The new pLAI (<it>lux </it>regulon based auto-inducible) expression system for recombinant protein production in <it>Escherichia coli</it>
<p>Abstract</p> <p>Background</p> <p>After many years of intensive research, it is generally assumed that no universal expression system can exist for high-level production of a given recombinant protein. Among the different expression systems, the inducible systems are...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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BMC
2012-01-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | http://www.microbialcellfactories.com/content/11/1/3 |
| _version_ | 1818273318436864000 |
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| author | Nocadello Salvatore Swennen Erwin |
| author_facet | Nocadello Salvatore Swennen Erwin |
| author_sort | Nocadello Salvatore |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>After many years of intensive research, it is generally assumed that no universal expression system can exist for high-level production of a given recombinant protein. Among the different expression systems, the inducible systems are the most popular for their tight regulation. However, induction is in many cases less favorable due to the high cost and/or toxicity of inducers, incompatibilities with industrial scale-up or detrimental growth conditions. Expression systems using autoinduction (or self-induction) prove to be extremely versatile allowing growth and induction of recombinant proteins without the need to monitor cell density or add inducer. Unfortunately, almost all the actual auto inducible expression systems need endogenous or induced metabolic changes during the growth to trigger induction, both frequently linked to detrimental condition to cell growth. In this context, we use a simple modular approach for a cell density-based genetic regulation in order to assemble an autoinducible recombinant protein expression system in <it>E. coli</it>.</p> <p>Result</p> <p>The newly designed pLAI expression system places the expression of recombinant proteins in <it>Escherichia coli </it>under control of the regulatory genes of the <it>lux </it>regulon of <it>Vibrio fischeri</it>'s Quorum Sensing (QS) system.</p> <p>The pLAI system allows a tight regulation of the recombinant gene allowing a negligible basal expression and expression only at high cell density. Sequence optimization of regulative genes of QS of <it>V. fischeri </it>for expression in <it>E. coli </it>upgraded the system to high level expression. Moreover, partition of regulative genes between the plasmid and the host genome and introduction of a molecular safety lock permitted tighter control of gene expression.</p> <p>Conclusion</p> <p>Coupling gene expression to cell density using cell-to-cell communication provides a promising approach for recombinant protein production. The system allows the control of expression of the target recombinant gene independently from external inducers or drastic changes in metabolic conditions and enabling tight regulation of expression.</p> |
| first_indexed | 2024-12-12T21:56:03Z |
| format | Article |
| id | doaj.art-0246822a0f39449da15616b6243faca9 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-12-12T21:56:03Z |
| publishDate | 2012-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-0246822a0f39449da15616b6243faca92022-12-22T00:10:39ZengBMCMicrobial Cell Factories1475-28592012-01-01111310.1186/1475-2859-11-3The new pLAI (<it>lux </it>regulon based auto-inducible) expression system for recombinant protein production in <it>Escherichia coli</it>Nocadello SalvatoreSwennen Erwin<p>Abstract</p> <p>Background</p> <p>After many years of intensive research, it is generally assumed that no universal expression system can exist for high-level production of a given recombinant protein. Among the different expression systems, the inducible systems are the most popular for their tight regulation. However, induction is in many cases less favorable due to the high cost and/or toxicity of inducers, incompatibilities with industrial scale-up or detrimental growth conditions. Expression systems using autoinduction (or self-induction) prove to be extremely versatile allowing growth and induction of recombinant proteins without the need to monitor cell density or add inducer. Unfortunately, almost all the actual auto inducible expression systems need endogenous or induced metabolic changes during the growth to trigger induction, both frequently linked to detrimental condition to cell growth. In this context, we use a simple modular approach for a cell density-based genetic regulation in order to assemble an autoinducible recombinant protein expression system in <it>E. coli</it>.</p> <p>Result</p> <p>The newly designed pLAI expression system places the expression of recombinant proteins in <it>Escherichia coli </it>under control of the regulatory genes of the <it>lux </it>regulon of <it>Vibrio fischeri</it>'s Quorum Sensing (QS) system.</p> <p>The pLAI system allows a tight regulation of the recombinant gene allowing a negligible basal expression and expression only at high cell density. Sequence optimization of regulative genes of QS of <it>V. fischeri </it>for expression in <it>E. coli </it>upgraded the system to high level expression. Moreover, partition of regulative genes between the plasmid and the host genome and introduction of a molecular safety lock permitted tighter control of gene expression.</p> <p>Conclusion</p> <p>Coupling gene expression to cell density using cell-to-cell communication provides a promising approach for recombinant protein production. The system allows the control of expression of the target recombinant gene independently from external inducers or drastic changes in metabolic conditions and enabling tight regulation of expression.</p>http://www.microbialcellfactories.com/content/11/1/3autoinductionrecombinant protein productionQuorum Sensing |
| spellingShingle | Nocadello Salvatore Swennen Erwin The new pLAI (<it>lux </it>regulon based auto-inducible) expression system for recombinant protein production in <it>Escherichia coli</it> Microbial Cell Factories autoinduction recombinant protein production Quorum Sensing |
| title | The new pLAI (<it>lux </it>regulon based auto-inducible) expression system for recombinant protein production in <it>Escherichia coli</it> |
| title_full | The new pLAI (<it>lux </it>regulon based auto-inducible) expression system for recombinant protein production in <it>Escherichia coli</it> |
| title_fullStr | The new pLAI (<it>lux </it>regulon based auto-inducible) expression system for recombinant protein production in <it>Escherichia coli</it> |
| title_full_unstemmed | The new pLAI (<it>lux </it>regulon based auto-inducible) expression system for recombinant protein production in <it>Escherichia coli</it> |
| title_short | The new pLAI (<it>lux </it>regulon based auto-inducible) expression system for recombinant protein production in <it>Escherichia coli</it> |
| title_sort | new plai it lux it regulon based auto inducible expression system for recombinant protein production in it escherichia coli it |
| topic | autoinduction recombinant protein production Quorum Sensing |
| url | http://www.microbialcellfactories.com/content/11/1/3 |
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