Suppression of UVB-Induced MMP-1 Expression in Human Skin Fibroblasts Using Lysate of <i>Lactobacillus iners</i> Derived from Korean Women’s Skin in Their Twenties
The process of skin aging is intricate, involving intrinsic aging, influenced by internal factors, and extrinsic aging, mainly caused by exposure to UV radiation, resulting in photoaging. Photoaging manifests as skin issues such as wrinkles and discoloration. The skin microbiome, a diverse community...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/46/1/33 |
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| author | Jin-Sung Lee Jin-Woo Min Seong-Bong Gye Yong-Woo Kim Hee-Cheol Kang Yoon-Seo Choi Won-Sang Seo Bun-Yeoul Lee |
| author_facet | Jin-Sung Lee Jin-Woo Min Seong-Bong Gye Yong-Woo Kim Hee-Cheol Kang Yoon-Seo Choi Won-Sang Seo Bun-Yeoul Lee |
| author_sort | Jin-Sung Lee |
| collection | DOAJ |
| description | The process of skin aging is intricate, involving intrinsic aging, influenced by internal factors, and extrinsic aging, mainly caused by exposure to UV radiation, resulting in photoaging. Photoaging manifests as skin issues such as wrinkles and discoloration. The skin microbiome, a diverse community of microorganisms on the skin’s surface, plays a crucial role in skin protection and can be affected by factors like humidity and pH. Probiotics, beneficial microorganisms, have been investigated for their potential to enhance skin health by regulating the skin microbiome. This can be accomplished through oral probiotics, impacting the gut–skin axis, or topical applications introducing live bacteria to the skin. Probiotics mitigate oxidative stress, suppress inflammation, and maintain the skin’s extracellular matrix, ultimately averting skin aging. However, research on probiotics derived from human skin is limited, and there is no established product for preventing photoaging. The mechanism by which probiotics shield the skin microbiome and skin layers from UV radiation remains unclear. Recently, researchers have discovered <i>Lactobacillus</i> in the skin, with reports indicating a decrease in this microorganism with age. In a recent study, scientists isolated <i>Lactobacillus iners</i> KOLBM20 from the skin of individuals in their twenties and confirmed its effectiveness. A comparative analysis of genetic sequences revealed that strain KOLBM20 belongs to the <i>Lactobacillus</i> genus and closely relates to <i>L. iners</i> DSM13335(T) with a 99.20% similarity. Importantly, <i>Lactobacillus iners</i> KOLBM20 displayed anti-wrinkle properties by inhibiting MMP-1. This investigation demonstrated the inhibitory effect of KOLBM20 strain lysate on MMP-1 expression. Moreover, the data suggest that KOLBM20 strain lysate may prevent UVB-induced MMP-1 expression by inhibiting the activation of the ERK, JNK, and p38 signaling pathways induced by UVB. Consequently, KOLBM20 strain lysate holds promise as a potential therapeutic agent for preventing and treating skin photoaging. |
| first_indexed | 2024-03-08T11:00:44Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-08T11:00:44Z |
| publishDate | 2024-01-01 |
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| spelling | doaj.art-0256c08aeb814f189ba9ecc2fe880ebe2024-01-26T15:50:45ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-01-0146151352610.3390/cimb46010033Suppression of UVB-Induced MMP-1 Expression in Human Skin Fibroblasts Using Lysate of <i>Lactobacillus iners</i> Derived from Korean Women’s Skin in Their TwentiesJin-Sung Lee0Jin-Woo Min1Seong-Bong Gye2Yong-Woo Kim3Hee-Cheol Kang4Yoon-Seo Choi5Won-Sang Seo6Bun-Yeoul Lee7Department of Applied Biotechnology, Ajou University, Suwon 16499, Republic of KoreaGreen & Biome Customizing Laboratory, GFC Co., Ltd., Hwaseong 18471, Republic of KoreaR&D Complex, Kolmar Korea, 61, 8-gil, Heolleung-ro, Seocho-gu, Seoul 06800, Republic of KoreaR&D Complex, Kolmar Korea, 61, 8-gil, Heolleung-ro, Seocho-gu, Seoul 06800, Republic of KoreaGreen & Biome Customizing Laboratory, GFC Co., Ltd., Hwaseong 18471, Republic of KoreaGraduate School-Interdisciplinary Program in Biocosmetics, Sungkyunkwan