Evaluation of the Expression of CCR5 and CX3CR1 Receptors and Correlation with the Functionality of T Cells in Women infected with ZIKV during Pregnancy
There have been reports of neurological abnormalities associated with the Zika virus (ZIKV), such as congenital Zika syndrome (CZS) in children born to mothers infected during pregnancy. We investigated how the immune response to ZIKV during pregnancy is primed and conduct a thorough evaluation of t...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-01-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/13/2/191 |
| _version_ | 1797406547119177728 |
|---|---|
| author | Débora Familiar-Macedo Iury Amancio Paiva Jessica Badolato-Corrêa da Silva Fabiana Rabe de Carvalho Helver Gonçalves Dias Alex Pauvolid-Corrêa Caroline Fernandes dos Santos Mariana Gandini Andréa Alice Silva Silvia Maria Baeta Cavalcanti Solange Artimos de Oliveira Renata Artimos de Oliveira Vianna Elzinandes Leal de Azeredo Alba Grifoni Alessandro Sette Daniela Weiskopf Claudete Aparecida Araújo Cardoso Luzia Maria de-Oliveira-Pinto |
| author_facet | Débora Familiar-Macedo Iury Amancio Paiva Jessica Badolato-Corrêa da Silva Fabiana Rabe de Carvalho Helver Gonçalves Dias Alex Pauvolid-Corrêa Caroline Fernandes dos Santos Mariana Gandini Andréa Alice Silva Silvia Maria Baeta Cavalcanti Solange Artimos de Oliveira Renata Artimos de Oliveira Vianna Elzinandes Leal de Azeredo Alba Grifoni Alessandro Sette Daniela Weiskopf Claudete Aparecida Araújo Cardoso Luzia Maria de-Oliveira-Pinto |
| author_sort | Débora Familiar-Macedo |
| collection | DOAJ |
| description | There have been reports of neurological abnormalities associated with the Zika virus (ZIKV), such as congenital Zika syndrome (CZS) in children born to mothers infected during pregnancy. We investigated how the immune response to ZIKV during pregnancy is primed and conduct a thorough evaluation of the inflammatory and cytotoxic profiles as well as the expression of CCR5 and CX3CR1. We compared the reactivity of T cells to ZIKV peptides in convalescent mothers infected during pregnancy. The child’s clinical outcome (i.e., born with or without CZS) was taken to be the variable. The cells were stimulated in vitro with ZIKV peptides and evaluated using the ELISPOT and flow cytometry assays. After in vitro stimulation with ZIKV peptides, we observed a tendency toward a higher Interferon gamma (IFN-γ)-producing T cell responses in mothers who had asymptomatic children and a higher CD107a expression in T cells in mothers who had children with CZS. We found a higher frequency of T cells expressing CD107a+ and co-expressing CX3CR1+CCR5+, which is much clearer in the T cells of mothers who had CZS children. We suggest that this differential profile influenced the clinical outcome of babies. These data need to be further investigated, including the evaluation of other ZIKV peptides and markers and functional assays. |
| first_indexed | 2024-03-09T03:28:00Z |
| format | Article |
| id | doaj.art-026357e1c423480aa1c4324bedf727dc |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T03:28:00Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-026357e1c423480aa1c4324bedf727dc2023-12-03T14:59:06ZengMDPI AGViruses1999-49152021-01-0113219110.3390/v13020191Evaluation of the Expression of CCR5 and CX3CR1 Receptors and Correlation with the Functionality of T Cells in Women infected with ZIKV during PregnancyDébora Familiar-Macedo0Iury Amancio Paiva1Jessica Badolato-Corrêa da Silva2Fabiana Rabe de Carvalho3Helver Gonçalves Dias4Alex Pauvolid-Corrêa5Caroline Fernandes dos Santos6Mariana Gandini7Andréa Alice Silva8Silvia Maria Baeta Cavalcanti9Solange Artimos de Oliveira10Renata Artimos de Oliveira Vianna11Elzinandes Leal de Azeredo12Alba Grifoni13Alessandro Sette14Daniela Weiskopf15Claudete Aparecida Araújo Cardoso16Luzia Maria de-Oliveira-Pinto17Laboratory of Viral Immunology, Fundação Oswaldo Cruz, Rio de Janeiro 1040-900, BrazilLaboratory of Viral Immunology, Fundação Oswaldo Cruz, Rio de Janeiro 1040-900, BrazilLaboratory of Viral Immunology, Fundação Oswaldo Cruz, Rio de Janeiro 1040-900, BrazilMultiuser Laboratory for Research in Nephrology and Medical Science, School of Medicine, Universidade Federal Fluminense, Niterói, Rio de Janeiro 24 033-900, BrazilLaboratory of Viral Immunology, Fundação Oswaldo Cruz, Rio de Janeiro 1040-900, BrazilDepartment of Veterinary Integrative Biosciences, Texas A&M University, Texas, TX 77843, USALaboratory of Viral Immunology, Fundação Oswaldo Cruz, Rio de Janeiro 1040-900, BrazilLaboratory of Cellular Microbiology, Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, BrazilMultiuser Laboratory for Research in Nephrology and Medical Science, School of Medicine, Universidade Federal Fluminense, Niterói, Rio de Janeiro 24 033-900, BrazilLaboratory of Virological Diagnosis, Biomedical Institute, Universidade Federal Fluminense, Niterói, Rio de Janeiro 24 110-130, BrazilDepartment of Maternal and Child, School of Medicine, Universidade Federal Fluminense, Niterói, Rio de Janeiro 24 033-900, BrazilDepartment of Maternal and Child, School of Medicine, Universidade Federal Fluminense, Niterói, Rio de Janeiro 24 033-900, BrazilLaboratory of Viral Immunology, Fundação Oswaldo Cruz, Rio de Janeiro 1040-900, BrazilCenter for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology (LJI), La Jolla, CA 92037, USACenter for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology (LJI), La Jolla, CA 92037, USACenter for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology (LJI), La Jolla, CA 92037, USAMultiuser Laboratory for Research in Nephrology and Medical Science, School of Medicine, Universidade Federal Fluminense, Niterói, Rio de Janeiro 24 033-900, BrazilLaboratory of Viral Immunology, Fundação Oswaldo Cruz, Rio de Janeiro 1040-900, BrazilThere have been reports of neurological abnormalities associated with the Zika virus (ZIKV), such as congenital Zika syndrome (CZS) in children born to mothers infected during pregnancy. We investigated how the immune response to ZIKV during pregnancy is primed and conduct a thorough evaluation of the inflammatory and cytotoxic profiles as well as the expression of CCR5 and CX3CR1. We compared the reactivity of T cells to ZIKV peptides in convalescent mothers infected during pregnancy. The child’s clinical outcome (i.e., born with or without CZS) was taken to be the variable. The cells were stimulated in vitro with ZIKV peptides and evaluated using the ELISPOT and flow cytometry assays. After in vitro stimulation with ZIKV peptides, we observed a tendency toward a higher Interferon gamma (IFN-γ)-producing T cell responses in mothers who had asymptomatic children and a higher CD107a expression in T cells in mothers who had children with CZS. We found a higher frequency of T cells expressing CD107a+ and co-expressing CX3CR1+CCR5+, which is much clearer in the T cells of mothers who had CZS children. We suggest that this differential profile influenced the clinical outcome of babies. These data need to be further investigated, including the evaluation of other ZIKV peptides and markers and functional assays.https://www.mdpi.com/1999-4915/13/2/191ZIKVpregnancyT cellsinflammatory responsecytotoxic activitychemokine receptors |
| spellingShingle | Débora Familiar-Macedo Iury Amancio Paiva Jessica Badolato-Corrêa da Silva Fabiana Rabe de Carvalho Helver Gonçalves Dias Alex Pauvolid-Corrêa Caroline Fernandes dos Santos Mariana Gandini Andréa Alice Silva Silvia Maria Baeta Cavalcanti Solange Artimos de Oliveira Renata Artimos de Oliveira Vianna Elzinandes Leal de Azeredo Alba Grifoni Alessandro Sette Daniela Weiskopf Claudete Aparecida Araújo Cardoso Luzia Maria de-Oliveira-Pinto Evaluation of the Expression of CCR5 and CX3CR1 Receptors and Correlation with the Functionality of T Cells in Women infected with ZIKV during Pregnancy Viruses ZIKV pregnancy T cells inflammatory response cytotoxic activity chemokine receptors |
| title | Evaluation of the Expression of CCR5 and CX3CR1 Receptors and Correlation with the Functionality of T Cells in Women infected with ZIKV during Pregnancy |
| title_full | Evaluation of the Expression of CCR5 and CX3CR1 Receptors and Correlation with the Functionality of T Cells in Women infected with ZIKV during Pregnancy |
| title_fullStr | Evaluation of the Expression of CCR5 and CX3CR1 Receptors and Correlation with the Functionality of T Cells in Women infected with ZIKV during Pregnancy |
| title_full_unstemmed | Evaluation of the Expression of CCR5 and CX3CR1 Receptors and Correlation with the Functionality of T Cells in Women infected with ZIKV during Pregnancy |
| title_short | Evaluation of the Expression of CCR5 and CX3CR1 Receptors and Correlation with the Functionality of T Cells in Women infected with ZIKV during Pregnancy |
| title_sort | evaluation of the expression of ccr5 and cx3cr1 receptors and correlation with the functionality of t cells in women infected with zikv during pregnancy |
| topic | ZIKV pregnancy T cells inflammatory response cytotoxic activity chemokine receptors |
| url | https://www.mdpi.com/1999-4915/13/2/191 |
| work_keys_str_mv | AT deborafamiliarmacedo evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT iuryamanciopaiva evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT jessicabadolatocorreadasilva evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT fabianarabedecarvalho evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT helvergoncalvesdias evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT alexpauvolidcorrea evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT carolinefernandesdossantos evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT marianagandini evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT andreaalicesilva evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT silviamariabaetacavalcanti evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT solangeartimosdeoliveira evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT renataartimosdeoliveiravianna evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT elzinandeslealdeazeredo evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT albagrifoni evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT alessandrosette evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT danielaweiskopf evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT claudeteaparecidaaraujocardoso evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy AT luziamariadeoliveirapinto evaluationoftheexpressionofccr5andcx3cr1receptorsandcorrelationwiththefunctionalityoftcellsinwomeninfectedwithzikvduringpregnancy |