Balanophora polysaccharide decreases the expression of BDNF and TrkB in liver tissues of rat with experimental liver injury
Objective To study the effects of Balanophora polysaccharides (BPS) on the expression of BDNF, TrkB and other apoptosis-related proteins in rats with experimental liver injury induced by D-gal. Methods Rats were randomly divided into control group, D-gal group (200 mg/kg of hypodermic D- galactose),...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-01-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-1-62.pdf |
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| author | XIE Ya, CHEN Ying, XIA De-yao, TANG Xian-e, WANG Feng-jie, CHEN Xian-bing |
| author_facet | XIE Ya, CHEN Ying, XIA De-yao, TANG Xian-e, WANG Feng-jie, CHEN Xian-bing |
| author_sort | XIE Ya, CHEN Ying, XIA De-yao, TANG Xian-e, WANG Feng-jie, CHEN Xian-bing |
| collection | DOAJ |
| description | Objective To study the effects of Balanophora polysaccharides (BPS) on the expression of BDNF, TrkB and other apoptosis-related proteins in rats with experimental liver injury induced by D-gal. Methods Rats were randomly divided into control group, D-gal group (200 mg/kg of hypodermic D- galactose), groups with BPS of low dosage (BPS-L), medium dosage (BPS-M) and high dosage (BPS-H), which were treated with intragastric administration by 50, 100 and 200 mg/kg BPS per day, respectively. After six weeks, serum alanine transaminase(ALT) and aspartate aminotransferase(AST) were measured. HE staining was used to observe liver tissue morphology. The localization of BDNF and TrkB was determined by immuno-histochemical staining. Western blot was used to detect BDNF, TrkB, Bcl-2, caspase-3 and Bax protein expression. ELISA was used to determine the malondialdehyde(MDA) content and superoxide dismutase(SOD) activity in liver tissues. Results Compared with the control group, the hepatocytes showed edema and punctate necrosis in D-gal group, with increased serum level of ALT and AST(P＜0.05). The results also showed decreased SOD activity and increased MDA content (P＜0.05), increased Bax, caspase-3, BDNF, TrkB expression (P＜0.05) and decreased Bcl-2 expression (P＜0.05). Compared to D-gal group, the hepatocyte degeneration and necrosis were obviously alleviated in BPS groups with each dose accompanied by decreased serum ALT and AST levels (P＜0.05), increased SOD activity and decreased MDA content (P＜0.05), reduced Bax and caspase-3 expression of pro-apoptotic proteins, increased expression Bcl-2 anti-apoptotic proteins (P＜0.05) and decreased expression of BDNF and TrkB (P＜0.05). Conclusions Balanophora polysaccharide had a protective effect on experimental liver injury caused by D-gal. The mechanism may be related to the decrease of the expression of BDNF and TrkB followed by inhibition of hepatocyte apoptosis. |
| first_indexed | 2024-03-08T16:55:30Z |
| format | Article |
| id | doaj.art-025404c8ee6540a9a11dcc4b4fff0e32 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:55:30Z |
| publishDate | 2022-01-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-025404c8ee6540a9a11dcc4b4fff0e322024-01-05T02:33:55ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252022-01-01421626710.16352/j.issn.1001-6325.2022.01.009Balanophora polysaccharide decreases the expression of BDNF and TrkB in liver tissues of rat with experimental liver injuryXIE Ya, CHEN Ying, XIA De-yao, TANG Xian-e, WANG Feng-jie, CHEN Xian-bing01. Department of Pathology, the Affiliated Hospital, Hubei Minzu University, Enshi 445000;;2. Department of Medicine, 3. Institute of Science and Technology, Hubei Minzu University, Enshi 445000, ChinaObjective To study the effects of Balanophora polysaccharides (BPS) on the expression of BDNF, TrkB and other apoptosis-related proteins in rats with experimental liver injury induced by D-gal. Methods Rats were randomly divided into control group, D-gal group (200 mg/kg of hypodermic D- galactose), groups with BPS of low dosage (BPS-L), medium dosage (BPS-M) and high dosage (BPS-H), which were treated with intragastric administration by 50, 100 and 200 mg/kg BPS per day, respectively. After six weeks, serum alanine transaminase(ALT) and aspartate aminotransferase(AST) were measured. HE staining was used to observe liver tissue morphology. The localization of BDNF and TrkB was determined by immuno-histochemical staining. Western blot was used to detect BDNF, TrkB, Bcl-2, caspase-3 and Bax protein expression. ELISA was used to determine the malondialdehyde(MDA) content and superoxide dismutase(SOD) activity in liver tissues. Results Compared with the control group, the hepatocytes showed edema and punctate necrosis in D-gal group, with increased serum level of ALT and AST(P＜0.05). The results also showed decreased SOD activity and increased MDA content (P＜0.05), increased Bax, caspase-3, BDNF, TrkB expression (P＜0.05) and decreased Bcl-2 expression (P＜0.05). Compared to D-gal group, the hepatocyte degeneration and necrosis were obviously alleviated in BPS groups with each dose accompanied by decreased serum ALT and AST levels (P＜0.05), increased SOD activity and decreased MDA content (P＜0.05), reduced Bax and caspase-3 expression of pro-apoptotic proteins, increased expression Bcl-2 anti-apoptotic proteins (P＜0.05) and decreased expression of BDNF and TrkB (P＜0.05). Conclusions Balanophora polysaccharide had a protective effect on experimental liver injury caused by D-gal. The mechanism may be related to the decrease of the expression of BDNF and TrkB followed by inhibition of hepatocyte apoptosis.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-1-62.pdfbalanophora polysaccharide|liver injury|apoptosis|bdnf/trkb |
| spellingShingle | XIE Ya, CHEN Ying, XIA De-yao, TANG Xian-e, WANG Feng-jie, CHEN Xian-bing Balanophora polysaccharide decreases the expression of BDNF and TrkB in liver tissues of rat with experimental liver injury Jichu yixue yu linchuang balanophora polysaccharide|liver injury|apoptosis|bdnf/trkb |
| title | Balanophora polysaccharide decreases the expression of BDNF and TrkB in liver tissues of rat with experimental liver injury |
| title_full | Balanophora polysaccharide decreases the expression of BDNF and TrkB in liver tissues of rat with experimental liver injury |
| title_fullStr | Balanophora polysaccharide decreases the expression of BDNF and TrkB in liver tissues of rat with experimental liver injury |
| title_full_unstemmed | Balanophora polysaccharide decreases the expression of BDNF and TrkB in liver tissues of rat with experimental liver injury |
| title_short | Balanophora polysaccharide decreases the expression of BDNF and TrkB in liver tissues of rat with experimental liver injury |
| title_sort | balanophora polysaccharide decreases the expression of bdnf and trkb in liver tissues of rat with experimental liver injury |
| topic | balanophora polysaccharide|liver injury|apoptosis|bdnf/trkb |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-1-62.pdf |
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