University, Suwon 16419, Republic of KoreaGreen & Biome Customizing Laboratory, GFC Co., Ltd., Hwaseong 18471, Republic of KoreaDepartment of Molecular Science and Technology, Ajou University, Suwon 16499, Republic of KoreaThe process of skin aging is intricate, involving intrinsic aging, influenced by internal factors, and extrinsic aging, mainly caused by exposure to UV radiation, resulting in photoaging. Photoaging manifests as skin issues such as wrinkles and discoloration. The skin microbiome, a diverse community of microorganisms on the skin’s surface, plays a crucial role in skin protection and can be affected by factors like humidity and pH. Probiotics, beneficial microorganisms, have been investigated for their potential to enhance skin health by regulating the skin microbiome. This can be accomplished through oral probiotics, impacting the gut–skin axis, or topical applications introducing live bacteria to the skin. Probiotics mitigate oxidative stress, suppress inflammation, and maintain the skin’s extracellular matrix, ultimately averting skin aging. However, research on probiotics derived from human skin is limited, and there is no established product for preventing photoaging. The mechanism by which probiotics shield the skin microbiome and skin layers from UV radiation remains unclear. Recently, researchers have discovered <i>Lactobacillus</i> in the skin, with reports indicating a decrease in this microorganism with age. In a recent study, scientists isolated <i>Lactobacillus iners</i> KOLBM20 from the skin of individuals in their twenties and confirmed its effectiveness. A comparative analysis of genetic sequences revealed that strain KOLBM20 belongs to the <i>Lactobacillus</i> genus and closely relates to <i>L. iners</i> DSM13335(T) with a 99.20% similarity. Importantly, <i>Lactobacillus iners</i> KOLBM20 displayed anti-wrinkle properties by inhibiting MMP-1. This investigation demonstrated the inhibitory effect of KOLBM20 strain lysate on MMP-1 expression. Moreover, the data suggest that KOLBM20 strain lysate may prevent UVB-induced MMP-1 expression by inhibiting the activation of the ERK, JNK, and p38 signaling pathways induced by UVB. Consequently, KOLBM20 strain lysate holds promise as a potential therapeutic agent for preventing and treating skin photoaging.https://www.mdpi.com/1467-3045/46/1/33<i>Lactobacillus</i>human skin derived probioticsUVB-inducedMMP-116S rRNAERK |
| spellingShingle | Jin-Sung Lee Jin-Woo Min Seong-Bong Gye Yong-Woo Kim Hee-Cheol Kang Yoon-Seo Choi Won-Sang Seo Bun-Yeoul Lee Suppression of UVB-Induced MMP-1 Expression in Human Skin Fibroblasts Using Lysate of <i>Lactobacillus iners</i> Derived from Korean Women’s Skin in Their Twenties Current Issues in Molecular Biology <i>Lactobacillus</i> human skin derived probiotics UVB-induced MMP-1 16S rRNA ERK |
| title | Suppression of UVB-Induced MMP-1 Expression in Human Skin Fibroblasts Using Lysate of <i>Lactobacillus iners</i> Derived from Korean Women’s Skin in Their Twenties |
| title_full | Suppression of UVB-Induced MMP-1 Expression in Human Skin Fibroblasts Using Lysate of <i>Lactobacillus iners</i> Derived from Korean Women’s Skin in Their Twenties |
| title_fullStr | Suppression of UVB-Induced MMP-1 Expression in Human Skin Fibroblasts Using Lysate of <i>Lactobacillus iners</i> Derived from Korean Women’s Skin in Their Twenties |
| title_full_unstemmed | Suppression of UVB-Induced MMP-1 Expression in Human Skin Fibroblasts Using Lysate of <i>Lactobacillus iners</i> Derived from Korean Women’s Skin in Their Twenties |
| title_short | Suppression of UVB-Induced MMP-1 Expression in Human Skin Fibroblasts Using Lysate of <i>Lactobacillus iners</i> Derived from Korean Women’s Skin in Their Twenties |
| title_sort | suppression of uvb induced mmp 1 expression in human skin fibroblasts using lysate of i lactobacillus iners i derived from korean women s skin in their twenties |
| topic | <i>Lactobacillus</i> human skin derived probiotics UVB-induced MMP-1 16S rRNA ERK |
| url | https://www.mdpi.com/1467-3045/46/1/33 |
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