Gut Microbiome and Its Cofactors Are Linked to Lipoprotein Distribution Profiles
Increasing evidence indicates that the gut microbiome (GM) plays an important role in dyslipidemia. To date, however, no in-depth characterization of the associations between GM with lipoproteins distributions (LPD) among adult individuals with diverse BMI has been conducted. To determine such assoc...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/10/11/2156 |
| _version_ | 1797467148268863488 |
|---|---|
| author | Josué L. Castro-Mejía Bekzod Khakimov Violetta Aru Mads V. Lind Eva Garne Petronela Paulová Elnaz Tavakkoli Lars H. Hansen Age K. Smilde Lars Holm Søren B. Engelsen Dennis S. Nielsen |
| author_facet | Josué L. Castro-Mejía Bekzod Khakimov Violetta Aru Mads V. Lind Eva Garne Petronela Paulová Elnaz Tavakkoli Lars H. Hansen Age K. Smilde Lars Holm Søren B. Engelsen Dennis S. Nielsen |
| author_sort | Josué L. Castro-Mejía |
| collection | DOAJ |
| description | Increasing evidence indicates that the gut microbiome (GM) plays an important role in dyslipidemia. To date, however, no in-depth characterization of the associations between GM with lipoproteins distributions (LPD) among adult individuals with diverse BMI has been conducted. To determine such associations, we studied blood-plasma LPD, fecal short-chain fatty acids (SCFA) and GM of 262 Danes aged 19–89 years. Stratification of LPD segregated subjects into three clusters displaying recommended levels of lipoproteins and explained by age and body-mass-index. Higher levels of HDL2a and HDL2b were associated with a higher abundance of <i>Ruminococcaceae</i> and <i>Christensenellaceae</i>. Increasing levels of total cholesterol and LDL-1 and LDL-2 were positively associated with <i>Lachnospiraceae</i> and <i>Coriobacteriaceae</i>, and negatively with <i>Bacteroidaceae</i> and <i>Bifidobacteriaceae</i>. Metagenome-sequencing showed a higher abundance of biosynthesis of multiple B-vitamins and SCFA metabolism genes among healthier LPD profiles. Metagenomic-assembled genomes (MAGs) affiliated to <i>Eggerthellaceae</i> and <i>Clostridiales</i> were contributors of these genes and their relative abundance correlated positively with larger HDL subfractions. The study demonstrates that differences in composition and metabolic traits of the GM are associated with variations in LPD among the recruited subjects. These findings provide evidence for GM considerations in future research aiming to shed light on mechanisms of the GM–dyslipidemia axis. |
| first_indexed | 2024-03-09T18:49:32Z |
| format | Article |
| id | doaj.art-026a3f7a04784f22a5e6e072def7a2f9 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T18:49:32Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-026a3f7a04784f22a5e6e072def7a2f92023-11-24T05:56:41ZengMDPI AGMicroorganisms2076-26072022-10-011011215610.3390/microorganisms10112156Gut Microbiome and Its Cofactors Are Linked to Lipoprotein Distribution ProfilesJosué L. Castro-Mejía0Bekzod Khakimov1Violetta Aru2Mads V. Lind3Eva Garne4Petronela Paulová5Elnaz Tavakkoli6Lars H. Hansen7Age K. Smilde8Lars Holm9Søren B. Engelsen10Dennis S. Nielsen11Department of Food Science, University of Copenhagen, 1958 Frederiksberg C, DenmarkDepartment of Food Science, University of Copenhagen, 1958 Frederiksberg C, DenmarkDepartment of Food Science, University of Copenhagen, 1958 Frederiksberg C, DenmarkDepartment of Nutrition, Exercise and Sports, University of Copenhagen, 1958 Frederiksberg C, DenmarkDepartment of Food Science, University of Copenhagen, 1958 Frederiksberg C, DenmarkDepartment of Food Science, University of Copenhagen, 1958 Frederiksberg C, DenmarkSwammerdam Institute for Life Sciences, University of Amsterdam, Postbus 94215, 1090 GE Amsterdam, The NetherlandsDepartment of Plant and Environmental Sciences, University of Copenhagen, 1871 Frederiksberg C, DenmarkDepartment of Food Science, University of Copenhagen, 1958 Frederiksberg C, DenmarkDepartment of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen