Aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation
Abstract The development of GABAergic interneurons is important for the functional maturation of cortical circuits. After migrating into the cortex, GABAergic interneurons start to receive glutamatergic connections from cortical excitatory neurons and thus gradually become integrated into cortical c...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-82679-2 |
| _version_ | 1818846092848005120 |
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| author | Rongkang Deng Joseph P. Y. Kao Patrick O. Kanold |
| author_facet | Rongkang Deng Joseph P. Y. Kao Patrick O. Kanold |
| author_sort | Rongkang Deng |
| collection | DOAJ |
| description | Abstract The development of GABAergic interneurons is important for the functional maturation of cortical circuits. After migrating into the cortex, GABAergic interneurons start to receive glutamatergic connections from cortical excitatory neurons and thus gradually become integrated into cortical circuits. These glutamatergic connections are mediated by glutamate receptors including AMPA and NMDA receptors and the ratio of AMPA to NMDA receptors decreases during development. Since previous studies have shown that retinal input can regulate the early development of connections along the visual pathway, we investigated if the maturation of glutamatergic inputs to GABAergic interneurons in the visual cortex requires retinal input. We mapped the spatial pattern of glutamatergic connections to layer 4 (L4) GABAergic interneurons in mouse visual cortex at around postnatal day (P) 16 by laser-scanning photostimulation and investigated the effect of binocular enucleations at P1/P2 on these patterns. Gad2-positive interneurons in enucleated animals showed an increased fraction of AMPAR-mediated input from L2/3 and a decreased fraction of input from L5/6. Parvalbumin-expressing (PV) interneurons showed similar changes in relative connectivity. NMDAR-only input was largely unchanged by enucleation. Our results show that retinal input sculpts the integration of interneurons into V1 circuits and suggest that the development of AMPAR- and NMDAR-only connections might be regulated differently. |
| first_indexed | 2024-12-19T05:40:04Z |
| format | Article |
| id | doaj.art-0258e50750d14b5f9393765fc78f8ed3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T05:40:04Z |
| publishDate | 2021-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0258e50750d14b5f9393765fc78f8ed32022-12-21T20:34:01ZengNature PortfolioScientific Reports2045-23222021-02-0111111810.1038/s41598-021-82679-2Aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleationRongkang Deng0Joseph P. Y. Kao1Patrick O. Kanold2Department of Biology, University of MarylandCenter for Biomedical Engineering and Technology, and Department of Physiology, University of Maryland School of MedicineDepartment of Biomedical Engineering, Johns Hopkins UniversityAbstract The development of GABAergic interneurons is important for the functional maturation of cortical circuits. After migrating into the cortex, GABAergic interneurons start to receive glutamatergic connections from cortical excitatory neurons and thus gradually become integrated into cortical circuits. These glutamatergic connections are mediated by glutamate receptors including AMPA and NMDA receptors and the ratio of AMPA to NMDA receptors decreases during development. Since previous studies have shown that retinal input can regulate the early development of connections along the visual pathway, we investigated if the maturation of glutamatergic inputs to GABAergic interneurons in the visual cortex requires retinal input. We mapped the spatial pattern of glutamatergic connections to layer 4 (L4) GABAergic interneurons in mouse visual cortex at around postnatal day (P) 16 by laser-scanning photostimulation and investigated the effect of binocular enucleations at P1/P2 on these patterns. Gad2-positive interneurons in enucleated animals showed an increased fraction of AMPAR-mediated input from L2/3 and a decreased fraction of input from L5/6. Parvalbumin-expressing (PV) interneurons showed similar changes in relative connectivity. NMDAR-only input was largely unchanged by enucleation. Our results show that retinal input sculpts the integration of interneurons into V1 circuits and suggest that the development of AMPAR- and NMDAR-only connections might be regulated differently.https://doi.org/10.1038/s41598-021-82679-2 |
| spellingShingle | Rongkang Deng Joseph P. Y. Kao Patrick O. Kanold Aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation Scientific Reports |
| title | Aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation |
| title_full | Aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation |
| title_fullStr | Aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation |
| title_full_unstemmed | Aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation |
| title_short | Aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation |
| title_sort | aberrant development of excitatory circuits to inhibitory neurons in the primary visual cortex after neonatal binocular enucleation |
| url | https://doi.org/10.1038/s41598-021-82679-2 |
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