Identification of genomic regions associated with shoot fly resistance in maize and their syntenic relationships in the sorghum genome.
Shoot fly (Atherigona naqvii) is one of the major insects affecting spring maize in North India and can cause yield loss up to 60 per cent. The genetics of insect resistance is complex as influenced by genotypic background, insect population and climatic conditions. Therefore, quantitative trait loc...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0234335 |
| _version_ | 1819147663968305152 |
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| author | Yogesh Vikal Arshpreet Kaur Jawala Jindal Kirandeep Kaur Dharminder Pathak Tosh Garg Ashanpreet Singh Princepal Singh Inderjit Yadav |
| author_facet | Yogesh Vikal Arshpreet Kaur Jawala Jindal Kirandeep Kaur Dharminder Pathak Tosh Garg Ashanpreet Singh Princepal Singh Inderjit Yadav |
| author_sort | Yogesh Vikal |
| collection | DOAJ |
| description | Shoot fly (Atherigona naqvii) is one of the major insects affecting spring maize in North India and can cause yield loss up to 60 per cent. The genetics of insect resistance is complex as influenced by genotypic background, insect population and climatic conditions. Therefore, quantitative trait loci (QTL) mapping is a highly effective approach for studying genetically complex forms of insect resistance. The objective of the present study was to dissect the genetic basis of resistance and identification of genomic regions associated with shoot fly resistance. A total of 107 F2 population derived from the cross CM143 (resistant) x CM144 (susceptible) was genotyped with 120 SSR markers. Phenotypic data were recorded on replicated F2:3 progenies for various component traits imparting resistance to shoot fly at different time intervals. Resistance to shoot fly was observed to be under polygenic control as evidenced by the identification of 19 putative QTLs governed by overdominance to partial dominance and additive gene actions. The major QTLs conditioning shoot fly resistance viz., qDH9.1 (deadheart) and qEC9.1 (oviposition) explaining 15.03 and 18.89 per cent phenotypic variance, respectively were colocalized on chromosome 9. These QTLs are syntenic to regions of chromosome 10 of sorghum which were also accounted for deadheart and oviposition suggesting that the same gene block may be responsible for shoot fly resistance. The candidate genes such as cysteine protease, subtilisin-chymotrypsin inhibitor, cytochrome P450 involved in synthesis of alleochemicals, receptor kinases, glossy15 and ubiquitin-proteasome degradation pathway were identified within the predicted QTL regions. This is the first reported mapping of QTLs conferring resistance to shoot fly in maize, and the markers identified here will be a valuable resource for developing elite maize cultivars with resistance to shoot fly. |
| first_indexed | 2024-12-22T13:33:24Z |
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| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T13:33:24Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-026260865d834801a3f3f522b85e19c22022-12-21T18:24:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01156e023433510.1371/journal.pone.0234335Identification of genomic regions associated with shoot fly resistance in maize and their syntenic relationships in the sorghum genome.Yogesh VikalArshpreet KaurJawala JindalKirandeep KaurDharminder PathakTosh GargAshanpreet SinghPrincepal SinghInderjit YadavShoot fly (Atherigona naqvii) is one of the major insects affecting spring maize in North India and can cause yield loss up to 60 per cent. The genetics of insect resistance is complex as influenced by genotypic background, insect population and climatic conditions. Therefore, quantitative trait loci (QTL) mapping is a highly effective approach for studying genetically complex forms of insect resistance. The objective of the present study was to dissect the genetic basis of resistance and identification of genomic regions associated with shoot fly resistance. A total of 107 F2 population derived from the cross CM143 (resistant) x CM144 (susceptible) was genotyped with 120 SSR markers. Phenotypic data were recorded on replicated F2:3 progenies for various component traits imparting resistance to shoot fly at different time intervals. Resistance to shoot fly was observed to be under polygenic control as evidenced by the identification of 19 putative QTLs governed by overdominance to partial dominance and additive gene actions. The major QTLs conditioning shoot fly resistance viz., qDH9.1 (deadheart) and qEC9.1 (oviposition) explaining 15.03 and 18.89 per cent phenotypic variance, respectively were colocalized on chromosome 9. These QTLs are syntenic to regions of chromosome 10 of sorghum which were also accounted for deadheart and oviposition suggesting that the same gene block may be responsible for shoot fly resistance. The candidate genes such as cysteine protease, subtilisin-chymotrypsin inhibitor, cytochrome P450 involved in synthesis of alleochemicals, receptor kinases, glossy15 and ubiquitin-proteasome degradation pathway were identified within the predicted QTL regions. This is the first reported mapping of QTLs conferring resistance to shoot fly in maize, and the markers identified here will be a valuable resource for developing elite maize cultivars with resistance to shoot fly.https://doi.org/10.1371/journal.pone.0234335 |
| spellingShingle | Yogesh Vikal Arshpreet Kaur Jawala Jindal Kirandeep Kaur Dharminder Pathak Tosh Garg Ashanpreet Singh Princepal Singh Inderjit Yadav Identification of genomic regions associated with shoot fly resistance in maize and their syntenic relationships in the sorghum genome. PLoS ONE |
| title | Identification of genomic regions associated with shoot fly resistance in maize and their syntenic relationships in the sorghum genome. |
| title_full | Identification of genomic regions associated with shoot fly resistance in maize and their syntenic relationships in the sorghum genome. |
| title_fullStr | Identification of genomic regions associated with shoot fly resistance in maize and their syntenic relationships in the sorghum genome. |
| title_full_unstemmed | Identification of genomic regions associated with shoot fly resistance in maize and their syntenic relationships in the sorghum genome. |
| title_short | Identification of genomic regions associated with shoot fly resistance in maize and their syntenic relationships in the sorghum genome. |
| title_sort | identification of genomic regions associated with shoot fly resistance in maize and their syntenic relationships in the sorghum genome |
| url | https://doi.org/10.1371/journal.pone.0234335 |
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