Detection and characterisation of rabbit haemorrhagic disease virus strains circulating in Egypt
Rabbit haemorrhagic disease (RHD) is a highly virulent viral disease of Oryctolagus cuniculus which threatens the rabbit population in Egypt and worldwide. The etiological agent is the rabbit haemor-rhagic disease virus (RHDV), a member of the family Caliciviridae. The aim of this study was to ident...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria
2019-12-01
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| Series: | Bulgarian Journal of Veterinary Medicine |
| Subjects: | |
| Online Access: | http://tru.uni-sz.bg/bjvm/BJVM%20December%202019%20p.409-418.pdf |
| _version_ | 1818382375184236544 |
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| author | A. F. Magouz E. A. Elsayed A. Y. Metwally |
| author_facet | A. F. Magouz E. A. Elsayed A. Y. Metwally |
| author_sort | A. F. Magouz |
| collection | DOAJ |
| description | Rabbit haemorrhagic disease (RHD) is a highly virulent viral disease of Oryctolagus cuniculus which threatens the rabbit population in Egypt and worldwide. The etiological agent is the rabbit haemor-rhagic disease virus (RHDV), a member of the family Caliciviridae. The aim of this study was to identify the possible evolutionary changes of the currently circulating RHDV in Egypt. Twenty sus-pected cases were collected from outbreaks that occurred in non-vaccinated rabbit flocks during 2015 and 2016. Liver homogenate samples were investigated for detection and identification of circulating RHDV using haemagglutination (HA) and reverse transcriptase polymerase chain reaction. Further characterisation of selected five viral strains was performed by nucleotide sequencing of VP60 gene. All twenty tested samples were haemagglutinin positive and VP60 gene was amplified. Based on nucleotide sequence analysis, four isolates were identified as classical RHDV strains, while one iso-late was assigned as RHDVa variant strain but with the same HA pattern. The new RHDVb variant was not identified. |
| first_indexed | 2024-12-14T02:49:28Z |
| format | Article |
| id | doaj.art-025c36fa9fde4e55bd7ed7ac94ae6a86 |
| institution | Directory Open Access Journal |
| issn | 1311-1477 1313-3543 |
| language | English |
| last_indexed | 2024-12-14T02:49:28Z |
| publishDate | 2019-12-01 |
| publisher | Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria |
| record_format | Article |
| series | Bulgarian Journal of Veterinary Medicine |
| spelling | doaj.art-025c36fa9fde4e55bd7ed7ac94ae6a862022-12-21T23:19:47ZengFaculty of Veterinary Medicine, Trakia University, Stara Zagora, BulgariaBulgarian Journal of Veterinary Medicine1311-14771313-35432019-12-0122440941810.15547/bjvm.2085Detection and characterisation of rabbit haemorrhagic disease virus strains circulating in EgyptA. F. Magouz0E. A. Elsayed1A. Y. Metwally2Faculty of Veterinary Medicine, Kafrelsheikh University, EgyptFaculty of Veterinary Medicine, Kafrelsheikh University, EgyptDepartment of Poultry Diseases, Animal Health Research Institute, Kafrelsheikh, EgyptRabbit haemorrhagic disease (RHD) is a highly virulent viral disease of Oryctolagus cuniculus which threatens the rabbit population in Egypt and worldwide. The etiological agent is the rabbit haemor-rhagic disease virus (RHDV), a member of the family Caliciviridae. The aim of this study was to identify the possible evolutionary changes of the currently circulating RHDV in Egypt. Twenty sus-pected cases were collected from outbreaks that occurred in non-vaccinated rabbit flocks during 2015 and 2016. Liver homogenate samples were investigated for detection and identification of circulating RHDV using haemagglutination (HA) and reverse transcriptase polymerase chain reaction. Further characterisation of selected five viral strains was performed by nucleotide sequencing of VP60 gene. All twenty tested samples were haemagglutinin positive and VP60 gene was amplified. Based on nucleotide sequence analysis, four isolates were identified as classical RHDV strains, while one iso-late was assigned as RHDVa variant strain but with the same HA pattern. The new RHDVb variant was not identified.http://tru.uni-sz.bg/bjvm/BJVM%20December%202019%20p.409-418.pdfphylogenetic analysisrhdvrt-pcrvp60 gene |
| spellingShingle | A. F. Magouz E. A. Elsayed A. Y. Metwally Detection and characterisation of rabbit haemorrhagic disease virus strains circulating in Egypt Bulgarian Journal of Veterinary Medicine phylogenetic analysis rhdv rt-pcr vp60 gene |
| title | Detection and characterisation of rabbit haemorrhagic disease virus strains circulating in Egypt |
| title_full | Detection and characterisation of rabbit haemorrhagic disease virus strains circulating in Egypt |
| title_fullStr | Detection and characterisation of rabbit haemorrhagic disease virus strains circulating in Egypt |
| title_full_unstemmed | Detection and characterisation of rabbit haemorrhagic disease virus strains circulating in Egypt |
| title_short | Detection and characterisation of rabbit haemorrhagic disease virus strains circulating in Egypt |
| title_sort | detection and characterisation of rabbit haemorrhagic disease virus strains circulating in egypt |
| topic | phylogenetic analysis rhdv rt-pcr vp60 gene |
| url | http://tru.uni-sz.bg/bjvm/BJVM%20December%202019%20p.409-418.pdf |
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