Transcriptome Analysis Reveals the Role of GA<sub>3</sub> in Regulating the Asynchronism of Floral Bud Differentiation and Development in Heterodichogamous <i>Cyclocarya paliurus</i> (Batal.) Iljinskaja
<i>Cyclocarya paliurus</i> is an important medical plant owing to the diverse bioactive compounds in its leaves. However, the heterodichogamy with female and male functions segregation within protandry (PA) or protogyny (PG) may greatly affect seed quality and its plantations for medicin...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/12/6763 |
| _version_ | 1797486337919549440 |
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| author | Yinquan Qu Xiaolin Chen Xia Mao Peng Huang Xiangxiang Fu |
| author_facet | Yinquan Qu Xiaolin Chen Xia Mao Peng Huang Xiangxiang Fu |
| author_sort | Yinquan Qu |
| collection | DOAJ |
| description | <i>Cyclocarya paliurus</i> is an important medical plant owing to the diverse bioactive compounds in its leaves. However, the heterodichogamy with female and male functions segregation within protandry (PA) or protogyny (PG) may greatly affect seed quality and its plantations for medicinal use. To speculate on the factor playing the dominant role in regulating heterodichogamy in <i>C. paliurus</i>, based on phenotypic observations, our study performed a multi comparison transcriptome analysis on female and male buds (PG and PA types) using RNA-seq. For the female and male bud comparisons, a total of 6753 differentially expressed genes (DEGs) were detected. In addition, functional analysis revealed that these DEGs were significantly enriched in floral development, hormone, and GA-related pathways. As the dominant hormones responsible for floral differentiation and development, gibberellins (GAs) in floral buds from PG and PA types were quantified using HPLC-MS. Among the tested GAs, GA<sub>3</sub> positively regulated the physiological differentiation (S0) and germination (S2) of floral buds. The dynamic changes of GA<sub>3</sub> content and floral morphological features were consistent with the expression levels of GA-related genes. Divergences of GA<sub>3</sub> contents at S0 triggered the asynchronism of physiological differentiation between male and female buds of intramorphs (PA-M vs. PA-F and PG-F vs. PG-M). A significant difference in GA<sub>3</sub> content enlarged this asynchronism at S2. Thus, we speculate that GA<sub>3</sub> plays the dominant role in the formation of heterodichogamy in <i>C. paliurus</i>. Meanwhile, the expression patterns of GA-related DEGs, including <i>CPS</i>, <i>KO</i>, <i>GA20ox</i><i>, GA2<sub>OX</sub></i>, <i>GID1</i>, and <i>DELLA</i> genes, which play central roles in regulating flower development, coincided with heterodichogamous characteristics. These results support our speculations well, which should be further confirmed. |
| first_indexed | 2024-03-09T23:32:44Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:32:44Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-024ad9903cc94d8c8db9465310f796002023-11-23T17:06:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012312676310.3390/ijms23126763Transcriptome Analysis Reveals the Role of GA<sub>3</sub> in Regulating the Asynchronism of Floral Bud Differentiation and Development in Heterodichogamous <i>Cyclocarya paliurus</i> (Batal.) IljinskajaYinquan Qu0Xiaolin Chen1Xia Mao2Peng Huang3Xiangxiang Fu4Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, ChinaCo-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China<i>Cyclocarya paliurus</i> is an important medical plant owing to the diverse bioactive compounds in its leaves. However, the heterodichogamy with female and male functions segregation within protandry (PA) or protogyny (PG) may greatly affect seed quality and its plantations for medicinal use. To speculate on the factor playing the dominant role in regulating heterodichogamy in <i>C. paliurus</i>, based on phenotypic observations, our study performed a multi comparison transcriptome analysis on female and male buds (PG and PA types) using RNA-seq. For the female and male bud comparisons, a total of 6753 differentially expressed genes (DEGs) were detected. In addition, functional analysis revealed that these DEGs were significantly enriched in floral development, hormone, and GA-related pathways. As the dominant hormones responsible for floral differentiation and development, gibberellins (GAs) in floral buds from PG and PA types were quantified using HPLC-MS. Among the tested GAs, GA<sub>3</sub> positively regulated the physiological differentiation (S0) and germination (S2) of floral buds. The dynamic changes of GA<sub>3</sub> content and floral morphological features were consistent with the expression levels of GA-related genes. Divergences of GA<sub>3</sub> contents at S0 triggered the asynchronism of physiological differentiation between male and female buds of intramorphs (PA-M vs. PA-F and PG-F vs. PG-M). A significant difference in GA<sub>3</sub> content enlarged this asynchronism at S2. Thus, we speculate that GA<sub>3</sub> plays the dominant role in the formation of heterodichogamy in <i>C. paliurus</i>. Meanwhile, the expression patterns of GA-related DEGs, including <i>CPS</i>, <i>KO</i>, <i>GA20ox</i><i>, GA2<sub>OX</sub></i>, <i>GID1</i>, and <i>DELLA</i> genes, which play central roles in regulating flower development, coincided with heterodichogamous characteristics. These results support our speculations well, which should be further confirmed.https://www.mdpi.com/1422-0067/23/12/6763<i>Cyclocarya paliurus</i>heterodichogamyfloral bud differentiationGA-related DEGs |
| spellingShingle | Yinquan Qu Xiaolin Chen Xia Mao Peng Huang Xiangxiang Fu Transcriptome Analysis Reveals the Role of GA<sub>3</sub> in Regulating the Asynchronism of Floral Bud Differentiation and Development in Heterodichogamous <i>Cyclocarya paliurus</i> (Batal.) Iljinskaja International Journal of Molecular Sciences <i>Cyclocarya paliurus</i> heterodichogamy floral bud differentiation GA-related DEGs |
| title | Transcriptome Analysis Reveals the Role of GA<sub>3</sub> in Regulating the Asynchronism of Floral Bud Differentiation and Development in Heterodichogamous <i>Cyclocarya paliurus</i> (Batal.) Iljinskaja |
| title_full | Transcriptome Analysis Reveals the Role of GA<sub>3</sub> in Regulating the Asynchronism of Floral Bud Differentiation and Development in Heterodichogamous <i>Cyclocarya paliurus</i> (Batal.) Iljinskaja |
| title_fullStr | Transcriptome Analysis Reveals the Role of GA<sub>3</sub> in Regulating the Asynchronism of Floral Bud Differentiation and Development in Heterodichogamous <i>Cyclocarya paliurus</i> (Batal.) Iljinskaja |
| title_full_unstemmed | Transcriptome Analysis Reveals the Role of GA<sub>3</sub> in Regulating the Asynchronism of Floral Bud Differentiation and Development in Heterodichogamous <i>Cyclocarya paliurus</i> (Batal.) Iljinskaja |
| title_short | Transcriptome Analysis Reveals the Role of GA<sub>3</sub> in Regulating the Asynchronism of Floral Bud Differentiation and Development in Heterodichogamous <i>Cyclocarya paliurus</i> (Batal.) Iljinskaja |
| title_sort | transcriptome analysis reveals the role of ga sub 3 sub in regulating the asynchronism of floral bud differentiation and development in heterodichogamous i cyclocarya paliurus i batal iljinskaja |
| topic | <i>Cyclocarya paliurus</i> heterodichogamy floral bud differentiation GA-related DEGs |
| url | https://www.mdpi.com/1422-0067/23/12/6763 |
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