Genome-wide high-resolution mapping of UV-induced mitotic recombination events in Saccharomyces cerevisiae.
In the yeast Saccharomyces cerevisiae and most other eukaryotes, mitotic recombination is important for the repair of double-stranded DNA breaks (DSBs). Mitotic recombination between homologous chromosomes can result in loss of heterozygosity (LOH). In this study, LOH events induced by ultraviolet (...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-10-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC3814309?pdf=render |
| _version_ | 1818217615536947200 |
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| author | Yi Yin Thomas D Petes |
| author_facet | Yi Yin Thomas D Petes |
| author_sort | Yi Yin |
| collection | DOAJ |
| description | In the yeast Saccharomyces cerevisiae and most other eukaryotes, mitotic recombination is important for the repair of double-stranded DNA breaks (DSBs). Mitotic recombination between homologous chromosomes can result in loss of heterozygosity (LOH). In this study, LOH events induced by ultraviolet (UV) light are mapped throughout the genome to a resolution of about 1 kb using single-nucleotide polymorphism (SNP) microarrays. UV doses that have little effect on the viability of diploid cells stimulate crossovers more than 1000-fold in wild-type cells. In addition, UV stimulates recombination in G1-synchronized cells about 10-fold more efficiently than in G2-synchronized cells. Importantly, at high doses of UV, most conversion events reflect the repair of two sister chromatids that are broken at approximately the same position whereas at low doses, most conversion events reflect the repair of a single broken chromatid. Genome-wide mapping of about 380 unselected crossovers, break-induced replication (BIR) events, and gene conversions shows that UV-induced recombination events occur throughout the genome without pronounced hotspots, although the ribosomal RNA gene cluster has a significantly lower frequency of crossovers. |
| first_indexed | 2024-12-12T07:10:41Z |
| format | Article |
| id | doaj.art-024d28ccf6c047139c193c36175fa180 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-12T07:10:41Z |
| publishDate | 2013-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-024d28ccf6c047139c193c36175fa1802022-12-22T00:33:38ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042013-10-01910e100389410.1371/journal.pgen.1003894Genome-wide high-resolution mapping of UV-induced mitotic recombination events in Saccharomyces cerevisiae.Yi YinThomas D PetesIn the yeast Saccharomyces cerevisiae and most other eukaryotes, mitotic recombination is important for the repair of double-stranded DNA breaks (DSBs). Mitotic recombination between homologous chromosomes can result in loss of heterozygosity (LOH). In this study, LOH events induced by ultraviolet (UV) light are mapped throughout the genome to a resolution of about 1 kb using single-nucleotide polymorphism (SNP) microarrays. UV doses that have little effect on the viability of diploid cells stimulate crossovers more than 1000-fold in wild-type cells. In addition, UV stimulates recombination in G1-synchronized cells about 10-fold more efficiently than in G2-synchronized cells. Importantly, at high doses of UV, most conversion events reflect the repair of two sister chromatids that are broken at approximately the same position whereas at low doses, most conversion events reflect the repair of a single broken chromatid. Genome-wide mapping of about 380 unselected crossovers, break-induced replication (BIR) events, and gene conversions shows that UV-induced recombination events occur throughout the genome without pronounced hotspots, although the ribosomal RNA gene cluster has a significantly lower frequency of crossovers.http://europepmc.org/articles/PMC3814309?pdf=render |
| spellingShingle | Yi Yin Thomas D Petes Genome-wide high-resolution mapping of UV-induced mitotic recombination events in Saccharomyces cerevisiae. PLoS Genetics |
| title | Genome-wide high-resolution mapping of UV-induced mitotic recombination events in Saccharomyces cerevisiae. |
| title_full | Genome-wide high-resolution mapping of UV-induced mitotic recombination events in Saccharomyces cerevisiae. |
| title_fullStr | Genome-wide high-resolution mapping of UV-induced mitotic recombination events in Saccharomyces cerevisiae. |
| title_full_unstemmed | Genome-wide high-resolution mapping of UV-induced mitotic recombination events in Saccharomyces cerevisiae. |
| title_short | Genome-wide high-resolution mapping of UV-induced mitotic recombination events in Saccharomyces cerevisiae. |
| title_sort | genome wide high resolution mapping of uv induced mitotic recombination events in saccharomyces cerevisiae |
| url | http://europepmc.org/articles/PMC3814309?pdf=render |
| work_keys_str_mv | AT yiyin genomewidehighresolutionmappingofuvinducedmitoticrecombinationeventsinsaccharomycescerevisiae AT thomasdpetes genomewidehighresolutionmappingofuvinducedmitoticrecombinationeventsinsaccharomycescerevisiae |