N, DenmarkDepartment of Food Science, University of Copenhagen, 1958 Frederiksberg C, DenmarkDepartment of Food Science, University of Copenhagen, 1958 Frederiksberg C, DenmarkIncreasing evidence indicates that the gut microbiome (GM) plays an important role in dyslipidemia. To date, however, no in-depth characterization of the associations between GM with lipoproteins distributions (LPD) among adult individuals with diverse BMI has been conducted. To determine such associations, we studied blood-plasma LPD, fecal short-chain fatty acids (SCFA) and GM of 262 Danes aged 19–89 years. Stratification of LPD segregated subjects into three clusters displaying recommended levels of lipoproteins and explained by age and body-mass-index. Higher levels of HDL2a and HDL2b were associated with a higher abundance of <i>Ruminococcaceae</i> and <i>Christensenellaceae</i>. Increasing levels of total cholesterol and LDL-1 and LDL-2 were positively associated with <i>Lachnospiraceae</i> and <i>Coriobacteriaceae</i>, and negatively with <i>Bacteroidaceae</i> and <i>Bifidobacteriaceae</i>. Metagenome-sequencing showed a higher abundance of biosynthesis of multiple B-vitamins and SCFA metabolism genes among healthier LPD profiles. Metagenomic-assembled genomes (MAGs) affiliated to <i>Eggerthellaceae</i> and <i>Clostridiales</i> were contributors of these genes and their relative abundance correlated positively with larger HDL subfractions. The study demonstrates that differences in composition and metabolic traits of the GM are associated with variations in LPD among the recruited subjects. These findings provide evidence for GM considerations in future research aiming to shed light on mechanisms of the GM–dyslipidemia axis.https://www.mdpi.com/2076-2607/10/11/2156gut microbiotaSCFAslipoproteins distributionHDL<sup>1</sup>H-NMR |
| spellingShingle | Josué L. Castro-Mejía Bekzod Khakimov Violetta Aru Mads V. Lind Eva Garne Petronela Paulová Elnaz Tavakkoli Lars H. Hansen Age K. Smilde Lars Holm Søren B. Engelsen Dennis S. Nielsen Gut Microbiome and Its Cofactors Are Linked to Lipoprotein Distribution Profiles Microorganisms gut microbiota SCFAs lipoproteins distribution HDL <sup>1</sup>H-NMR |
| title | Gut Microbiome and Its Cofactors Are Linked to Lipoprotein Distribution Profiles |
| title_full | Gut Microbiome and Its Cofactors Are Linked to Lipoprotein Distribution Profiles |
| title_fullStr | Gut Microbiome and Its Cofactors Are Linked to Lipoprotein Distribution Profiles |
| title_full_unstemmed | Gut Microbiome and Its Cofactors Are Linked to Lipoprotein Distribution Profiles |
| title_short | Gut Microbiome and Its Cofactors Are Linked to Lipoprotein Distribution Profiles |
| title_sort | gut microbiome and its cofactors are linked to lipoprotein distribution profiles |
| topic | gut microbiota SCFAs lipoproteins distribution HDL <sup>1</sup>H-NMR |
| url | https://www.mdpi.com/2076-2607/10/11/2156 |
| work_keys_str_mv | AT josuelcastromejia gutmicrobiomeanditscofactorsarelinkedtolipoproteindistributionprofiles AT bekzodkhakimov gutmicrobiomeanditscofactorsarelinkedtolipoproteindistributionprofiles AT violettaaru gutmicrobiomeanditscofactorsarelinkedtolipoproteindistributionprofiles AT madsvlind gutmicrobiomeanditscofactorsarelinkedtolipoproteindistributionprofiles AT evagarne gutmicrobiomeanditscofactorsarelinkedtolipoproteindistributionprofiles AT petronelapaulova gutmicrobiomeanditscofactorsarelinkedtolipoproteindistributionprofiles AT elnaztavakkoli gutmicrobiomeanditscofactorsarelinkedtolipoproteindistributionprofiles AT larshhansen gutmicrobiomeanditscofactorsarelinkedtolipoproteindistributionprofiles AT ageksmilde gutmicrobiomeanditscofactorsarelinkedtolipoproteindistributionprofiles AT larsholm gutmicrobiomeanditscofactorsarelinkedtolipoproteindistributionprofiles AT sørenbengelsen gutmicrobiomeanditscofactorsarelinkedtolipoproteindistributionprofiles AT dennissnielsen gutmicrobiomeanditscofactorsarelinkedtolipoproteindistributionprofiles |