In vitro evaluation of the effect of galectins on Schistosoma mansoni motility
Abstract Objective Galectins are sugar-binding proteins that participate in many biological processes, such as immunity, by regulating host immune cells and their direct interaction with pathogens. They are involved in mediating infection by Schistosoma mansoni, a parasitic trematode that causes sch...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-023-06530-9 |
| _version_ | 1797453991166083072 |
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| author | Tomoharu Takeuchi Risa Nakamura Megumi Hamasaki Midori Oyama Shinjiro Hamano Tomomi Hatanaka |
| author_facet | Tomoharu Takeuchi Risa Nakamura Megumi Hamasaki Midori Oyama Shinjiro Hamano Tomomi Hatanaka |
| author_sort | Tomoharu Takeuchi |
| collection | DOAJ |
| description | Abstract Objective Galectins are sugar-binding proteins that participate in many biological processes, such as immunity, by regulating host immune cells and their direct interaction with pathogens. They are involved in mediating infection by Schistosoma mansoni, a parasitic trematode that causes schistosomiasis. However, their direct effects on schistosomes have not been investigated. Results We found that galectin-2 recognizes S. mansoni glycoconjugates and investigated whether galectin-1, 2, and 3 can directly affect S. mansoni in vitro. Adult S. mansoni were treated with recombinant galectin-1, 2, and 3 proteins or praziquantel, a positive control. Treatment with galectin-1, 2, and 3 had no significant effect on S. mansoni motility, and no other differences were observed under a stereoscopic microscope. Hence, galectin-1, 2, and 3 may have a little direct effect on S. mansoni. However, they might play a role in the infection in vivo via the modulation of the host immune response or secretory molecules from S. mansoni. To the best of our knowledge, this is the first study to investigate the direct effect of galectins on S. mansoni and helps in understanding the roles of galectins in S. mansoni infection in vivo. |
| first_indexed | 2024-03-09T15:30:47Z |
| format | Article |
| id | doaj.art-026fa76716be47e183aa3601d911ee45 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-03-09T15:30:47Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-026fa76716be47e183aa3601d911ee452023-11-26T12:14:34ZengBMCBMC Research Notes1756-05002023-10-011611710.1186/s13104-023-06530-9In vitro evaluation of the effect of galectins on Schistosoma mansoni motilityTomoharu Takeuchi0Risa Nakamura1Megumi Hamasaki2Midori Oyama3Shinjiro Hamano4Tomomi Hatanaka5Laboratory of Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Josai UniversityDepartment of Parasitology, Nagasaki University, Institute of Tropical Medicine (NEKKEN)Department of Parasitology, Nagasaki University, Institute of Tropical Medicine (NEKKEN)Laboratory of Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Josai UniversityDepartment of Parasitology, Nagasaki University, Institute of Tropical Medicine (NEKKEN)Laboratory of Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Josai UniversityAbstract Objective Galectins are sugar-binding proteins that participate in many biological processes, such as immunity, by regulating host immune cells and their direct interaction with pathogens. They are involved in mediating infection by Schistosoma mansoni, a parasitic trematode that causes schistosomiasis. However, their direct effects on schistosomes have not been investigated. Results We found that galectin-2 recognizes S. mansoni glycoconjugates and investigated whether galectin-1, 2, and 3 can directly affect S. mansoni in vitro. Adult S. mansoni were treated with recombinant galectin-1, 2, and 3 proteins or praziquantel, a positive control. Treatment with galectin-1, 2, and 3 had no significant effect on S. mansoni motility, and no other differences were observed under a stereoscopic microscope. Hence, galectin-1, 2, and 3 may have a little direct effect on S. mansoni. However, they might play a role in the infection in vivo via the modulation of the host immune response or secretory molecules from S. mansoni. To the best of our knowledge, this is the first study to investigate the direct effect of galectins on S. mansoni and helps in understanding the roles of galectins in S. mansoni infection in vivo.https://doi.org/10.1186/s13104-023-06530-9GalectinGlycanLectinParasiteSchistosoma mansoni |
| spellingShingle | Tomoharu Takeuchi Risa Nakamura Megumi Hamasaki Midori Oyama Shinjiro Hamano Tomomi Hatanaka In vitro evaluation of the effect of galectins on Schistosoma mansoni motility BMC Research Notes Galectin Glycan Lectin Parasite Schistosoma mansoni |
| title | In vitro evaluation of the effect of galectins on Schistosoma mansoni motility |
| title_full | In vitro evaluation of the effect of galectins on Schistosoma mansoni motility |
| title_fullStr | In vitro evaluation of the effect of galectins on Schistosoma mansoni motility |
| title_full_unstemmed | In vitro evaluation of the effect of galectins on Schistosoma mansoni motility |
| title_short | In vitro evaluation of the effect of galectins on Schistosoma mansoni motility |
| title_sort | in vitro evaluation of the effect of galectins on schistosoma mansoni motility |
| topic | Galectin Glycan Lectin Parasite Schistosoma mansoni |
| url | https://doi.org/10.1186/s13104-023-06530-9 |
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