| Summary: | Extraordinary properties of two-dimensional materials make them attractive for applications in different fields. One of the prospective niches is optical applications, where such types of materials demonstrate extremely sensitive performance and can be used for labeling. However, the optical properties of liquid-exfoliated 2D materials need to be analyzed. The purpose of this work is to study the absorption and luminescent properties of MoS<sub>2</sub> exfoliated in the presence of sodium cholate, which is the most often used surfactant. Ultrasound bath and mixer-assisted exfoliation in water and dimethyl sulfoxide were used. The best quality of MoS<sub>2</sub> nanosheets was achieved using shear-assisted liquid-phase exfoliation as a production method and sodium cholate (SC) as a surfactant. The photoluminescent properties of MoS<sub>2</sub> nanosheets varied slightly when changing the surfactant concentrations in the range C(SC) = 0.5–2.5 mg/mL. This work is of high practical importance for further enhancement of MoS<sub>2</sub> photoluminescent properties via chemical functionalization.
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