Morphological and molecular characterization of Puccinia kuehnii, the causal agent of sugarcane orange rust, in Cub
ABSTRACT Sugarcane orange rust caused by Puccinia kuehnii has recently become an important disease in sugarcane crops and its spread is causing great concern to growers. In this study, we analyzed spores from symptomatic orange rust sugarcane leaves collected in multiple locations in Cuba in a 4-yea...
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
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| Series: | Scientia Agricola |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201200&lng=en&tlng=en |
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| author | María Francisca Perera Romina Priscila Bertani Marta Eugenia Arias María de la Luz La O Hechavarría María de los Ángeles Zardón Navarro Mario Alberto Debes Ana Catalina Luque María Inés Cuenya Ricardo Acevedo Rojas Atilio Pedro Castagnaro |
| author_facet | María Francisca Perera Romina Priscila Bertani Marta Eugenia Arias María de la Luz La O Hechavarría María de los Ángeles Zardón Navarro Mario Alberto Debes Ana Catalina Luque María Inés Cuenya Ricardo Acevedo Rojas Atilio Pedro Castagnaro |
| author_sort | María Francisca Perera |
| collection | DOAJ |
| description | ABSTRACT Sugarcane orange rust caused by Puccinia kuehnii has recently become an important disease in sugarcane crops and its spread is causing great concern to growers. In this study, we analyzed spores from symptomatic orange rust sugarcane leaves collected in multiple locations in Cuba in a 4-year-period in order to characterize morphological traits of P. kuehnii, establish an adequate molecular technique to characterize it, and determine its infection court in sugarcane. Orange rust caused by P. kuehnii was confirmed by polymerase chain reaction (PCR) and morphological characterization. AFLP markers detected high diversity in P. kuenhnii samples. Sequencing of rDNA regions, as expected, did not reveal differences and SSR markers designed for P. melanocephala could not be transferred to P. kuehnii. In addition to stomata, entry through prickles was also detected as a new infection court in sugarcane. Although the presence of pustules on the adaxial leaf surface was frequently detected, no clear correlation between this presence and density of stomata and/or prickles was found. |
| first_indexed | 2024-12-10T15:48:24Z |
| format | Article |
| id | doaj.art-02489dc72ffd44d38d1d1eeb435bfbca |
| institution | Directory Open Access Journal |
| issn | 1678-992X |
| language | English |
| last_indexed | 2024-12-10T15:48:24Z |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Scientia Agricola |
| spelling | doaj.art-02489dc72ffd44d38d1d1eeb435bfbca2022-12-22T01:42:53ZengUniversidade de São PauloScientia Agricola1678-992X77210.1590/1678-992x-2018-0038S0103-90162020000201200Morphological and molecular characterization of Puccinia kuehnii, the causal agent of sugarcane orange rust, in CubMaría Francisca PereraRomina Priscila BertaniMarta Eugenia AriasMaría de la Luz La O HechavarríaMaría de los Ángeles Zardón NavarroMario Alberto DebesAna Catalina LuqueMaría Inés CuenyaRicardo Acevedo RojasAtilio Pedro CastagnaroABSTRACT Sugarcane orange rust caused by Puccinia kuehnii has recently become an important disease in sugarcane crops and its spread is causing great concern to growers. In this study, we analyzed spores from symptomatic orange rust sugarcane leaves collected in multiple locations in Cuba in a 4-year-period in order to characterize morphological traits of P. kuehnii, establish an adequate molecular technique to characterize it, and determine its infection court in sugarcane. Orange rust caused by P. kuehnii was confirmed by polymerase chain reaction (PCR) and morphological characterization. AFLP markers detected high diversity in P. kuenhnii samples. Sequencing of rDNA regions, as expected, did not reveal differences and SSR markers designed for P. melanocephala could not be transferred to P. kuehnii. In addition to stomata, entry through prickles was also detected as a new infection court in sugarcane. Although the presence of pustules on the adaxial leaf surface was frequently detected, no clear correlation between this presence and density of stomata and/or prickles was found.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201200&lng=en&tlng=enAFLPrDNASSRinfection courts |
| spellingShingle | María Francisca Perera Romina Priscila Bertani Marta Eugenia Arias María de la Luz La O Hechavarría María de los Ángeles Zardón Navarro Mario Alberto Debes Ana Catalina Luque María Inés Cuenya Ricardo Acevedo Rojas Atilio Pedro Castagnaro Morphological and molecular characterization of Puccinia kuehnii, the causal agent of sugarcane orange rust, in Cub Scientia Agricola AFLP rDNA SSR infection courts |
| title | Morphological and molecular characterization of Puccinia kuehnii, the causal agent of sugarcane orange rust, in Cub |
| title_full | Morphological and molecular characterization of Puccinia kuehnii, the causal agent of sugarcane orange rust, in Cub |
| title_fullStr | Morphological and molecular characterization of Puccinia kuehnii, the causal agent of sugarcane orange rust, in Cub |
| title_full_unstemmed | Morphological and molecular characterization of Puccinia kuehnii, the causal agent of sugarcane orange rust, in Cub |
| title_short | Morphological and molecular characterization of Puccinia kuehnii, the causal agent of sugarcane orange rust, in Cub |
| title_sort | morphological and molecular characterization of puccinia kuehnii the causal agent of sugarcane orange rust in cub |
| topic | AFLP rDNA SSR infection courts |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201200&lng=en&tlng=en |
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