Agrobacterium </span><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro Subh";"><span style="font-family: Times New Roman;">interaction. Among the different </span></span><span lang="EN-US" style="color: #221e1f; font-family: "Garamond Premr Pro","serif"; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro"; mso-bidi-font-style: italic;">A. tumefaciens </span><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro Subh";"><span style="font-family: Times New Roman;">strains, LBA4404 showed the best virulence gene activity in both stem and petiole through the formation of higher rate of crown galls. On the other hand, strain 15834 of </span></span><span lang="EN-US" style="color: #221e1f; font-family: "Garamond Premr Pro","serif"; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro"; mso-bidi-font-style: italic;">A. rhizogenes </span><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro Subh";"><span style="font-family: Times New Roman;">showed better gene activity in stem and 8196 in petiole through the formation of higher rate of hairy roots as well as higher average number of hairy roots. Among the two different types of explants, petiole was more susceptible to both </span></span><span lang="EN-US" style="color: #221e1f; font-family: "Garamond Premr Pro","serif"; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro"; mso-bidi-font-style: italic;">Agrobacterium </span><span style="font-family: Times New Roman;"><span lang="EN-US" style="color: #221e1f; font-size: 10pt; mso-bidi-font-family: "Garamond Premr Pro Subh";">species. Thus it was concluded that future muskmelon transformation study can efficiently be carried out with LBA4404, 15834 and 8196 strains using petiole explants by adding 20 μM of acetosyringone in the medium.</span><span lang="EN-US"></span></span></p><span style="font-family: Times New Roman; font-size: small;"> </span> |
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| ISSN: | 2067-3205 2067-3264 |