Immunohistochemical Study of Glucose Transporter GLUT-5 in Duodenal Epithelium in Norm and in T-2 Mycotoxicosis
Although patterns of glucose transporter expression and notes about diseases leading to adaptive changes in intestinal fructose transport have been well-characterized, the connection between infection and fructose transportation has been lightly investigated. Up to now only few studies on GLUT-5 exp...
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MDPI AG
2020-06-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/7/849 |
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| author | Piret Hussar Florina Popovska-Percinic Katerina Blagoevska Tõnu Järveots Ilmārs Dūrītis |
| author_facet | Piret Hussar Florina Popovska-Percinic Katerina Blagoevska Tõnu Järveots Ilmārs Dūrītis |
| author_sort | Piret Hussar |
| collection | DOAJ |
| description | Although patterns of glucose transporter expression and notes about diseases leading to adaptive changes in intestinal fructose transport have been well-characterized, the connection between infection and fructose transportation has been lightly investigated. Up to now only few studies on GLUT-5 expression and function under pathological conditions in bird intestines have been carried out. The aim of our current research was to immunolocalize GLUT-5 in chicken duodenal epithelium in norm and during T-2 mycotoxicosis. Material from chicken <i>(Gallus gallus domesticus)</i> duodenum was collected from twelve seven-day-old female broilers, divided into control group and broilers with T-2 mycotoxicosis. The material was fixed with 10% formalin and thereafter embedded into paraffin; slices 7 μm in thickness were cut, followed by immunohistochemical staining, according to the manufacturers guidelines (IHC kit, Abcam, UK) using polyclonal primary antibody Rabbit anti-GLUT-5. Our study revealed the strong expression of GLUT-5 in the apical parts of the duodenal epithelial cells in the control group chickens and weak staining for GLUT-5 in the intestinal epithelium in the T-2 mycotoxicosis group. Our results confirmed decreased the expression of GLUT-5 in the duodenal epithelium during T-2 mycotoxicosis. |
| first_indexed | 2024-03-10T18:48:59Z |
| format | Article |
| id | doaj.art-025f4673f8fe4f02b8f60ad31ca7d65b |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T18:48:59Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-025f4673f8fe4f02b8f60ad31ca7d65b2023-11-20T05:17:18ZengMDPI AGFoods2304-81582020-06-019784910.3390/foods9070849Immunohistochemical Study of Glucose Transporter GLUT-5 in Duodenal Epithelium in Norm and in T-2 MycotoxicosisPiret Hussar0Florina Popovska-Percinic1Katerina Blagoevska2Tõnu Järveots3Ilmārs Dūrītis4Faculty of Medicine, University of Tartu, Ravila 19, 50411 Tartu, EstoniaFaculty of Veterinary Medicine, Ss.Cyril & Methodius University in Skopje, 1000 Skopje, North MacedoniaLaboratory for Molecular Food Analyses and Genetically Modified Organism, Food Institute, Faculty of Veterinary Medicine, 1000 Skopje, North MacedoniaInstitute of Veterinary Medicine and Animal Sciences, Estonian University of Life Sciences, 51006 Tartu, EstoniaFaculty of Veterinary Medicine, Latvian University of Agriculture, LV 3004 Jelgava, LatviaAlthough patterns of glucose transporter expression and notes about diseases leading to adaptive changes in intestinal fructose transport have been well-characterized, the connection between infection and fructose transportation has been lightly investigated. Up to now only few studies on GLUT-5 expression and function under pathological conditions in bird intestines have been carried out. The aim of our current research was to immunolocalize GLUT-5 in chicken duodenal epithelium in norm and during T-2 mycotoxicosis. Material from chicken <i>(Gallus gallus domesticus)</i> duodenum was collected from twelve seven-day-old female broilers, divided into control group and broilers with T-2 mycotoxicosis. The material was fixed with 10% formalin and thereafter embedded into paraffin; slices 7 μm in thickness were cut, followed by immunohistochemical staining, according to the manufacturers guidelines (IHC kit, Abcam, UK) using polyclonal primary antibody Rabbit anti-GLUT-5. Our study revealed the strong expression of GLUT-5 in the apical parts of the duodenal epithelial cells in the control group chickens and weak staining for GLUT-5 in the intestinal epithelium in the T-2 mycotoxicosis group. Our results confirmed decreased the expression of GLUT-5 in the duodenal epithelium during T-2 mycotoxicosis.https://www.mdpi.com/2304-8158/9/7/849GLUT-5duodenal epitheliumT-2 mycotoxicosis |
| spellingShingle | Piret Hussar Florina Popovska-Percinic Katerina Blagoevska Tõnu Järveots Ilmārs Dūrītis Immunohistochemical Study of Glucose Transporter GLUT-5 in Duodenal Epithelium in Norm and in T-2 Mycotoxicosis Foods GLUT-5 duodenal epithelium T-2 mycotoxicosis |
| title | Immunohistochemical Study of Glucose Transporter GLUT-5 in Duodenal Epithelium in Norm and in T-2 Mycotoxicosis |
| title_full | Immunohistochemical Study of Glucose Transporter GLUT-5 in Duodenal Epithelium in Norm and in T-2 Mycotoxicosis |
| title_fullStr | Immunohistochemical Study of Glucose Transporter GLUT-5 in Duodenal Epithelium in Norm and in T-2 Mycotoxicosis |
| title_full_unstemmed | Immunohistochemical Study of Glucose Transporter GLUT-5 in Duodenal Epithelium in Norm and in T-2 Mycotoxicosis |
| title_short | Immunohistochemical Study of Glucose Transporter GLUT-5 in Duodenal Epithelium in Norm and in T-2 Mycotoxicosis |
| title_sort | immunohistochemical study of glucose transporter glut 5 in duodenal epithelium in norm and in t 2 mycotoxicosis |
| topic | GLUT-5 duodenal epithelium T-2 mycotoxicosis |
| url | https://www.mdpi.com/2304-8158/9/7/849 |
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