Potential Effect of Human Platelet Lysate on in vitro Expansion of Human Corneal Endothelial Cells Compared with Y-27632 ROCK Inhibitor
Abstract Purpose: Corneal endothelial cell (CEC) therapy can be used as a promising therapeutic option for patients with various corneal endothelial dysfunctions. In this study, we compared the proliferative effect of human platelet lysate (HPL), as a xeno-free medium supplement, with Y-27632 Rho/rh...
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| Format: | Article |
| Language: | English |
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Knowledge E
2021-07-01
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| Series: | Journal of Ophthalmic & Vision Research |
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| Online Access: | https://doi.org/10.18502/jovr.v16i3.9431 |
| _version_ | 1811255514060816384 |
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| author | Mohammad Amir Mishan Sahar Balagholi Tahereh Chamani Sepehr Feizi Zahra-Soheila Soheili Mozhgan Rezaei Kanavi |
| author_facet | Mohammad Amir Mishan Sahar Balagholi Tahereh Chamani Sepehr Feizi Zahra-Soheila Soheili Mozhgan Rezaei Kanavi |
| author_sort | Mohammad Amir Mishan |
| collection | DOAJ |
| description | Abstract Purpose: Corneal endothelial cell (CEC) therapy can be used as a promising therapeutic option for patients with various corneal endothelial dysfunctions. In this study, we compared the proliferative effect of human platelet lysate (HPL), as a xeno-free medium supplement, with Y-27632 Rho/rho-associated protein kinase (ROCK) inhibitor, as a well-known proliferative and adhesive agent for CECs, and fetal bovine serum (FBS) as the control, in the culture medium of human corneal endothelial cells (HCECs). Methods: We isolated HCECs from human donors and treated the cells as three different treatment groups including 20% HPL only, 10 μM Y-27632 ROCK inhibitor, combination of 20% HPL and 10 μM Y-27632 ROCK inhibitor, and 20% FBS as the control group. ELISA cell proliferation assay and cell counting was performed on the treated cells. Finally, HCECs were characterized by morphology and immunocytochemistry (ICC). Results: There was no significant proliferative effect of HPL on cell proliferation compared with the cells treated with Y-27632 ROCK inhibitor or the combination of HPL and Y-27632 ROCK inhibitor, but all the respected treatments had significant inducible effect on cell proliferation as compared with FBS-treated cells. The cells grown in all three treatment groups exhibited CEC morphology. Also, there was a higher expression of Na+/K+-ATPase and ZO-1, as CEC characteristic markers, in the culture of HCECs treated with HPL as compared with FBS. Conclusion: HPL offers a xeno - free and affordable medium supplement for CEC expansion that can be used in clinical applications. |
| first_indexed | 2024-04-12T17:25:57Z |
| format | Article |
| id | doaj.art-025c1fe3782f48699294e3c64477c6f3 |
| institution | Directory Open Access Journal |
| issn | 2008-2010 2008-322X |
| language | English |
| last_indexed | 2024-04-12T17:25:57Z |
| publishDate | 2021-07-01 |
| publisher | Knowledge E |
| record_format | Article |
| series | Journal of Ophthalmic & Vision Research |
| spelling | doaj.art-025c1fe3782f48699294e3c64477c6f32022-12-22T03:23:18ZengKnowledge EJournal of Ophthalmic & Vision Research2008-20102008-322X2021-07-0116334935610.18502/jovr.v16i3.9431jovr.v16i3.9431Potential Effect of Human Platelet Lysate on in vitro Expansion of Human Corneal Endothelial Cells Compared with Y-27632 ROCK InhibitorMohammad Amir Mishan0Sahar Balagholi1Tahereh Chamani2Sepehr Feizi3Zahra-Soheila Soheili4Mozhgan Rezaei Kanavi5 Ocular Tissue Engineering Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Central Eye Bank of Iran, Tehran, Iran Ophthalmic Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Ocular Tissue Engineering Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, IranAbstract Purpose: Corneal endothelial cell (CEC) therapy can be used as a promising therapeutic option for patients with various corneal endothelial dysfunctions. In this study, we compared the proliferative effect of human platelet lysate (HPL), as a xeno-free medium supplement, with Y-27632 Rho/rho-associated protein kinase (ROCK) inhibitor, as a well-known proliferative and adhesive agent for CECs, and fetal bovine serum (FBS) as the control, in the culture medium of human corneal endothelial cells (HCECs). Methods: We isolated HCECs from human donors and treated the cells as three different treatment groups including 20% HPL only, 10 μM Y-27632 ROCK inhibitor, combination of 20% HPL and 10 μM Y-27632 ROCK inhibitor, and 20% FBS as the control group. ELISA cell proliferation assay and cell counting was performed on the treated cells. Finally, HCECs were characterized by morphology and immunocytochemistry (ICC). Results: There was no significant proliferative effect of HPL on cell proliferation compared with the cells treated with Y-27632 ROCK inhibitor or the combination of HPL and Y-27632 ROCK inhibitor, but all the respected treatments had significant inducible effect on cell proliferation as compared with FBS-treated cells. The cells grown in all three treatment groups exhibited CEC morphology. Also, there was a higher expression of Na+/K+-ATPase and ZO-1, as CEC characteristic markers, in the culture of HCECs treated with HPL as compared with FBS. Conclusion: HPL offers a xeno - free and affordable medium supplement for CEC expansion that can be used in clinical applications.https://doi.org/10.18502/jovr.v16i3.9431cell proliferationcorneal endothelial cellshuman platelet lysaterock inhibitor |
| spellingShingle | Mohammad Amir Mishan Sahar Balagholi Tahereh Chamani Sepehr Feizi Zahra-Soheila Soheili Mozhgan Rezaei Kanavi Potential Effect of Human Platelet Lysate on in vitro Expansion of Human Corneal Endothelial Cells Compared with Y-27632 ROCK Inhibitor Journal of Ophthalmic & Vision Research cell proliferation corneal endothelial cells human platelet lysate rock inhibitor |
| title | Potential Effect of Human Platelet Lysate on in vitro Expansion of Human Corneal Endothelial Cells Compared with Y-27632 ROCK Inhibitor |
| title_full | Potential Effect of Human Platelet Lysate on in vitro Expansion of Human Corneal Endothelial Cells Compared with Y-27632 ROCK Inhibitor |
| title_fullStr | Potential Effect of Human Platelet Lysate on in vitro Expansion of Human Corneal Endothelial Cells Compared with Y-27632 ROCK Inhibitor |
| title_full_unstemmed | Potential Effect of Human Platelet Lysate on in vitro Expansion of Human Corneal Endothelial Cells Compared with Y-27632 ROCK Inhibitor |
| title_short | Potential Effect of Human Platelet Lysate on in vitro Expansion of Human Corneal Endothelial Cells Compared with Y-27632 ROCK Inhibitor |
| title_sort | potential effect of human platelet lysate on in vitro expansion of human corneal endothelial cells compared with y 27632 rock inhibitor |
| topic | cell proliferation corneal endothelial cells human platelet lysate rock inhibitor |
| url | https://doi.org/10.18502/jovr.v16i3.9431 |
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