OsNPF5.16, a nitrate transporter gene with natural variation, is essential for rice growth and yield
Rice has a large number of nitrate or peptide transporter family (NPF) genes, but the effects of most members on rice growth and development are unknown. We report that OsNPF5.16, a nitrate transporter gene with natural variation in its promoter sequence, is essential for rice growth and yield. The...
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KeAi Communications Co., Ltd.
2022-04-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514121001707 |
| _version_ | 1819159464935161856 |
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| author | Jie Wang Renjing Wan Haipeng Nie Shaowu Xue Zhongming Fang |
| author_facet | Jie Wang Renjing Wan Haipeng Nie Shaowu Xue Zhongming Fang |
| author_sort | Jie Wang |
| collection | DOAJ |
| description | Rice has a large number of nitrate or peptide transporter family (NPF) genes, but the effects of most members on rice growth and development are unknown. We report that OsNPF5.16, a nitrate transporter gene with natural variation in its promoter sequence, is essential for rice growth and yield. The promoter sequence showed various differences between indica and japonica cultivars, and higher expression of OsNPF5.16 was found in indica cultivars with higher plant weight and more tillers than japonica cultivars. OsNPF5.16 was highly expressed in roots, tiller basal parts, and leaf sheaths, and its protein was localized on the plasma membrane. In cRNA-injected Xenopus laevis oocytes, OsNPF5.16 transport of nitrate at high nitrate concentration depended on pH. Overexpression of OsNPF5.16 increased nitrate content and total nitrogen content in leaf sheath as well as biomass and tiller bud length in rice. Elevated expression of OsNPF5.16 increased rice tiller number and grain yield by regulating cytokinin levels. Inhibition of OsNPF5.16 expression showed the opposite effects. Regulating OsNPF5.16 expression has potential for improving rice grain yield. |
| first_indexed | 2024-12-22T16:40:59Z |
| format | Article |
| id | doaj.art-024b8d027d984c45af114ec8d5b0bdaa |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
| language | English |
| last_indexed | 2024-12-22T16:40:59Z |
| publishDate | 2022-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj.art-024b8d027d984c45af114ec8d5b0bdaa2022-12-21T18:19:52ZengKeAi Communications Co., Ltd.Crop Journal2214-51412022-04-01102397406OsNPF5.16, a nitrate transporter gene with natural variation, is essential for rice growth and yieldJie Wang0Renjing Wan1Haipeng Nie2Shaowu Xue3Zhongming Fang4Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Agricultural Sciences, Guizhou University, Guiyang 550025, Guizhou, China; Center of Applied Biotechnology, Wuhan University of Bioengineering, Wuhan 430415, Hubei, ChinaCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, ChinaCenter of Applied Biotechnology, Wuhan University of Bioengineering, Wuhan 430415, Hubei, ChinaCollege of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, ChinaKey Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Agricultural Sciences, Guizhou University, Guiyang 550025, Guizhou, China; Center of Applied Biotechnology, Wuhan University of Bioengineering, Wuhan 430415, Hubei, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China; Corresponding author.Rice has a large number of nitrate or peptide transporter family (NPF) genes, but the effects of most members on rice growth and development are unknown. We report that OsNPF5.16, a nitrate transporter gene with natural variation in its promoter sequence, is essential for rice growth and yield. The promoter sequence showed various differences between indica and japonica cultivars, and higher expression of OsNPF5.16 was found in indica cultivars with higher plant weight and more tillers than japonica cultivars. OsNPF5.16 was highly expressed in roots, tiller basal parts, and leaf sheaths, and its protein was localized on the plasma membrane. In cRNA-injected Xenopus laevis oocytes, OsNPF5.16 transport of nitrate at high nitrate concentration depended on pH. Overexpression of OsNPF5.16 increased nitrate content and total nitrogen content in leaf sheath as well as biomass and tiller bud length in rice. Elevated expression of OsNPF5.16 increased rice tiller number and grain yield by regulating cytokinin levels. Inhibition of OsNPF5.16 expression showed the opposite effects. Regulating OsNPF5.16 expression has potential for improving rice grain yield.http://www.sciencedirect.com/science/article/pii/S2214514121001707RiceOsNPF5.16Nitrate transporterNatural variationGrowthGrain yield |
| spellingShingle | Jie Wang Renjing Wan Haipeng Nie Shaowu Xue Zhongming Fang OsNPF5.16, a nitrate transporter gene with natural variation, is essential for rice growth and yield Crop Journal Rice OsNPF5.16 Nitrate transporter Natural variation Growth Grain yield |
| title | OsNPF5.16, a nitrate transporter gene with natural variation, is essential for rice growth and yield |
| title_full | OsNPF5.16, a nitrate transporter gene with natural variation, is essential for rice growth and yield |
| title_fullStr | OsNPF5.16, a nitrate transporter gene with natural variation, is essential for rice growth and yield |
| title_full_unstemmed | OsNPF5.16, a nitrate transporter gene with natural variation, is essential for rice growth and yield |
| title_short | OsNPF5.16, a nitrate transporter gene with natural variation, is essential for rice growth and yield |
| title_sort | osnpf5 16 a nitrate transporter gene with natural variation is essential for rice growth and yield |
| topic | Rice OsNPF5.16 Nitrate transporter Natural variation Growth Grain yield |
| url | http://www.sciencedirect.com/science/article/pii/S2214514121001707 |
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