Gut Microbiota and Short-Chain Fatty Acid Profile between Normal and Moderate Malnutrition Children in Yogyakarta, Indonesia
Malnutrition has been associated with the gut microbiota composition and the gastrointestinal environment. This study aimed to evaluate whether there is a difference in the gut microbiota profile between the normal and undernutrition (considered moderate malnutrition) children and evaluate the gastr...
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MDPI AG
2021-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/1/127 |
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| author | Rafli Zulfa Kamil Agnes Murdiati Mohammad Juffrie Jiro Nakayama Endang Sutriswati Rahayu |
| author_facet | Rafli Zulfa Kamil Agnes Murdiati Mohammad Juffrie Jiro Nakayama Endang Sutriswati Rahayu |
| author_sort | Rafli Zulfa Kamil |
| collection | DOAJ |
| description | Malnutrition has been associated with the gut microbiota composition and the gastrointestinal environment. This study aimed to evaluate whether there is a difference in the gut microbiota profile between the normal and undernutrition (considered moderate malnutrition) children and evaluate the gastrointestinal environment observed from the short-chain fatty acid (SCFA) profile. Ten days’ observations were done between normal (<i>n</i>:13) and undernutrition (<i>n</i>:15) children. The subject’s diet was recorded using a food record. Analysis of the gut microbiota was performed using 16S rRNA gene sequencing targeting the V3-V4 variables region, while the SCFA profile was analyzed using gas chromatography. The result shows that the undernutrition group’s energy intake was lower than in the normal group. Although there was no difference in diversity index and overall gut composition, overexpression of the genera <i>Methanobrevibacter</i>, <i>Anaerococcus</i>, <i>Eubacterium</i>, and <i>Succinivibrio</i> was observed in the undernutrition group. Meanwhile, in the normal group, <i>Ruminococcus</i> and <i>Fusobacterium</i> were found. In both groups, there was also the dominant of <i>Prevotella</i> enterotype. Gastrointestinal conditions in the normal group tended to be more acidic compared to the undernutrition group. It occurs due to the high concentration of propionate and butyric acids. |
| first_indexed | 2024-03-09T05:41:47Z |
| format | Article |
| id | doaj.art-0271e1a8c38f4ae6ba44f6c727e1ffc8 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T05:41:47Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-0271e1a8c38f4ae6ba44f6c727e1ffc82023-12-03T12:24:22ZengMDPI AGMicroorganisms2076-26072021-01-019112710.3390/microorganisms9010127Gut Microbiota and Short-Chain Fatty Acid Profile between Normal and Moderate Malnutrition Children in Yogyakarta, IndonesiaRafli Zulfa Kamil0Agnes Murdiati1Mohammad Juffrie2Jiro Nakayama3Endang Sutriswati Rahayu4Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No 1 Bulaksumur, Yogyakarta 55281, IndonesiaDepartment of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No 1 Bulaksumur, Yogyakarta 55281, IndonesiaFaculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Jl. Farmako, Senolowo, Sekip Utara, Yogyakarta 55281, IndonesiaDepartment of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, JapanDepartment of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No 1 Bulaksumur, Yogyakarta 55281, IndonesiaMalnutrition has been associated with the gut microbiota composition and the gastrointestinal environment. This study aimed to evaluate whether there is a difference in the gut microbiota profile between the normal and undernutrition (considered moderate malnutrition) children and evaluate the gastrointestinal environment observed from the short-chain fatty acid (SCFA) profile. Ten days’ observations were done between normal (<i>n</i>:13) and undernutrition (<i>n</i>:15) children. The subject’s diet was recorded using a food record. Analysis of the gut microbiota was performed using 16S rRNA gene sequencing targeting the V3-V4 variables region, while the SCFA profile was analyzed using gas chromatography. The result shows that the undernutrition group’s energy intake was lower than in the normal group. Although there was no difference in diversity index and overall gut composition, overexpression of the genera <i>Methanobrevibacter</i>, <i>Anaerococcus</i>, <i>Eubacterium</i>, and <i>Succinivibrio</i> was observed in the undernutrition group. Meanwhile, in the normal group, <i>Ruminococcus</i> and <i>Fusobacterium</i> were found. In both groups, there was also the dominant of <i>Prevotella</i> enterotype. Gastrointestinal conditions in the normal group tended to be more acidic compared to the undernutrition group. It occurs due to the high concentration of propionate and butyric acids.https://www.mdpi.com/2076-2607/9/1/127gut microbiotaSCFAmoderate malnutrition |
| spellingShingle | Rafli Zulfa Kamil Agnes Murdiati Mohammad Juffrie Jiro Nakayama Endang Sutriswati Rahayu Gut Microbiota and Short-Chain Fatty Acid Profile between Normal and Moderate Malnutrition Children in Yogyakarta, Indonesia Microorganisms gut microbiota SCFA moderate malnutrition |
| title | Gut Microbiota and Short-Chain Fatty Acid Profile between Normal and Moderate Malnutrition Children in Yogyakarta, Indonesia |
| title_full | Gut Microbiota and Short-Chain Fatty Acid Profile between Normal and Moderate Malnutrition Children in Yogyakarta, Indonesia |
| title_fullStr | Gut Microbiota and Short-Chain Fatty Acid Profile between Normal and Moderate Malnutrition Children in Yogyakarta, Indonesia |
| title_full_unstemmed | Gut Microbiota and Short-Chain Fatty Acid Profile between Normal and Moderate Malnutrition Children in Yogyakarta, Indonesia |
| title_short | Gut Microbiota and Short-Chain Fatty Acid Profile between Normal and Moderate Malnutrition Children in Yogyakarta, Indonesia |
| title_sort | gut microbiota and short chain fatty acid profile between normal and moderate malnutrition children in yogyakarta indonesia |
| topic | gut microbiota SCFA moderate malnutrition |
| url | https://www.mdpi.com/2076-2607/9/1/127 |
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