Identification and enzymatic characterization of the yeasts isolated from Erzincan tulum cheese

In this study, 146 yeast isolates were obtained from 45 Erzincan tulum cheese samples. By using API ID 32C test system and some complementary morphological, physiological and biochemica tests, 121 of the isolates could be identified at species level, while 12 of them were identified at genus level....

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Main Authors: S. Karasu-Yalcin, S. Senses-Ergul, Z. Yesim Ozbas
Format: Article
Language:English
Published: Croatian Dairy Union 2012-03-01
Series:Mljekarstvo
Subjects:
Online Access:http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=116555
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author S. Karasu-Yalcin
S. Senses-Ergul
Z. Yesim Ozbas
author_facet S. Karasu-Yalcin
S. Senses-Ergul
Z. Yesim Ozbas
author_sort S. Karasu-Yalcin
collection DOAJ
description In this study, 146 yeast isolates were obtained from 45 Erzincan tulum cheese samples. By using API ID 32C test system and some complementary morphological, physiological and biochemica tests, 121 of the isolates could be identified at species level, while 12 of them were identified at genus level. The identified yeast isolates belonged to six different genera which were Candida, Geotrichum, Kluyveromyces, Pichia, Saccharomyces and Zygosaccharomyces. The most aboundant species was C. lambica, followed by C. zeylanoides, C. famata var. famata, G. candidum and C. kefyr. Enzymatic characterization of the strains was determined by using API-ZYM test system. All of the isolates had leucin arylamidase activity. Eight strains belonging to S. cerevisiae, Z. mellis, G. candidum and P. fermentans were found to have high leucin arylamidase activities. Most of the isolates had β-galactosidase, acid phosphatase and esterase lipase (C8) activities. Eight investigated C. lambica strains had high acid phosphatase activities. Such enzymatic properties of investigated yeast isolates could be fundamental factor for their application as starter culture candidates in production of Erzincan tulum cheese. It was demonstrated that the strain C. lambica T103 had superior enzymatic characteristics with the potential to be used in further technological investigations as an adjunct starter.
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spelling doaj.art-0262dc0d6ffe43b388e50828571ae7802022-12-22T00:43:37ZengCroatian Dairy UnionMljekarstvo0026-704X1846-40252012-03-016215361Identification and enzymatic characterization of the yeasts isolated from Erzincan tulum cheeseS. Karasu-YalcinS. Senses-ErgulZ. Yesim OzbasIn this study, 146 yeast isolates were obtained from 45 Erzincan tulum cheese samples. By using API ID 32C test system and some complementary morphological, physiological and biochemica tests, 121 of the isolates could be identified at species level, while 12 of them were identified at genus level. The identified yeast isolates belonged to six different genera which were Candida, Geotrichum, Kluyveromyces, Pichia, Saccharomyces and Zygosaccharomyces. The most aboundant species was C. lambica, followed by C. zeylanoides, C. famata var. famata, G. candidum and C. kefyr. Enzymatic characterization of the strains was determined by using API-ZYM test system. All of the isolates had leucin arylamidase activity. Eight strains belonging to S. cerevisiae, Z. mellis, G. candidum and P. fermentans were found to have high leucin arylamidase activities. Most of the isolates had β-galactosidase, acid phosphatase and esterase lipase (C8) activities. Eight investigated C. lambica strains had high acid phosphatase activities. Such enzymatic properties of investigated yeast isolates could be fundamental factor for their application as starter culture candidates in production of Erzincan tulum cheese. It was demonstrated that the strain C. lambica T103 had superior enzymatic characteristics with the potential to be used in further technological investigations as an adjunct starter.http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=116555Erzincan tulum cheeseyeastisolationidentificationenzymatic activity
spellingShingle S. Karasu-Yalcin
S. Senses-Ergul
Z. Yesim Ozbas
Identification and enzymatic characterization of the yeasts isolated from Erzincan tulum cheese
Mljekarstvo
Erzincan tulum cheese
yeast
isolation
identification
enzymatic activity
title Identification and enzymatic characterization of the yeasts isolated from Erzincan tulum cheese
title_full Identification and enzymatic characterization of the yeasts isolated from Erzincan tulum cheese
title_fullStr Identification and enzymatic characterization of the yeasts isolated from Erzincan tulum cheese
title_full_unstemmed Identification and enzymatic characterization of the yeasts isolated from Erzincan tulum cheese
title_short Identification and enzymatic characterization of the yeasts isolated from Erzincan tulum cheese
title_sort identification and enzymatic characterization of the yeasts isolated from erzincan tulum cheese
topic Erzincan tulum cheese
yeast
isolation
identification
enzymatic activity
url http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=116555
work_keys_str_mv AT skarasuyalcin identificationandenzymaticcharacterizationoftheyeastsisolatedfromerzincantulumcheese
AT ssensesergul identificationandenzymaticcharacterizationoftheyeastsisolatedfromerzincantulumcheese
AT zyesimozbas identificationandenzymaticcharacterizationoftheyeastsisolatedfromerzincantulumcheese