Change in energy metabolism of in vitro produced embryos: an alternative to make them more cryoresistant?
For the development of in vitro produced (IVP) as well as in vivo produced bovine embryos, it is extremely important that their energy metabolism works properly because the embryo must be able to metabolize energy substrates that are necessary for producing energy. Lipids play an important role in e...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2017-08-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/26534 |
| _version_ | 1818526014008983552 |
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| author | Luzia Renata Oliveira Dias Ivo Pivato Margot Alves Nunes Dode |
| author_facet | Luzia Renata Oliveira Dias Ivo Pivato Margot Alves Nunes Dode |
| author_sort | Luzia Renata Oliveira Dias |
| collection | DOAJ |
| description | For the development of in vitro produced (IVP) as well as in vivo produced bovine embryos, it is extremely important that their energy metabolism works properly because the embryo must be able to metabolize energy substrates that are necessary for producing energy. Lipids play an important role in early embryonic development, acting as source of energy for oocytes and embryos. However, it is known that oocytes and embryos, mainly IVP, accumulate large amounts of lipids in the cytoplasm. Although they are extremely important in embryonic development, lipids have been associated with the reduced survival of bovine embryos following cryopreservation. There is evidence that at least four different categories of lipids affect embryo survival after cryopreservation, including triglycerides (TAG), free fatty acids, cholesterol and phospholipids. Thus, many studies are being conducted to improve the resistance of IVP embryos to the cryopreservation process by reducing the concentration or removing the source of serum from the medium or by reducing oocyte/embryo lipids using mechanical or chemical means. Regarding the use of delipidating agents that reduce the uptake and synthesis of fatty acids (FA) by cells, substances such as phenazine ethosulfate (PES), forskolin, L-carnitine and isomers of conjugated linoleic acid (CLA) have been utilized. This review aims to address important issues related to embryonic energy metabolism, the importance of lipid metabolism and its relation to the cryopreservation of IVP bovine embryos by summarizing the latest research in this field. |
| first_indexed | 2024-12-11T06:17:14Z |
| format | Article |
| id | doaj.art-02721f50557646a4af9e9f96335885fd |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-12-11T06:17:14Z |
| publishDate | 2017-08-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-02721f50557646a4af9e9f96335885fd2022-12-22T01:17:57ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592017-08-013842237225410.5433/1679-0359.2017v38n4p223715566Change in energy metabolism of in vitro produced embryos: an alternative to make them more cryoresistant?Luzia Renata Oliveira Dias0Ivo Pivato1Margot Alves Nunes Dode2Universidade de BrasíliaUniversidade de BrasíliaEmbrapa Recursos Genéticos e BiotecnologiaFor the development of in vitro produced (IVP) as well as in vivo produced bovine embryos, it is extremely important that their energy metabolism works properly because the embryo must be able to metabolize energy substrates that are necessary for producing energy. Lipids play an important role in early embryonic development, acting as source of energy for oocytes and embryos. However, it is known that oocytes and embryos, mainly IVP, accumulate large amounts of lipids in the cytoplasm. Although they are extremely important in embryonic development, lipids have been associated with the reduced survival of bovine embryos following cryopreservation. There is evidence that at least four different categories of lipids affect embryo survival after cryopreservation, including triglycerides (TAG), free fatty acids, cholesterol and phospholipids. Thus, many studies are being conducted to improve the resistance of IVP embryos to the cryopreservation process by reducing the concentration or removing the source of serum from the medium or by reducing oocyte/embryo lipids using mechanical or chemical means. Regarding the use of delipidating agents that reduce the uptake and synthesis of fatty acids (FA) by cells, substances such as phenazine ethosulfate (PES), forskolin, L-carnitine and isomers of conjugated linoleic acid (CLA) have been utilized. This review aims to address important issues related to embryonic energy metabolism, the importance of lipid metabolism and its relation to the cryopreservation of IVP bovine embryos by summarizing the latest research in this field.http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/26534CriopreservaçãoEmbriões bovinosLipídiosMetabolismo energético. |
| spellingShingle | Luzia Renata Oliveira Dias Ivo Pivato Margot Alves Nunes Dode Change in energy metabolism of in vitro produced embryos: an alternative to make them more cryoresistant? Semina: Ciências Agrárias Criopreservação Embriões bovinos Lipídios Metabolismo energético. |
| title | Change in energy metabolism of in vitro produced embryos: an alternative to make them more cryoresistant? |
| title_full | Change in energy metabolism of in vitro produced embryos: an alternative to make them more cryoresistant? |
| title_fullStr | Change in energy metabolism of in vitro produced embryos: an alternative to make them more cryoresistant? |
| title_full_unstemmed | Change in energy metabolism of in vitro produced embryos: an alternative to make them more cryoresistant? |
| title_short | Change in energy metabolism of in vitro produced embryos: an alternative to make them more cryoresistant? |
| title_sort | change in energy metabolism of in vitro produced embryos an alternative to make them more cryoresistant |
| topic | Criopreservação Embriões bovinos Lipídios Metabolismo energético. |
| url | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/26534 |
| work_keys_str_mv | AT luziarenataoliveiradias changeinenergymetabolismofinvitroproducedembryosanalternativetomakethemmorecryoresistant AT ivopivato changeinenergymetabolismofinvitroproducedembryosanalternativetomakethemmorecryoresistant AT margotalvesnunesdode changeinenergymetabolismofinvitroproducedembryosanalternativetomakethemmorecryoresistant |