Using recombinant Lactococci as an approach to dissect the immunomodulating capacity of surface piliation in probiotic Lactobacillus rhamnosus GG.
Primarily arising from their well understood beneficial health effects, many lactobacilli strains are considered good candidates for use as probiotics in humans and animals. Lactobacillar probiosis can itself be best typified by the Lactobacillus rhamnosus GG strain, which, with its well-documented...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3653913?pdf=render |
| _version_ | 1818928349043490816 |
|---|---|
| author | Ingemar von Ossowski Taija E Pietilä Johanna Rintahaka Elina Nummenmaa Veli-Matti Mäkinen Justus Reunanen Reetta Satokari Willem M de Vos Ilkka Palva Airi Palva |
| author_facet | Ingemar von Ossowski Taija E Pietilä Johanna Rintahaka Elina Nummenmaa Veli-Matti Mäkinen Justus Reunanen Reetta Satokari Willem M de Vos Ilkka Palva Airi Palva |
| author_sort | Ingemar von Ossowski |
| collection | DOAJ |
| description | Primarily arising from their well understood beneficial health effects, many lactobacilli strains are considered good candidates for use as probiotics in humans and animals. Lactobacillar probiosis can itself be best typified by the Lactobacillus rhamnosus GG strain, which, with its well-documented clinical benefits, has emerged as one of the most widely used probiotics in the food and health-supplement industries. Even so, many facets of its molecular mechanisms and limitations as a beneficial commensal bacterium still remain to be thoroughly explored and dissected. Because L. rhamnosus GG is one of only a few such strains exhibiting surface piliation (called SpaCBA), we sought to examine whether this particular type of cell-surface appendage has a discernible immunomodulating capacity and is able to trigger targeted responses in human immune-related cells. Thus, presented herein for this study, we recombinantly engineered Lactococcus lactis to produce native (and pilin-deleted) SpaCBA pili that were assembled in a structurally authentic form and anchored to the cell surface, and which had retained mucus-binding functionality. By using these recombinant lactococcal constructs, we were able to demonstrate that the SpaCBA pilus can be a contributory factor in the activation of Toll-like receptor 2-dependent signaling in HEK cells as well as in the modulation of pro- and anti-inflammatory cytokine (TNF-α, IL-6, IL-10, and IL-12) production in human monocyte-derived dendritic cells. From these data, we suggest that the recombinant-expressed and surface-anchored SpaCBA pilus, given its projected functioning in the gut environment, might be viewed as a new microbe-associated molecular pattern (MAMP)-like modulator of innate immunity. Accordingly, our study has brought some new insight to the molecular immunogenicity of the SpaCBA pilus, thus opening the way to a better understanding of its possible role in the multifaceted nature of L. rhamnosus GG probiosis within the human gut. |
| first_indexed | 2024-12-20T03:27:29Z |
| format | Article |
| id | doaj.art-026e77a49b594ac3bc9a06f65f46d3f9 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T03:27:29Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-026e77a49b594ac3bc9a06f65f46d3f92022-12-21T19:55:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0185e6441610.1371/journal.pone.0064416Using recombinant Lactococci as an approach to dissect the immunomodulating capacity of surface piliation in probiotic Lactobacillus rhamnosus GG.Ingemar von OssowskiTaija E PietiläJohanna RintahakaElina NummenmaaVeli-Matti MäkinenJustus ReunanenReetta SatokariWillem M de VosIlkka PalvaAiri PalvaPrimarily arising from their well understood beneficial health effects, many lactobacilli strains are considered good candidates for use as probiotics in humans and animals. Lactobacillar probiosis can itself be best typified by the Lactobacillus rhamnosus GG strain, which, with its well-documented clinical benefits, has emerged as one of the most widely used probiotics in the food and health-supplement industries. Even so, many facets of its molecular mechanisms and limitations as a beneficial commensal bacterium still remain to be thoroughly explored and dissected. Because L. rhamnosus GG is one of only a few such strains