Monitoring Resistance of <i>Euschistus heros</i> (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay
The novel ingestion bioassay method was developed for detecting and monitoring resistance of <i>Euschistus heros</i> by encapsulating an artificial liquid diet using Parafilm<sup>®</sup>. This methodology was compared with the tarsal contact (vial test) and topical applicatio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/7/599 |
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| author | Cristiane Maria Tibola Leandro Silva Fernanda Sgubin Celso Omoto |
| author_facet | Cristiane Maria Tibola Leandro Silva Fernanda Sgubin Celso Omoto |
| author_sort | Cristiane Maria Tibola |
| collection | DOAJ |
| description | The novel ingestion bioassay method was developed for detecting and monitoring resistance of <i>Euschistus heros</i> by encapsulating an artificial liquid diet using Parafilm<sup>®</sup>. This methodology was compared with the tarsal contact (vial test) and topical application methods for thiamethoxam, imidacloprid, and lambda-cyhalothrin. The best bioassay method for the neonicotinoid insecticides thiamethoxam and imidacloprid was ingestion. For pyrethroid insecticide lambda-cyhalothrin, the best result was obtained by topical application. Using the best bioassay method for each insecticide, the susceptibility to these insecticides was monitored in 30 populations of <i>E. heros</i> collected from soybean crops in Brazil from 2018 to 2020. High variations in susceptibility to thiamethoxam (resistance ratios, 1.6–22 times), imidacloprid (resistance ratios, 1.6–22 times), and lambda-cyhalothrin (resistance ratios, 5–40 times) were detected among the evaluated <i>E. heros</i> populations. In order to monitor the susceptibility of <i>E. heros</i> to insecticides, diagnostic concentrations were defined based on the LC<sub>99</sub> of the susceptible reference population: 5.65 µL of a.i./mL for thiamethoxam, 12.45 µL of a.i./mL for imidacloprid, and 0.20 µg of a.i./insect for lambda-cyhalothrin. Subsequently, we select an <i>E. heros</i> strain resistant to neonicotinoid insecticides and another to lambda-cyhalothrin. The resistance ratios obtained after seven selection cycles were 66, 41 and 44 times for thiamethoxam, imidacloprid and lambda-cyhalothrin, respectively. |
| first_indexed | 2024-03-10T09:35:54Z |
| format | Article |
| id | doaj.art-02741e3f7b084c23b95d699d9aa9f4e0 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T09:35:54Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-02741e3f7b084c23b95d699d9aa9f4e02023-11-22T04:04:12ZengMDPI AGInsects2075-44502021-07-0112759910.3390/insects12070599Monitoring Resistance of <i>Euschistus heros</i> (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet BioassayCristiane Maria Tibola0Leandro Silva1Fernanda Sgubin2Celso Omoto3Department of Entomology and Acarology, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba 13418-900, SP, BrazilCL Empreendimentos Biológicos Ltd., Piracicaba 13424-586, SP, BrazilCL Empreendimentos Biológicos Ltd., Piracicaba 13424-586, SP, BrazilDepartment of Entomology and Acarology, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba 13418-900, SP, BrazilThe novel ingestion bioassay method was developed for detecting and monitoring resistance of <i>Euschistus heros</i> by encapsulating an artificial liquid diet using Parafilm<sup>®</sup>. This methodology was compared with the tarsal contact (vial test) and topical application methods for thiamethoxam, imidacloprid, and lambda-cyhalothrin. The best bioassay method for the neonicotinoid insecticides thiamethoxam and imidacloprid was ingestion. For pyrethroid insecticide lambda-cyhalothrin, the best result was obtained by topical application. Using the best bioassay method for each insecticide, the susceptibility to these insecticides was monitored in 30 populations of <i>E. heros</i> collected from soybean crops in Brazil from 2018 to 2020. High variations in susceptibility to thiamethoxam (resistance ratios, 1.6–22 times), imidacloprid (resistance ratios, 1.6–22 times), and lambda-cyhalothrin (resistance ratios, 5–40 times) were detected among the evaluated <i>E. heros</i> populations. In order to monitor the susceptibility of <i>E. heros</i> to insecticides, diagnostic concentrations were defined based on the LC<sub>99</sub> of the susceptible reference population: 5.65 µL of a.i./mL for thiamethoxam, 12.45 µL of a.i./mL for imidacloprid, and 0.20 µg of a.i./insect for lambda-cyhalothrin. Subsequently, we select an <i>E. heros</i> strain resistant to neonicotinoid insecticides and another to lambda-cyhalothrin. The resistance ratios obtained after seven selection cycles were 66, 41 and 44 times for thiamethoxam, imidacloprid and lambda-cyhalothrin, respectively.https://www.mdpi.com/2075-4450/12/7/599<i>Euschistus heros</i>bioassayNeotropical brown stink buginsecticide resistance managementIRM |
| spellingShingle | Cristiane Maria Tibola Leandro Silva Fernanda Sgubin Celso Omoto Monitoring Resistance of <i>Euschistus heros</i> (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay Insects <i>Euschistus heros</i> bioassay Neotropical brown stink bug insecticide resistance management IRM |
| title | Monitoring Resistance of <i>Euschistus heros</i> (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay |
| title_full | Monitoring Resistance of <i>Euschistus heros</i> (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay |
| title_fullStr | Monitoring Resistance of <i>Euschistus heros</i> (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay |
| title_full_unstemmed | Monitoring Resistance of <i>Euschistus heros</i> (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay |
| title_short | Monitoring Resistance of <i>Euschistus heros</i> (Fabricius) (Hemiptera: Pentatomidae) to Insecticides by Using Encapsulated Artificial Diet Bioassay |
| title_sort | monitoring resistance of i euschistus heros i fabricius hemiptera pentatomidae to insecticides by using encapsulated artificial diet bioassay |
| topic | <i>Euschistus heros</i> bioassay Neotropical brown stink bug insecticide resistance management IRM |
| url | https://www.mdpi.com/2075-4450/12/7/599 |
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