A Highly Integrated and Diminutive Fluorescence Detector for Point-of-Care Testing: Dual Negative Feedback Light-Emitting Diode (LED) Drive and Photoelectric Processing Circuits Design and Implementation
As an important detection tool in biochemistry, fluorescence detection has wide applications. Quantitative detection can be achieved by detecting fluorescence signals excited by excitation light at a specific wavelength range. Therefore, the key to fluorescence detection is the stable control of the...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/9/764 |
| _version_ | 1797490580041760768 |
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| author | Yue Wang Yile Fang Haoran Liu Xiangyi Su Zhu Chen Song Li Nongyue He |
| author_facet | Yue Wang Yile Fang Haoran Liu Xiangyi Su Zhu Chen Song Li Nongyue He |
| author_sort | Yue Wang |
| collection | DOAJ |
| description | As an important detection tool in biochemistry, fluorescence detection has wide applications. Quantitative detection can be achieved by detecting fluorescence signals excited by excitation light at a specific wavelength range. Therefore, the key to fluorescence detection is the stable control of the excitation light and the accurate acquisition of weak photoelectric signals. Moreover, to improve portability and instantaneity, devices are developing in miniaturization and integration. As the core of such devices, fluorescence detectors should also have these features. Under this circumstance, we designed a highly integrated and diminutive fluorescence detector and focused on its excitation light driving and photoelectric signal processing. A current–light dual negative feedback light-emitting diode (LED) driving circuit was proposed to obtain constant current and luminance. In addition, a silicon photodiode (PD) was used to receive and convert the fluorescence signal to an electric signal. Then, amplifying, filtering, and analog-to-digital (A/D) converting were applied to make the detection of weak fluorescence signals possible. The test results showed that the designed circuit has wonderful performance, and the detector shows good linearity (R<sup>2</sup> = 0.9967) and sensitivity (LOD = 0.077 nM) in the detection of <i>fluorescein sodium</i> solution. Finally, a real-time fluorescence polymerase chain reaction (real-time PCR) of <i>Legionella pneumophila</i> was carried out on a homemade platform equipped with this detector, indicating that the detector met the requirements of real-time PCR detection. |
| first_indexed | 2024-03-10T00:34:03Z |
| format | Article |
| id | doaj.art-02599e14812b491cadb976507730f090 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T00:34:03Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-02599e14812b491cadb976507730f0902023-11-23T15:18:45ZengMDPI AGBiosensors2079-63742022-09-0112976410.3390/bios12090764A Highly Integrated and Diminutive Fluorescence Detector for Point-of-Care Testing: Dual Negative Feedback Light-Emitting Diode (LED) Drive and Photoelectric Processing Circuits Design and ImplementationYue Wang0Yile Fang1Haoran Liu2Xiangyi Su3Zhu Chen4Song Li5Nongyue He6State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaEconomical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Green Chemistry and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, ChinaEconomical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Green Chemistry and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaAs an important detection tool in biochemistry, fluorescence detection has wide applications. Quantitative detection can be achieved by detecting fluorescence signals excited by excitation light at a specific wavelength range. Therefore, the key to fluorescence detection is the stable control of the excitation light and the accurate acquisition of weak photoelectric signals. Moreover, to improve portability and instantaneity, devices are developing in miniaturization and integration. As the core of such devices, fluorescence detectors should also have these features. Under this circumstance, we designed a highly integrated and diminutive fluorescence detector and focused on its excitation light driving and photoelectric signal processing. A current–light dual negative feedback light-emitting diode (LED) driving circuit was proposed to obtain constant current and luminance. In addition, a silicon photodiode (PD) was used to receive and convert the fluorescence signal to an electric signal. Then, amplifying, filtering, and analog-to-digital (A/D) converting were applied to make the detection of weak fluorescence signals possible. The test results showed that the designed circuit has wonderful performance, and the detector shows good linearity (R<sup>2</sup> = 0.9967) and sensitivity (LOD = 0.077 nM) in the detection of <i>fluorescein sodium</i> solution. Finally, a real-time fluorescence polymerase chain reaction (real-time PCR) of <i>Legionella pneumophila</i> was carried out on a homemade platform equipped with this detector, indicating that the detector met the requirements of real-time PCR detection.https://www.mdpi.com/2079-6374/12/9/764fluorescence detectionLED drive circuitphotoelectric processing circuitpoint-of-care testingreal-time fluorescence PCR |
| spellingShingle | Yue Wang Yile Fang Haoran Liu Xiangyi Su Zhu Chen Song Li Nongyue He A Highly Integrated and Diminutive Fluorescence Detector for Point-of-Care Testing: Dual Negative Feedback Light-Emitting Diode (LED) Drive and Photoelectric Processing Circuits Design and Implementation Biosensors fluorescence detection LED drive circuit photoelectric processing circuit point-of-care testing real-time fluorescence PCR |
| title | A Highly Integrated and Diminutive Fluorescence Detector for Point-of-Care Testing: Dual Negative Feedback Light-Emitting Diode (LED) Drive and Photoelectric Processing Circuits Design and Implementation |
| title_full | A Highly Integrated and Diminutive Fluorescence Detector for Point-of-Care Testing: Dual Negative Feedback Light-Emitting Diode (LED) Drive and Photoelectric Processing Circuits Design and Implementation |
| title_fullStr | A Highly Integrated and Diminutive Fluorescence Detector for Point-of-Care Testing: Dual Negative Feedback Light-Emitting Diode (LED) Drive and Photoelectric Processing Circuits Design and Implementation |
| title_full_unstemmed | A Highly Integrated and Diminutive Fluorescence Detector for Point-of-Care Testing: Dual Negative Feedback Light-Emitting Diode (LED) Drive and Photoelectric Processing Circuits Design and Implementation |
| title_short | A Highly Integrated and Diminutive Fluorescence Detector for Point-of-Care Testing: Dual Negative Feedback Light-Emitting Diode (LED) Drive and Photoelectric Processing Circuits Design and Implementation |
| title_sort | highly integrated and diminutive fluorescence detector for point of care testing dual negative feedback light emitting diode led drive and photoelectric processing circuits design and implementation |
| topic | fluorescence detection LED drive circuit photoelectric processing circuit point-of-care testing real-time fluorescence PCR |
| url | https://www.mdpi.com/2079-6374/12/9/764 |
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