exhibiting surface piliation (called SpaCBA), we sought to examine whether this particular type of cell-surface appendage has a discernible immunomodulating capacity and is able to trigger targeted responses in human immune-related cells. Thus, presented herein for this study, we recombinantly engineered Lactococcus lactis to produce native (and pilin-deleted) SpaCBA pili that were assembled in a structurally authentic form and anchored to the cell surface, and which had retained mucus-binding functionality. By using these recombinant lactococcal constructs, we were able to demonstrate that the SpaCBA pilus can be a contributory factor in the activation of Toll-like receptor 2-dependent signaling in HEK cells as well as in the modulation of pro- and anti-inflammatory cytokine (TNF-α, IL-6, IL-10, and IL-12) production in human monocyte-derived dendritic cells. From these data, we suggest that the recombinant-expressed and surface-anchored SpaCBA pilus, given its projected functioning in the gut environment, might be viewed as a new microbe-associated molecular pattern (MAMP)-like modulator of innate immunity. Accordingly, our study has brought some new insight to the molecular immunogenicity of the SpaCBA pilus, thus opening the way to a better understanding of its possible role in the multifaceted nature of L. rhamnosus GG probiosis within the human gut.http://europepmc.org/articles/PMC3653913?pdf=render |
| spellingShingle | Ingemar von Ossowski Taija E Pietilä Johanna Rintahaka Elina Nummenmaa Veli-Matti Mäkinen Justus Reunanen Reetta Satokari Willem M de Vos Ilkka Palva Airi Palva Using recombinant Lactococci as an approach to dissect the immunomodulating capacity of surface piliation in probiotic Lactobacillus rhamnosus GG. PLoS ONE |
| title | Using recombinant Lactococci as an approach to dissect the immunomodulating capacity of surface piliation in probiotic Lactobacillus rhamnosus GG. |
| title_full | Using recombinant Lactococci as an approach to dissect the immunomodulating capacity of surface piliation in probiotic Lactobacillus rhamnosus GG. |
| title_fullStr | Using recombinant Lactococci as an approach to dissect the immunomodulating capacity of surface piliation in probiotic Lactobacillus rhamnosus GG. |
| title_full_unstemmed | Using recombinant Lactococci as an approach to dissect the immunomodulating capacity of surface piliation in probiotic Lactobacillus rhamnosus GG. |
| title_short | Using recombinant Lactococci as an approach to dissect the immunomodulating capacity of surface piliation in probiotic Lactobacillus rhamnosus GG. |
| title_sort | using recombinant lactococci as an approach to dissect the immunomodulating capacity of surface piliation in probiotic lactobacillus rhamnosus gg |
| url | http://europepmc.org/articles/PMC3653913?pdf=render |
| work_keys_str_mv | AT ingemarvonossowski usingrecombinantlactococciasanapproachtodissecttheimmunomodulatingcapacityofsurfacepiliationinprobioticlactobacillusrhamnosusgg AT taijaepietila usingrecombinantlactococciasanapproachtodissecttheimmunomodulatingcapacityofsurfacepiliationinprobioticlactobacillusrhamnosusgg AT johannarintahaka usingrecombinantlactococciasanapproachtodissecttheimmunomodulatingcapacityofsurfacepiliationinprobioticlactobacillusrhamnosusgg AT elinanummenmaa usingrecombinantlactococciasanapproachtodissecttheimmunomodulatingcapacityofsurfacepiliationinprobioticlactobacillusrhamnosusgg AT velimattimakinen usingrecombinantlactococciasanapproachtodissecttheimmunomodulatingcapacityofsurfacepiliationinprobioticlactobacillusrhamnosusgg AT justusreunanen usingrecombinantlactococciasanapproachtodissecttheimmunomodulatingcapacityofsurfacepiliationinprobioticlactobacillusrhamnosusgg AT reettasatokari usingrecombinantlactococciasanapproachtodissecttheimmunomodulatingcapacityofsurfacepiliationinprobioticlactobacillusrhamnosusgg AT willemmdevos usingrecombinantlactococciasanapproachtodissecttheimmunomodulatingcapacityofsurfacepiliationinprobioticlactobacillusrhamnosusgg AT ilkkapalva usingrecombinantlactococciasanapproachtodissecttheimmunomodulatingcapacityofsurfacepiliationinprobioticlactobacillusrhamnosusgg AT airipalva usingrecombinantlactococciasanapproachtodissecttheimmunomodulatingcapacityofsurfacepiliationinprobioticlactobacillusrhamnosusgg |