Ventrolateral Periaqueductal Gray Neurons Are Active During Urination
The ventrolateral periaqueductal gray (VLPAG) is thought to be the main PAG column for bladder control. PAG neurons (especially VLPAG neurons) and neurons in the pontine micturition center (PMC) innervating the bladder detrusor have anatomical and functional synaptic connections. The prevailing view...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fncel.2022.865186/full |
| _version_ | 1811335225757663232 |
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| author | Yu Rao Ziyan Gao Xianping Li Xing Li Jun Li Shanshan Liang Daihan Li Jinliang Zhai Junan Yan Junan Yan Junan Yan Jiwei Yao Xiaowei Chen Xiaowei Chen |
| author_facet | Yu Rao Ziyan Gao Xianping Li Xing Li Jun Li Shanshan Liang Daihan Li Jinliang Zhai Junan Yan Junan Yan Junan Yan Jiwei Yao Xiaowei Chen Xiaowei Chen |
| author_sort | Yu Rao |
| collection | DOAJ |
| description | The ventrolateral periaqueductal gray (VLPAG) is thought to be the main PAG column for bladder control. PAG neurons (especially VLPAG neurons) and neurons in the pontine micturition center (PMC) innervating the bladder detrusor have anatomical and functional synaptic connections. The prevailing viewpoint on neural control of the bladder is that PAG neurons receive information on the decision to void made by upstream brain regions, and consequently activate the PMC through their direct projections to initiate urination reflex. However, the exact location of the PMC-projecting VLPAG neurons, their activity in response to urination, and their whole-brain inputs remain unclear. Here, we identified the distribution of VLPAG neurons that may participate in control of the bladder or project to the PMC through retrograde neural tracing. Population Ca2+ signals of PMC-projecting VLPAG neurons highly correlated with bladder contractions and urination as shown by in vivo recording in freely moving animals. Using a RV-based retrograde trans-synaptic tracing strategy, morphological results showed that urination-related PMC-projecting VLPAG neurons received dense inputs from multiple urination-related higher brain areas, such as the medial preoptic area, medial prefrontal cortex, and lateral hypothalamus. Thus, our findings reveal a novel insight into the VLPAG for control of bladder function and provide a potential therapeutic midbrain node for neurogenic bladder dysfunction. |
| first_indexed | 2024-04-13T17:20:59Z |
| format | Article |
| id | doaj.art-025553093b944b0580db3b424c20c203 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-04-13T17:20:59Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-025553093b944b0580db3b424c20c2032022-12-22T02:37:59ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022022-06-011610.3389/fncel.2022.865186865186Ventrolateral Periaqueductal Gray Neurons Are Active During UrinationYu Rao0Ziyan Gao1Xianping Li2Xing Li3Jun Li4Shanshan Liang5Daihan Li6Jinliang Zhai7Junan Yan8Junan Yan9Junan Yan10Jiwei Yao11Xiaowei Chen12Xiaowei Chen13Brain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaCenter for Neurointelligence, School of Medicine, Chongqing University, Chongqing, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaSchool of Physical Science and Technology, Guangxi University, Nanning, ChinaSchool of Physical Science and Technology, Guangxi University, Nanning, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaCenter for Neurointelligence, School of Medicine, Chongqing University, Chongqing, ChinaMedical College, Guangxi University, Nanning, ChinaCenter for Neurointelligence, School of Medicine, Chongqing University, Chongqing, ChinaDepartment of Urology, Southwest Hospital, Third Military Medical University, Chongqing, ChinaGuangyang Bay Laboratory, Chongqing Institute for Brain and Intelligence, Chongqing, ChinaCenter for Neurointelligence, School of Medicine, Chongqing University, Chongqing, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing, ChinaGuangyang Bay Laboratory, Chongqing Institute for Brain and Intelligence, Chongqing, ChinaThe ventrolateral periaqueductal gray (VLPAG) is thought to be the main PAG column for bladder control. PAG neurons (especially VLPAG neurons) and neurons in the pontine micturition center (PMC) innervating the bladder detrusor have anatomical and functional synaptic connections. The prevailing viewpoint on neural control of the bladder is that PAG neurons receive information on the decision to void made by upstream brain regions, and consequently activate the PMC through their direct projections to initiate urination reflex. However, the exact location of the PMC-projecting VLPAG neurons, their activity in response to urination, and their whole-brain inputs remain unclear. Here, we identified the distribution of VLPAG neurons that may participate in control of the bladder or project to the PMC through retrograde neural tracing. Population Ca2+ signals of PMC-projecting VLPAG neurons highly correlated with bladder contractions and urination as shown by in vivo recording in freely moving animals. Using a RV-based retrograde trans-synaptic tracing strategy, morphological results showed that urination-related PMC-projecting VLPAG neurons received dense inputs from multiple urination-related higher brain areas, such as the medial preoptic area, medial prefrontal cortex, and lateral hypothalamus. Thus, our findings reveal a novel insight into the VLPAG for control of bladder function and provide a potential therapeutic midbrain node for neurogenic bladder dysfunction.https://www.frontiersin.org/articles/10.3389/fncel.2022.865186/fullfiber photometrypontine micturition centerventrolateral periaqueductal gray (vlPAG)urinationcystometryrabies virus |
| spellingShingle | Yu Rao Ziyan Gao Xianping Li Xing Li Jun Li Shanshan Liang Daihan Li Jinliang Zhai Junan Yan Junan Yan Junan Yan Jiwei Yao Xiaowei Chen Xiaowei Chen Ventrolateral Periaqueductal Gray Neurons Are Active During Urination Frontiers in Cellular Neuroscience fiber photometry pontine micturition center ventrolateral periaqueductal gray (vlPAG) urination cystometry rabies virus |
| title | Ventrolateral Periaqueductal Gray Neurons Are Active During Urination |
| title_full | Ventrolateral Periaqueductal Gray Neurons Are Active During Urination |
| title_fullStr | Ventrolateral Periaqueductal Gray Neurons Are Active During Urination |
| title_full_unstemmed | Ventrolateral Periaqueductal Gray Neurons Are Active During Urination |
| title_short | Ventrolateral Periaqueductal Gray Neurons Are Active During Urination |
| title_sort | ventrolateral periaqueductal gray neurons are active during urination |
| topic | fiber photometry pontine micturition center ventrolateral periaqueductal gray (vlPAG) urination cystometry rabies virus |
| url | https://www.frontiersin.org/articles/10.3389/fncel.2022.865186/full |
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