Dual-mode SERS-based lateral flow assay strips for simultaneous diagnosis of SARS-CoV-2 and influenza a virus
Abstract Since COVID-19 and flu have similar symptoms, they are difficult to distinguish without an accurate diagnosis. Therefore, it is critical to quickly and accurately determine which virus was infected and take appropriate treatments when a person has an infection. This study developed a dual-m...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2022-09-01
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| Series: | Nano Convergence |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40580-022-00330-w |
| _version_ | 1811211449775685632 |
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| author | Mengdan Lu Younju Joung Chang Su Jeon Sunjoo Kim Dongeun Yong Hyowon Jang Sung Hyun Pyun Taejoon Kang Jaebum Choo |
| author_facet | Mengdan Lu Younju Joung Chang Su Jeon Sunjoo Kim Dongeun Yong Hyowon Jang Sung Hyun Pyun Taejoon Kang Jaebum Choo |
| author_sort | Mengdan Lu |
| collection | DOAJ |
| description | Abstract Since COVID-19 and flu have similar symptoms, they are difficult to distinguish without an accurate diagnosis. Therefore, it is critical to quickly and accurately determine which virus was infected and take appropriate treatments when a person has an infection. This study developed a dual-mode surface-enhanced Raman scattering (SERS)-based LFA strip that can diagnose SARS-CoV-2 and influenza A virus with high accuracy to reduce the false-negative problem of the commercial colorimetric LFA strip. Furthermore, using a single strip, it is feasible to detect SARS-CoV-2 and influenza A virus simultaneously. A clinical test was performed on 39 patient samples (28 SARS-CoV-2 positives, 6 influenza A virus positives, and 5 negatives), evaluating the clinical efficacy of the proposed dual-mode SERS-LFA strip. Our assay results for clinical samples show that the dual-mode LFA strip significantly reduced the false-negative rate for both SARS-CoV-2 and influenza A virus. |
| first_indexed | 2024-04-12T05:13:21Z |
| format | Article |
| id | doaj.art-024cfe62534c4006b0ca7b8ce873faea |
| institution | Directory Open Access Journal |
| issn | 2196-5404 |
| language | English |
| last_indexed | 2024-04-12T05:13:21Z |
| publishDate | 2022-09-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nano Convergence |
| spelling | doaj.art-024cfe62534c4006b0ca7b8ce873faea2022-12-22T03:46:41ZengSpringerOpenNano Convergence2196-54042022-09-019111210.1186/s40580-022-00330-wDual-mode SERS-based lateral flow assay strips for simultaneous diagnosis of SARS-CoV-2 and influenza a virusMengdan Lu0Younju Joung1Chang Su Jeon2Sunjoo Kim3Dongeun Yong4Hyowon Jang5Sung Hyun Pyun6Taejoon Kang7Jaebum Choo8Department of Chemistry, Chung-Ang UniversityDepartment of Chemistry, Chung-Ang UniversityR&D Center, Speclipse Inc.Department of Laboratory Medicine, Gyeongsang National University College of MedicineDepartment of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of MedicineBionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)R&D Center, Speclipse Inc.Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)Department of Chemistry, Chung-Ang UniversityAbstract Since COVID-19 and flu have similar symptoms, they are difficult to distinguish without an accurate diagnosis. Therefore, it is critical to quickly and accurately determine which virus was infected and take appropriate treatments when a person has an infection. This study developed a dual-mode surface-enhanced Raman scattering (SERS)-based LFA strip that can diagnose SARS-CoV-2 and influenza A virus with high accuracy to reduce the false-negative problem of the commercial colorimetric LFA strip. Furthermore, using a single strip, it is feasible to detect SARS-CoV-2 and influenza A virus simultaneously. A clinical test was performed on 39 patient samples (28 SARS-CoV-2 positives, 6 influenza A virus positives, and 5 negatives), evaluating the clinical efficacy of the proposed dual-mode SERS-LFA strip. Our assay results for clinical samples show that the dual-mode LFA strip significantly reduced the false-negative rate for both SARS-CoV-2 and influenza A virus.https://doi.org/10.1186/s40580-022-00330-wSurface-enhanced Raman scatteringLateral flow assay stripDual-mode assaysSARS-CoV-2Influenza a virus |
| spellingShingle | Mengdan Lu Younju Joung Chang Su Jeon Sunjoo Kim Dongeun Yong Hyowon Jang Sung Hyun Pyun Taejoon Kang Jaebum Choo Dual-mode SERS-based lateral flow assay strips for simultaneous diagnosis of SARS-CoV-2 and influenza a virus Nano Convergence Surface-enhanced Raman scattering Lateral flow assay strip Dual-mode assays SARS-CoV-2 Influenza a virus |
| title | Dual-mode SERS-based lateral flow assay strips for simultaneous diagnosis of SARS-CoV-2 and influenza a virus |
| title_full | Dual-mode SERS-based lateral flow assay strips for simultaneous diagnosis of SARS-CoV-2 and influenza a virus |
| title_fullStr | Dual-mode SERS-based lateral flow assay strips for simultaneous diagnosis of SARS-CoV-2 and influenza a virus |
| title_full_unstemmed | Dual-mode SERS-based lateral flow assay strips for simultaneous diagnosis of SARS-CoV-2 and influenza a virus |
| title_short | Dual-mode SERS-based lateral flow assay strips for simultaneous diagnosis of SARS-CoV-2 and influenza a virus |
| title_sort | dual mode sers based lateral flow assay strips for simultaneous diagnosis of sars cov 2 and influenza a virus |
| topic | Surface-enhanced Raman scattering Lateral flow assay strip Dual-mode assays SARS-CoV-2 Influenza a virus |
| url | https://doi.org/10.1186/s40580-022-00330-w |
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