Influence of Oral Microbiota on the Presence of IgA Anti-Citrullinated Protein Antibodies in Gingival Crevicular Fluid
IntroductionThe relation between rheumatoid arthritis (RA) and periodontitis (PD) has been investigated ever since the discovery of the citrullinating enzyme peptidyl arginine deaminase presents in the oral bacterium Porphyromonas gingivalis. Recently, we demonstrated the presence of RA autoantibodi...
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Oral Health |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/froh.2022.904711/full |
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| author | Menke J. de Smit Menke J. de Smit Poerwati Soetji Rahajoe Elisabeth Raveling-Eelsing Paola Lisotto Hermie J. M. Harmsen Nyoman Kertia Arjan Vissink Johanna Westra |
| author_facet | Menke J. de Smit Menke J. de Smit Poerwati Soetji Rahajoe Elisabeth Raveling-Eelsing Paola Lisotto Hermie J. M. Harmsen Nyoman Kertia Arjan Vissink Johanna Westra |
| author_sort | Menke J. de Smit |
| collection | DOAJ |
| description | IntroductionThe relation between rheumatoid arthritis (RA) and periodontitis (PD) has been investigated ever since the discovery of the citrullinating enzyme peptidyl arginine deaminase presents in the oral bacterium Porphyromonas gingivalis. Recently, we demonstrated the presence of RA autoantibodies, especially of IgA anti-citrullinated protein antibody (ACPA), in gingival crevicular fluid (GCF) of Indonesian patients with and without RA or PD which might indicate the local formation of RA antibodies in the periodontium.AimThe purpose of this study was to assess whether the subgingival microbiome is related to the presence of IgA ACPA in the GCF of healthy individuals with or without PD.Patients and MethodsHealthy individuals with a known periodontal status and high IgA ACPA (>0.1 U/ml) in GCF (n = 27) were selected and matched for age, gender, periodontal status, and smoking status with 27 healthy individuals without IgA ACPA in their GCF. Taxonomic profiling of the subgingival microbiome was based on bacterial 16S rRNA gene sequencing. Downstream analyses were performed to assess compositional differences between healthy subjects with or without IgA ACPA in GCF and with or without PD.ResultsBetween groups with or without PD, or with or without IgA ACPA in GCF, no differences in alpha diversity were seen. Beta diversity was different between groups with or without PD (p < 0.0001), and a trend was seen in subjects with PD between subjects with or without IgA ACPA in GCF (p = 0.084). Linear discriminant analysis effect size (LEfSe) revealed no significant differences in the total population between subjects with IgA ACPA compared to subjects without IgA ACPA in GCF. Although Porphyromonas was not identified by LEfSe, its relative abundance was significantly higher in healthy individuals with high IgA ACPA in GCF compared to individuals without IgA ACPA in GCF (p = 0.0363). Zooming in on the subgroup with PD, LEfSe revealed that species Neisseriaceae, Tannerella, and Haemophilus were more abundant in the subjects with IgA ACPA in GCF compared to subjects without IgA ACPA in GCF.ConclusionPeriodontitis and certain taxa, including Porphyromonas, seem to be associated with the local presence of ACPA in the periodontium. |
| first_indexed | 2024-04-13T20:48:38Z |
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| issn | 2673-4842 |
| language | English |
| last_indexed | 2024-04-13T20:48:38Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Oral Health |
| spelling | doaj.art-024ff6171378496ebf71e8ee070e8a2e2022-12-22T02:30:36ZengFrontiers Media S.A.Frontiers in Oral Health2673-48422022-06-01310.3389/froh.2022.904711904711Influence of Oral Microbiota on the Presence of IgA Anti-Citrullinated Protein Antibodies in Gingival Crevicular FluidMenke J. de Smit0Menke J. de Smit1Poerwati Soetji Rahajoe2Elisabeth Raveling-Eelsing3Paola Lisotto4Hermie J. M. Harmsen5Nyoman Kertia6Arjan Vissink7Johanna Westra8Department of Dentistry, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsDepartment of Oral and Maxillofacial Surgery, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsDepartment of Oral Surgery, Dr. Sardjito General Hospital, Yogyakarta, IndonesiaDepartment of Rheumatology and Clinical Immunology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsDepartment of Medical