RNA‐seq bulked segregant analysis combined with KASP genotyping rapidly identified PmCH7087 as responsible for powdery mildew resistance in wheat
Abstract Powdery mildew causes considerable yield losses in common wheat (Triticum aestivum L.) production. Mapping and cloning powdery mildew‐resistant quantitative trait loci can benefit stable yield production by facilitating the breeding of resistant varieties. In this study, we used the powdery...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-11-01
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| Series: | The Plant Genome |
| Online Access: | https://doi.org/10.1002/tpg2.20120 |
| _version_ | 1818462280031928320 |
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| author | Haixian Zhan Yingli Wang Dan Zhang Chenhui Du Xiaojun Zhang Xiaoli Liu Guangyuan Wang Shuosheng Zhang |
| author_facet | Haixian Zhan Yingli Wang Dan Zhang Chenhui Du Xiaojun Zhang Xiaoli Liu Guangyuan Wang Shuosheng Zhang |
| author_sort | Haixian Zhan |
| collection | DOAJ |
| description | Abstract Powdery mildew causes considerable yield losses in common wheat (Triticum aestivum L.) production. Mapping and cloning powdery mildew‐resistant quantitative trait loci can benefit stable yield production by facilitating the breeding of resistant varieties. In this study, we used the powdery mildew resistance introgression line ‘CH7087’ (harboring the resistance gene PmCH7087) and developed a large F2 population and a corresponding F2:3 segregation population containing 2,000 family lines for molecular mapping of PmCH7087. Genetic analysis demonstrated that the resistance phenotype was controlled by a single dominant gene. According to the performance exhibited by the F2:3 lines, 50 resistant lines and 50 susceptible lines without phenotype segregation were chosen for pooling and bulked segregant RNA sequencing (BSR‐Seq) analysis. A region spanning 42.77 Mb was identified, and genotyping of an additional 183 F2:3 lines with extreme phenotypes using 20 kompetitive allele‐specific polymerase chain reaction (KASP) markers in the BSR‐Seq mapping regions confirmed this region and narrowed it to 9.68 Mb, in which 45 genes were identified and annotated. Five of these transcripts harbored nonsynonymous single nucleotide polymorphisms between the two parents, with the transcripts of TraesCS2B01G302800 being involved in signal transduction. Furthermore, TraesCS2B01G302800.2 was annotated as the closest homologue of serine/threonine‐protein kinase PBS1, a typical participant in the plant disease immune response, indicating that TraesCS2B01G302800 was the candidate gene of PmCH7087. Our results may facilitate future research attempting to improve powdery mildew resistance in wheat and to identify candidate genes for further verification and gene cloning. |
| first_indexed | 2024-12-14T23:59:31Z |
| format | Article |
| id | doaj.art-02470802bab04f7f9675850f78349c1c |
| institution | Directory Open Access Journal |
| issn | 1940-3372 |
| language | English |
| last_indexed | 2024-12-14T23:59:31Z |
| publishDate | 2021-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Plant Genome |
| spelling | doaj.art-02470802bab04f7f9675850f78349c1c2022-12-21T22:42:58ZengWileyThe Plant Genome1940-33722021-11-01143n/an/a10.1002/tpg2.20120RNA‐seq bulked segregant analysis combined with KASP genotyping rapidly identified PmCH7087 as responsible for powdery mildew resistance in wheatHaixian Zhan0Yingli Wang1Dan Zhang2Chenhui Du3Xiaojun Zhang4Xiaoli Liu5Guangyuan Wang6Shuosheng Zhang7Institute of Pharmaceutical & Food Engineering Shanxi Univ. of Chinese Medicine Jingzhong 030619 ChinaInstitute of Pharmaceutical & Food Engineering Shanxi Univ. of Chinese Medicine Jingzhong 030619 ChinaInstitute of Pharmaceutical & Food Engineering Shanxi Univ. of Chinese Medicine Jingzhong 030619 ChinaInstitute of Pharmaceutical & Food Engineering Shanxi Univ. of Chinese Medicine Jingzhong 030619 ChinaCollege of Agriculture Shanxi Agricultural Univ. Taiyuan 030032 ChinaInstitute of Pharmaceutical & Food Engineering Shanxi Univ. of Chinese Medicine Jingzhong 030619 ChinaCollege of Agriculture Shanxi Agricultural Univ. Taiyuan 030032 ChinaInstitute of Pharmaceutical & Food Engineering Shanxi Univ. of Chinese Medicine Jingzhong 030619 ChinaAbstract Powdery mildew causes considerable yield losses in common wheat (Triticum aestivum L.) production. Mapping and cloning powdery mildew‐resistant quantitative trait loci can benefit stable yield production by facilitating the breeding of resistant varieties. In this study, we used the powdery mildew resistance introgression line ‘CH7087’ (harboring the resistance gene PmCH7087) and developed a large F2 population and a corresponding F2:3 segregation population containing 2,000 family lines for molecular mapping of PmCH7087. Genetic analysis demonstrated that the resistance phenotype was controlled by a single dominant gene. According to the performance exhibited by the F2:3 lines, 50 resistant lines and 50 susceptible lines without phenotype segregation were chosen for pooling and bulked segregant RNA sequencing (BSR‐Seq) analysis. A region spanning 42.77 Mb was identified, and genotyping of an additional 183 F2:3 lines with extreme phenotypes using 20 kompetitive allele‐specific polymerase chain reaction (KASP) markers in the BSR‐Seq mapping regions confirmed this region and narrowed it to 9.68 Mb, in which 45 genes were identified and annotated. Five of these transcripts harbored nonsynonymous single nucleotide polymorphisms between the two parents, with the transcripts of TraesCS2B01G302800 being involved in signal transduction. Furthermore, TraesCS2B01G302800.2 was annotated as the closest homologue of serine/threonine‐protein kinase PBS1, a typical participant in the plant disease immune response, indicating that TraesCS2B01G302800 was the candidate gene of PmCH7087. Our results may facilitate future research attempting to improve powdery mildew resistance in wheat and to identify candidate genes for further verification and gene cloning.https://doi.org/10.1002/tpg2.20120 |
| spellingShingle | Haixian Zhan Yingli Wang Dan Zhang Chenhui Du Xiaojun Zhang Xiaoli Liu Guangyuan Wang Shuosheng Zhang RNA‐seq bulked segregant analysis combined with KASP genotyping rapidly identified PmCH7087 as responsible for powdery mildew resistance in wheat The Plant Genome |
| title | RNA‐seq bulked segregant analysis combined with KASP genotyping rapidly identified PmCH7087 as responsible for powdery mildew resistance in wheat |
| title_full | RNA‐seq bulked segregant analysis combined with KASP genotyping rapidly identified PmCH7087 as responsible for powdery mildew resistance in wheat |
| title_fullStr | RNA‐seq bulked segregant analysis combined with KASP genotyping rapidly identified PmCH7087 as responsible for powdery mildew resistance in wheat |
| title_full_unstemmed | RNA‐seq bulked segregant analysis combined with KASP genotyping rapidly identified PmCH7087 as responsible for powdery mildew resistance in wheat |
| title_short | RNA‐seq bulked segregant analysis combined with KASP genotyping rapidly identified PmCH7087 as responsible for powdery mildew resistance in wheat |
| title_sort | rna seq bulked segregant analysis combined with kasp genotyping rapidly identified pmch7087 as responsible for powdery mildew resistance in wheat |
| url | https://doi.org/10.1002/tpg2.20120 |
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