A candidate nanoparticle vaccine comprised of multiple epitopes of the African swine fever virus elicits a robust immune response
Abstract The African swine fever (ASF) pandemics pose a significant threat to the global swine industry, and the development of safe and effective vaccines is a daunting but necessary challenge. The level and persistence of immunity are very important for the effectiveness of the vaccine. Targeting...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-023-02210-9 |
| _version_ | 1797556689060233216 |
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| author | Jinxing Song Mengxiang Wang Lei Zhou Panpan Tian ZhuoYa Sun Junru Sun Xuannian Wang Guoqing Zhuang Dawei Jiang Yanan Wu Gaiping Zhang |
| author_facet | Jinxing Song Mengxiang Wang Lei Zhou Panpan Tian ZhuoYa Sun Junru Sun Xuannian Wang Guoqing Zhuang Dawei Jiang Yanan Wu Gaiping Zhang |
| author_sort | Jinxing Song |
| collection | DOAJ |
| description | Abstract The African swine fever (ASF) pandemics pose a significant threat to the global swine industry, and the development of safe and effective vaccines is a daunting but necessary challenge. The level and persistence of immunity are very important for the effectiveness of the vaccine. Targeting antigens to antigen presenting cells (APCs) can greatly enhance immunogenicity. In this study, we developed a self-assembled nano-ASFV vaccine candidate (NanoFVax) targeting DCs, by covalently coupling the self-assembled 24-mer ferritin with the dominant B and T cell epitopes of the highly immunogenic ASFV antigen (p72, CD2v, pB602L and p30) and fused with the chemokine receptor XCL1 (a DC targeting molecule) through the SpyTag/SpyCatcher protein ligase system. Compared to monomeric protein, the nanoparticle vaccines can induce a more robust T-cell response, and the high-level antibody response against ASFV can last for more than 231 days. Therefore, the NanoFVax is a novel and promising vaccine candidate for ASFV. Graphical Abstract |
| first_indexed | 2024-03-10T17:05:27Z |
| format | Article |
| id | doaj.art-0265e384dd1a478c9bafc16ac9899bb0 |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-03-10T17:05:27Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-0265e384dd1a478c9bafc16ac9899bb02023-11-20T10:48:21ZengBMCJournal of Nanobiotechnology1477-31552023-11-0121111810.1186/s12951-023-02210-9A candidate nanoparticle vaccine comprised of multiple epitopes of the African swine fever virus elicits a robust immune responseJinxing Song0Mengxiang Wang1Lei Zhou2Panpan Tian3ZhuoYa Sun4Junru Sun5Xuannian Wang6Guoqing Zhuang7Dawei Jiang8Yanan Wu9Gaiping Zhang10International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural UniversityInternational Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural UniversityInternational Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural UniversityInternational Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural UniversityInternational Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural UniversityInternational Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural UniversityInternational Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural UniversityInternational Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural UniversityInternational Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural UniversityInternational Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural UniversityInternational Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural UniversityAbstract The African swine fever (ASF) pandemics pose a significant threat to the global swine industry, and the development of safe and effective vaccines is a daunting but necessary challenge. The level and persistence of immunity are very important for the effectiveness of the vaccine. Targeting antigens to antigen presenting cells (APCs) can greatly enhance immunogenicity. In this study, we developed a self-assembled nano-ASFV vaccine candidate (NanoFVax) targeting DCs, by covalently coupling the self-assembled 24-mer ferritin with the dominant B and T cell epitopes of the highly immunogenic ASFV antigen (p72, CD2v, pB602L and p30) and fused with the chemokine receptor XCL1 (a DC targeting molecule) through the SpyTag/SpyCatcher protein ligase system. Compared to monomeric protein, the nanoparticle vaccines can induce a more robust T-cell response, and the high-level antibody response against ASFV can last for more than 231 days. Therefore, the NanoFVax is a novel and promising vaccine candidate for ASFV. Graphical Abstracthttps://doi.org/10.1186/s12951-023-02210-9African swine feverNanoparticlesXCL1NanoFVaxFerritinVaccine candidate |
| spellingShingle | Jinxing Song Mengxiang Wang Lei Zhou Panpan Tian ZhuoYa Sun Junru Sun Xuannian Wang Guoqing Zhuang Dawei Jiang Yanan Wu Gaiping Zhang A candidate nanoparticle vaccine comprised of multiple epitopes of the African swine fever virus elicits a robust immune response Journal of Nanobiotechnology African swine fever Nanoparticles XCL1 NanoFVax Ferritin Vaccine candidate |
| title | A candidate nanoparticle vaccine comprised of multiple epitopes of the African swine fever virus elicits a robust immune response |
| title_full | A candidate nanoparticle vaccine comprised of multiple epitopes of the African swine fever virus elicits a robust immune response |
| title_fullStr | A candidate nanoparticle vaccine comprised of multiple epitopes of the African swine fever virus elicits a robust immune response |
| title_full_unstemmed | A candidate nanoparticle vaccine comprised of multiple epitopes of the African swine fever virus elicits a robust immune response |
| title_short | A candidate nanoparticle vaccine comprised of multiple epitopes of the African swine fever virus elicits a robust immune response |
| title_sort | candidate nanoparticle vaccine comprised of multiple epitopes of the african swine fever virus elicits a robust immune response |
| topic | African swine fever Nanoparticles XCL1 NanoFVax Ferritin Vaccine candidate |
| url | https://doi.org/10.1186/s12951-023-02210-9 |
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