Continuous wavelet transform for solving the problem of minor components in quantitation of pharmaceuticals: a case study on the mixture of ibuprofen and phenylephrine with its degradation products
Abstract The presence of minor components represents a challenging problem in spectrophotometric analysis of pharmaceuticals. If one component has a low absorptivity or present in a low concentration compared to the other components, this will hinder its quantitation by spectrophotometric methods. C...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Chemistry |
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| Online Access: | https://doi.org/10.1186/s13065-023-01059-1 |
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| author | Said A. Hassan Reham A. Fekry Yasmin M. Fayez Khadiga M. Kelani |
| author_facet | Said A. Hassan Reham A. Fekry Yasmin M. Fayez Khadiga M. Kelani |
| author_sort | Said A. Hassan |
| collection | DOAJ |
| description | Abstract The presence of minor components represents a challenging problem in spectrophotometric analysis of pharmaceuticals. If one component has a low absorptivity or present in a low concentration compared to the other components, this will hinder its quantitation by spectrophotometric methods. Continuous Wavelet Transform (CWT) as a signal processing technique was utilized to figure out a solution to such a problem. A comparative study was established between traditional derivative spectrophotometry (Numerical Differentiation, ND) and CWT to indicate the advantages and limitations of each technique and possibility of solving the problem of minor components. A mixture of ibuprofen (IBU) and phenylephrine (PHE) with its degradation products forming a ternary mixture was used for comparing the two techniques. The two techniques were applied on raw spectral data and on ratio spectra data resulting in four methods, namely ND, CWT, Derivative Ratio-Zero Crossing (DRZC) and Continuous Wavelet Transform Ratio-Zero Crossing (CWTR-ZC) methods. By comparing the results in laboratory prepared mixtures, CWT technique showed advantages in analysis of mixtures with minor components than ND. The proposed methods were validated according to the ICH guideline Q2(R1), where their linearity was established with correlation coefficient ranging from 0.9995 to 0.9999. The linearity was in the range 3–40 μg/mL for PHE in all methods, while for IBU it was 20–180 and 30–180 μg/mL in CWT and ND methods, respectively. The CWT methods were applied for quantitative determination of the drugs in their dosage form showing the ability of the methods to quantitate minor components in pharmaceutical formulations. |
| first_indexed | 2024-03-09T15:30:47Z |
| format | Article |
| id | doaj.art-02692420a86f40f18482864a3d5a760e |
| institution | Directory Open Access Journal |
| issn | 2661-801X |
| language | English |
| last_indexed | 2024-03-09T15:30:47Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Chemistry |
| spelling | doaj.art-02692420a86f40f18482864a3d5a760e2023-11-26T12:13:41ZengBMCBMC Chemistry2661-801X2023-10-0117111410.1186/s13065-023-01059-1Continuous wavelet transform for solving the problem of minor components in quantitation of pharmaceuticals: a case study on the mixture of ibuprofen and phenylephrine with its degradation productsSaid A. Hassan0Reham A. Fekry1Yasmin M. Fayez2Khadiga M. Kelani3Analytical Chemistry Department, Faculty of Pharmacy, Cairo UniversityAnalytical Chemistry Department, Faculty of Pharmacy, Modern University for Technology and InformationAnalytical Chemistry Department, Faculty of Pharmacy, Cairo UniversityAnalytical Chemistry Department, Faculty of Pharmacy, Cairo UniversityAbstract The presence of minor components represents a challenging problem in spectrophotometric analysis of pharmaceuticals. If one component has a low absorptivity or present in a low concentration compared to the other components, this will hinder its quantitation by spectrophotometric methods. Continuous Wavelet Transform (CWT) as a signal processing technique was utilized to figure out a solution to such a problem. A comparative study was established between traditional derivative spectrophotometry (Numerical Differentiation, ND) and CWT to indicate the advantages and limitations of each technique and possibility of solving the problem of minor components. A mixture of ibuprofen (IBU) and phenylephrine (PHE) with its degradation products forming a ternary mixture was used for comparing the two techniques. The two techniques were applied on raw spectral data and on ratio spectra data resulting in four methods, namely ND, CWT, Derivative Ratio-Zero Crossing (DRZC) and Continuous Wavelet Transform Ratio-Zero Crossing (CWTR-ZC) methods. By comparing the results in laboratory prepared mixtures, CWT technique showed advantages in analysis of mixtures with minor components than ND. The proposed methods were validated according to the ICH guideline Q2(R1), where their linearity was established with correlation coefficient ranging from 0.9995 to 0.9999. The linearity was in the range 3–40 μg/mL for PHE in all methods, while for IBU it was 20–180 and 30–180 μg/mL in CWT and ND methods, respectively. The CWT methods were applied for quantitative determination of the drugs in their dosage form showing the ability of the methods to quantitate minor components in pharmaceutical formulations.https://doi.org/10.1186/s13065-023-01059-1Derivative ratio-zero crossingDerivative spectrophotometryIbuprofenMinor componentNumerical differentiationPhenylephrine |
| spellingShingle | Said A. Hassan Reham A. Fekry Yasmin M. Fayez Khadiga M. Kelani Continuous wavelet transform for solving the problem of minor components in quantitation of pharmaceuticals: a case study on the mixture of ibuprofen and phenylephrine with its degradation products BMC Chemistry Derivative ratio-zero crossing Derivative spectrophotometry Ibuprofen Minor component Numerical differentiation Phenylephrine |
| title | Continuous wavelet transform for solving the problem of minor components in quantitation of pharmaceuticals: a case study on the mixture of ibuprofen and phenylephrine with its degradation products |
| title_full | Continuous wavelet transform for solving the problem of minor components in quantitation of pharmaceuticals: a case study on the mixture of ibuprofen and phenylephrine with its degradation products |
| title_fullStr | Continuous wavelet transform for solving the problem of minor components in quantitation of pharmaceuticals: a case study on the mixture of ibuprofen and phenylephrine with its degradation products |
| title_full_unstemmed | Continuous wavelet transform for solving the problem of minor components in quantitation of pharmaceuticals: a case study on the mixture of ibuprofen and phenylephrine with its degradation products |
| title_short | Continuous wavelet transform for solving the problem of minor components in quantitation of pharmaceuticals: a case study on the mixture of ibuprofen and phenylephrine with its degradation products |
| title_sort | continuous wavelet transform for solving the problem of minor components in quantitation of pharmaceuticals a case study on the mixture of ibuprofen and phenylephrine with its degradation products |
| topic | Derivative ratio-zero crossing Derivative spectrophotometry Ibuprofen Minor component Numerical differentiation Phenylephrine |
| url | https://doi.org/10.1186/s13065-023-01059-1 |
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