Inflammatory response, parasite load and AgNOR expression in ear skin of symptomatic and asymptomatic Leishmania (Leishmania) chagasi infected dogs
The skin has an important role in the transmission of visceral leishmaniasis (VL) as the infection pathway in dogs. To better characterize the inflammatory response of intact skin in VL, sixty infected dogs (30 symptomatic and 30 asymptomatic) and six non-infected controls were studied. Diagnosis of...
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| Format: | Article |
| Language: | English |
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SciELO
2011-01-01
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| Series: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300011 |
| _version_ | 1819261753723191296 |
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| author | Verçosa BLA Borges ACJ Mendes FJM Costa MLS Pereira NB Melo MN Mendonça IL Costa FAL Vasconcelos AC |
| author_facet | Verçosa BLA Borges ACJ Mendes FJM Costa MLS Pereira NB Melo MN Mendonça IL Costa FAL Vasconcelos AC |
| author_sort | Verçosa BLA |
| collection | DOAJ |
| description | The skin has an important role in the transmission of visceral leishmaniasis (VL) as the infection pathway in dogs. To better characterize the inflammatory response of intact skin in VL, sixty infected dogs (30 symptomatic and 30 asymptomatic) and six non-infected controls were studied. Diagnosis of visceral leishmaniasis was confirmed by RIFI and ELISA; direct visualization of the parasite in bone marrow aspirate; imprints of popliteal lymph nodes, spleen, liver and skin; culture in NNN-phase liquid Schneider's medium; and PCR (performed only in the ear skin). Amastigote forms of the parasite in intact skin were found only in symptomatic dogs. Inflammatory infiltrates were observed in all groups, varying from intense and/or moderate in symptomatic to discrete and/or negligible in asymptomatic and control animals. Parasite load was associated with the intensity of the inflammatory response and with clinical manifestations in canine visceral leishmaniasis. AgNOr as active transcription markers were expressed in inflammatory cells and within apoptotic bodies in all groups, including controls, with no statistical difference. Therefore, cell activation and transcription do occur in both symptomatic and asymptomatic canine visceral leishmaniasis and may result in more necrosis and inflammation or in apoptosis and less symptoms, depending on the parasite load. |
| first_indexed | 2024-12-23T19:46:49Z |
| format | Article |
| id | doaj.art-025a77fa476c408caa802e6c6b15e1c6 |
| institution | Directory Open Access Journal |
| issn | 1678-9199 |
| language | English |
| last_indexed | 2024-12-23T19:46:49Z |
| publishDate | 2011-01-01 |
| publisher | SciELO |
| record_format | Article |
| series | Journal of Venomous Animals and Toxins including Tropical Diseases |
| spelling | doaj.art-025a77fa476c408caa802e6c6b15e1c62022-12-21T17:33:30ZengSciELOJournal of Venomous Animals and Toxins including Tropical Diseases1678-91992011-01-0117330831710.1590/S1678-91992011000300011Inflammatory response, parasite load and AgNOR expression in ear skin of symptomatic and asymptomatic Leishmania (Leishmania) chagasi infected dogsVerçosa BLABorges ACJMendes FJMCosta MLSPereira NBMelo MNMendonça ILCosta FALVasconcelos ACThe skin has an important role in the transmission of visceral leishmaniasis (VL) as the infection pathway in dogs. To better characterize the inflammatory response of intact skin in VL, sixty infected dogs (30 symptomatic and 30 asymptomatic) and six non-infected controls were studied. Diagnosis of visceral leishmaniasis was confirmed by RIFI and ELISA; direct visualization of the parasite in bone marrow aspirate; imprints of popliteal lymph nodes, spleen, liver and skin; culture in NNN-phase liquid Schneider's medium; and PCR (performed only in the ear skin). Amastigote forms of the parasite in intact skin were found only in symptomatic dogs. Inflammatory infiltrates were observed in all groups, varying from intense and/or moderate in symptomatic to discrete and/or negligible in asymptomatic and control animals. Parasite load was associated with the intensity of the inflammatory response and with clinical manifestations in canine visceral leishmaniasis. AgNOr as active transcription markers were expressed in inflammatory cells and within apoptotic bodies in all groups, including controls, with no statistical difference. Therefore, cell activation and transcription do occur in both symptomatic and asymptomatic canine visceral leishmaniasis and may result in more necrosis and inflammation or in apoptosis and less symptoms, depending on the parasite load.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300011visceral leishmaniasisinflammationparasitesLeishmania chagasi |
| spellingShingle | Verçosa BLA Borges ACJ Mendes FJM Costa MLS Pereira NB Melo MN Mendonça IL Costa FAL Vasconcelos AC Inflammatory response, parasite load and AgNOR expression in ear skin of symptomatic and asymptomatic Leishmania (Leishmania) chagasi infected dogs Journal of Venomous Animals and Toxins including Tropical Diseases visceral leishmaniasis inflammation parasites Leishmania chagasi |
| title | Inflammatory response, parasite load and AgNOR expression in ear skin of symptomatic and asymptomatic Leishmania (Leishmania) chagasi infected dogs |
| title_full | Inflammatory response, parasite load and AgNOR expression in ear skin of symptomatic and asymptomatic Leishmania (Leishmania) chagasi infected dogs |
| title_fullStr | Inflammatory response, parasite load and AgNOR expression in ear skin of symptomatic and asymptomatic Leishmania (Leishmania) chagasi infected dogs |
| title_full_unstemmed | Inflammatory response, parasite load and AgNOR expression in ear skin of symptomatic and asymptomatic Leishmania (Leishmania) chagasi infected dogs |
| title_short | Inflammatory response, parasite load and AgNOR expression in ear skin of symptomatic and asymptomatic Leishmania (Leishmania) chagasi infected dogs |
| title_sort | inflammatory response parasite load and agnor expression in ear skin of symptomatic and asymptomatic leishmania leishmania chagasi infected dogs |
| topic | visceral leishmaniasis inflammation parasites Leishmania chagasi |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300011 |
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