Microbiology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsDepartment of Medical Microbiology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsDepartment of Rheumatology, Dr. Sardjito General Hospital, Yogyakarta, IndonesiaDepartment of Oral and Maxillofacial Surgery, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsDepartment of Rheumatology and Clinical Immunology, University Medical Center Groningen, University of Groningen, Groningen, NetherlandsIntroductionThe relation between rheumatoid arthritis (RA) and periodontitis (PD) has been investigated ever since the discovery of the citrullinating enzyme peptidyl arginine deaminase presents in the oral bacterium Porphyromonas gingivalis. Recently, we demonstrated the presence of RA autoantibodies, especially of IgA anti-citrullinated protein antibody (ACPA), in gingival crevicular fluid (GCF) of Indonesian patients with and without RA or PD which might indicate the local formation of RA antibodies in the periodontium.AimThe purpose of this study was to assess whether the subgingival microbiome is related to the presence of IgA ACPA in the GCF of healthy individuals with or without PD.Patients and MethodsHealthy individuals with a known periodontal status and high IgA ACPA (>0.1 U/ml) in GCF (n = 27) were selected and matched for age, gender, periodontal status, and smoking status with 27 healthy individuals without IgA ACPA in their GCF. Taxonomic profiling of the subgingival microbiome was based on bacterial 16S rRNA gene sequencing. Downstream analyses were performed to assess compositional differences between healthy subjects with or without IgA ACPA in GCF and with or without PD.ResultsBetween groups with or without PD, or with or without IgA ACPA in GCF, no differences in alpha diversity were seen. Beta diversity was different between groups with or without PD (p < 0.0001), and a trend was seen in subjects with PD between subjects with or without IgA ACPA in GCF (p = 0.084). Linear discriminant analysis effect size (LEfSe) revealed no significant differences in the total population between subjects with IgA ACPA compared to subjects without IgA ACPA in GCF. Although Porphyromonas was not identified by LEfSe, its relative abundance was significantly higher in healthy individuals with high IgA ACPA in GCF compared to individuals without IgA ACPA in GCF (p = 0.0363). Zooming in on the subgroup with PD, LEfSe revealed that species Neisseriaceae, Tannerella, and Haemophilus were more abundant in the subjects with IgA ACPA in GCF compared to subjects without IgA ACPA in GCF.ConclusionPeriodontitis and certain taxa, including Porphyromonas, seem to be associated with the local presence of ACPA in the periodontium.https://www.frontiersin.org/articles/10.3389/froh.2022.904711/fullrheumatoid arthritisanti-citrullinated protein antibodies (ACPA)periodontitismucosal inflammationmicrobiome and dysbiosis |
| spellingShingle | Menke J. de Smit Menke J. de Smit Poerwati Soetji Rahajoe Elisabeth Raveling-Eelsing Paola Lisotto Hermie J. M. Harmsen Nyoman Kertia Arjan Vissink Johanna Westra Influence of Oral Microbiota on the Presence of IgA Anti-Citrullinated Protein Antibodies in Gingival Crevicular Fluid Frontiers in Oral Health rheumatoid arthritis anti-citrullinated protein antibodies (ACPA) periodontitis mucosal inflammation microbiome and dysbiosis |
| title | Influence of Oral Microbiota on the Presence of IgA Anti-Citrullinated Protein Antibodies in Gingival Crevicular Fluid |
| title_full | Influence of Oral Microbiota on the Presence of IgA Anti-Citrullinated Protein Antibodies in Gingival Crevicular Fluid |
| title_fullStr | Influence of Oral Microbiota on the Presence of IgA Anti-Citrullinated Protein Antibodies in Gingival Crevicular Fluid |
| title_full_unstemmed | Influence of Oral Microbiota on the Presence of IgA Anti-Citrullinated Protein Antibodies in Gingival Crevicular Fluid |
| title_short | Influence of Oral Microbiota on the Presence of IgA Anti-Citrullinated Protein Antibodies in Gingival Crevicular Fluid |
| title_sort | influence of oral microbiota on the presence of iga anti citrullinated protein antibodies in gingival crevicular fluid |
| topic | rheumatoid arthritis anti-citrullinated protein antibodies (ACPA) periodontitis mucosal inflammation microbiome and dysbiosis |
| url | https://www.frontiersin.org/articles/10.3389/froh.2022.904711/full |
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