Impact of Acute Deltamethrin Poisoning on Rat Adrenal Glands: Biochemical and Pathomorphological Study
Background: Deltamethrin is known all over the world as an effective preparation for the control of insects. In connection with this, its role as a chemical stressor increases. The aim of the study was to determine the features of the functioning and structure of AG after a single administration of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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International Medical Research and Development Corporation
2017-09-01
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| Series: | International Journal of Biomedicine |
| Subjects: | |
| Online Access: | http://ijbm.org/articles/IJBM_7(3)_OA12.pdf |
| _version_ | 1818206804408008704 |
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| author | Eugene A. Chigrinski Taras V. Gerunov Liudmila K. Gerunova Petr P. Zolin |
| author_facet | Eugene A. Chigrinski Taras V. Gerunov Liudmila K. Gerunova Petr P. Zolin |
| author_sort | Eugene A. Chigrinski |
| collection | DOAJ |
| description | Background: Deltamethrin is known all over the world as an effective preparation for the control of insects. In connection with this, its role as a chemical stressor increases. The aim of the study was to determine the features of the functioning and structure of AG after a single administration of synthetic pyrethroid deltamethrin in experimental animals at a dose of 17.4 mg/kg (1/5 LD50).
Material and Methods: For the experiment, 88 male Wistar rats with a body weight of 240±10 g were divided into 8 groups of 10–12 animals each. Groups 1, 3, 5, and 7 were control groups, which were administered physiological solution intragastrically. The animals in Groups 2, 4, 6, and 8 received a single dose (17.4mg/kg) of deltamethrin, which corresponds to 1/5 LD50. In the serum of rats, the content of ACTH, progesterone, DHEA-sulfate, corticosterone and aldosterone was determined by EIA. Histological preparations of adrenal glands were stained with H&E, picrofuxin according to Van Gieson, and with Bismarck brown according to Shubich. On frozen sections, lipids were detected by Sudan Black B. Results: One day after intoxication, we identified an increase in adrenal mass, edema of the parenchyma and blood capillary overflow, and a large number of lipids in corticocytes. In the blood serum, the concentration of ACTH and corticosteroids increased, but their level decreased in the adrenal cortex. After 3 days, the concentration of corticosterone in the blood serum of the experimental animals remained above the control value, but the content of other hormones decreased. At the border of the cortex and the medulla of the adrenal glands, there were mast cells in a state of degranulation; the amount of lipids decreased with time. In the subsequent terms of the study, a decrease in the weight of AG with a decrease in the concentration of hormones in the blood serum and adrenal tissue was detected.
Conclusion: The intoxication of rats with deltamethrin causes morphofunctional changes in AG that characterize the development of the stress response. The hormonal background is not restored within a month, which indicates the possibility of developing post-toxic complications. |
| first_indexed | 2024-12-12T04:18:51Z |
| format | Article |
| id | doaj.art-025624ee776d468997ecb2621b1ab6a3 |
| institution | Directory Open Access Journal |
| issn | 2158-0510 2158-0529 |
| language | English |
| last_indexed | 2024-12-12T04:18:51Z |
| publishDate | 2017-09-01 |
| publisher | International Medical Research and Development Corporation |
| record_format | Article |
| series | International Journal of Biomedicine |
| spelling | doaj.art-025624ee776d468997ecb2621b1ab6a32022-12-22T00:38:23ZengInternational Medical Research and Development CorporationInternational Journal of Biomedicine2158-05102158-05292017-09-017322122510.21103/Article7(3)_OA12Impact of Acute Deltamethrin Poisoning on Rat Adrenal Glands: Biochemical and Pathomorphological StudyEugene A. Chigrinski0Taras V. Gerunov1Liudmila K. Gerunova2Petr P. Zolin3Omsk State Medical UniversityOmsk State Agrarian University named after P.A. StolypinOmsk State Agrarian University named after P.A. StolypinOmsk State Medical UniversityBackground: Deltamethrin is known all over the world as an effective preparation for the control of insects. In connection with this, its role as a chemical stressor increases. The aim of the study was to determine the features of the functioning and structure of AG after a single administration of synthetic pyrethroid deltamethrin in experimental animals at a dose of 17.4 mg/kg (1/5 LD50). Material and Methods: For the experiment, 88 male Wistar rats with a body weight of 240±10 g were divided into 8 groups of 10–12 animals each. Groups 1, 3, 5, and 7 were control groups, which were administered physiological solution intragastrically. The animals in Groups 2, 4, 6, and 8 received a single dose (17.4mg/kg) of deltamethrin, which corresponds to 1/5 LD50. In the serum of rats, the content of ACTH, progesterone, DHEA-sulfate, corticosterone and aldosterone was determined by EIA. Histological preparations of adrenal glands were stained with H&E, picrofuxin according to Van Gieson, and with Bismarck brown according to Shubich. On frozen sections, lipids were detected by Sudan Black B. Results: One day after intoxication, we identified an increase in adrenal mass, edema of the parenchyma and blood capillary overflow, and a large number of lipids in corticocytes. In the blood serum, the concentration of ACTH and corticosteroids increased, but their level decreased in the adrenal cortex. After 3 days, the concentration of corticosterone in the blood serum of the experimental animals remained above the control value, but the content of other hormones decreased. At the border of the cortex and the medulla of the adrenal glands, there were mast cells in a state of degranulation; the amount of lipids decreased with time. In the subsequent terms of the study, a decrease in the weight of AG with a decrease in the concentration of hormones in the blood serum and adrenal tissue was detected. Conclusion: The intoxication of rats with deltamethrin causes morphofunctional changes in AG that characterize the development of the stress response. The hormonal background is not restored within a month, which indicates the possibility of developing post-toxic complications.http://ijbm.org/articles/IJBM_7(3)_OA12.pdfpesticidespyrethroidsdeltamethrinadrenal glandssteroid hormones |
| spellingShingle | Eugene A. Chigrinski Taras V. Gerunov Liudmila K. Gerunova Petr P. Zolin Impact of Acute Deltamethrin Poisoning on Rat Adrenal Glands: Biochemical and Pathomorphological Study International Journal of Biomedicine pesticides pyrethroids deltamethrin adrenal glands steroid hormones |
| title | Impact of Acute Deltamethrin Poisoning on Rat Adrenal Glands: Biochemical and Pathomorphological Study |
| title_full | Impact of Acute Deltamethrin Poisoning on Rat Adrenal Glands: Biochemical and Pathomorphological Study |
| title_fullStr | Impact of Acute Deltamethrin Poisoning on Rat Adrenal Glands: Biochemical and Pathomorphological Study |
| title_full_unstemmed | Impact of Acute Deltamethrin Poisoning on Rat Adrenal Glands: Biochemical and Pathomorphological Study |
| title_short | Impact of Acute Deltamethrin Poisoning on Rat Adrenal Glands: Biochemical and Pathomorphological Study |
| title_sort | impact of acute deltamethrin poisoning on rat adrenal glands biochemical and pathomorphological study |
| topic | pesticides pyrethroids deltamethrin adrenal glands steroid hormones |
| url | http://ijbm.org/articles/IJBM_7(3)_OA12.pdf |
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