Carriage of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Staphylococcus aureus in Indonesian children: A cross-sectional study.
Streptococcus pneumoniae is an important cause of infection and commonly colonizes the nasopharynx of young children, along with other potentially pathogenic bacteria. The objectives of this study were to estimate the carriage prevalence of S. pneumoniae, Haemophilus influenzae, Moraxella catarrhali...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5896896?pdf=render |
| _version_ | 1818258656094846976 |
|---|---|
| author | Eileen M Dunne Chrysanti Murad Sunaryati Sudigdoadi Eddy Fadlyana Rodman Tarigan Sang Ayu Kompiyang Indriyani Casey L Pell Emma Watts Catherine Satzke Jason Hinds Nurhandini Eka Dewi Finny Fitry Yani Kusnandi Rusmil E Kim Mulholland Cissy Kartasasmita |
| author_facet | Eileen M Dunne Chrysanti Murad Sunaryati Sudigdoadi Eddy Fadlyana Rodman Tarigan Sang Ayu Kompiyang Indriyani Casey L Pell Emma Watts Catherine Satzke Jason Hinds Nurhandini Eka Dewi Finny Fitry Yani Kusnandi Rusmil E Kim Mulholland Cissy Kartasasmita |
| author_sort | Eileen M Dunne |
| collection | DOAJ |
| description | Streptococcus pneumoniae is an important cause of infection and commonly colonizes the nasopharynx of young children, along with other potentially pathogenic bacteria. The objectives of this study were to estimate the carriage prevalence of S. pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Staphylococcus aureus in young children in Indonesia, and to examine interactions between these bacterial species. 302 healthy children aged 12-24 months were enrolled in community health centers in the Bandung, Central Lombok, and Padang regions. Nasopharyngeal swabs were collected and stored according to World Health Organization recommendations, and bacterial species detected by qPCR. Pneumococcal serotyping was conducted by microarray and latex agglutination/Quellung. Overall carriage prevalence was 49.5% for S. pneumoniae, 27.5% for H. influenzae, 42.7% for M. catarrhalis, and 7.3% for S. aureus. Prevalence of M. catarrhalis and S. pneumoniae, as well as pneumococcal serotype distribution, varied by region. Positive associations were observed for S. pneumoniae and M. catarrhalis (OR 3.07 [95%CI 1.91-4.94]), and H. influenzae and M. catarrhalis (OR 2.34 [95%CI 1.40-3.91]), and a negative association was found between M. catarrhalis and S. aureus (OR 0.06 [95%CI 0.01-0.43]). Densities of S. pneumoniae, H. influenzae, and M. catarrhalis were positively correlated when two of these species were present. Prior to pneumococcal vaccine introduction, pneumococcal carriage prevalence and serotype distribution varies among children living in different regions of Indonesia. Positive associations in both carriage and density identified among S. pneumoniae, H. influenzae, and M. catarrhalis suggest a synergistic relationship among these species with potential clinical implications. |
| first_indexed | 2024-12-12T18:03:00Z |
| format | Article |
| id | doaj.art-0274aa15e65f41878ec98eb4446eb308 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T18:03:00Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0274aa15e65f41878ec98eb4446eb3082022-12-22T00:16:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01134e019509810.1371/journal.pone.0195098Carriage of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Staphylococcus aureus in Indonesian children: A cross-sectional study.Eileen M DunneChrysanti MuradSunaryati SudigdoadiEddy FadlyanaRodman TariganSang Ayu Kompiyang IndriyaniCasey L PellEmma WattsCatherine SatzkeJason HindsNurhandini Eka DewiFinny Fitry YaniKusnandi RusmilE Kim MulhollandCissy KartasasmitaStreptococcus pneumoniae is an important cause of infection and commonly colonizes the nasopharynx of young children, along with other potentially pathogenic bacteria. The objectives of this study were to estimate the carriage prevalence of S. pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Staphylococcus aureus in young children in Indonesia, and to examine interactions between these bacterial species. 302 healthy children aged 12-24 months were enrolled in community health centers in the Bandung, Central Lombok, and Padang regions. Nasopharyngeal swabs were collected and stored according to World Health Organization recommendations, and bacterial species detected by qPCR. Pneumococcal serotyping was conducted by microarray and latex agglutination/Quellung. Overall carriage prevalence was 49.5% for S. pneumoniae, 27.5% for H. influenzae, 42.7% for M. catarrhalis, and 7.3% for S. aureus. Prevalence of M. catarrhalis and S. pneumoniae, as well as pneumococcal serotype distribution, varied by region. Positive associations were observed for S. pneumoniae and M. catarrhalis (OR 3.07 [95%CI 1.91-4.94]), and H. influenzae and M. catarrhalis (OR 2.34 [95%CI 1.40-3.91]), and a negative association was found between M. catarrhalis and S. aureus (OR 0.06 [95%CI 0.01-0.43]). Densities of S. pneumoniae, H. influenzae, and M. catarrhalis were positively correlated when two of these species were present. Prior to pneumococcal vaccine introduction, pneumococcal carriage prevalence and serotype distribution varies among children living in different regions of Indonesia. Positive associations in both carriage and density identified among S. pneumoniae, H. influenzae, and M. catarrhalis suggest a synergistic relationship among these species with potential clinical implications.http://europepmc.org/articles/PMC5896896?pdf=render |
| spellingShingle | Eileen M Dunne Chrysanti Murad Sunaryati Sudigdoadi Eddy Fadlyana Rodman Tarigan Sang Ayu Kompiyang Indriyani Casey L Pell Emma Watts Catherine Satzke Jason Hinds Nurhandini Eka Dewi Finny Fitry Yani Kusnandi Rusmil E Kim Mulholland Cissy Kartasasmita Carriage of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Staphylococcus aureus in Indonesian children: A cross-sectional study. PLoS ONE |
| title | Carriage of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Staphylococcus aureus in Indonesian children: A cross-sectional study. |
| title_full | Carriage of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Staphylococcus aureus in Indonesian children: A cross-sectional study. |
| title_fullStr | Carriage of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Staphylococcus aureus in Indonesian children: A cross-sectional study. |
| title_full_unstemmed | Carriage of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Staphylococcus aureus in Indonesian children: A cross-sectional study. |
| title_short | Carriage of Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Staphylococcus aureus in Indonesian children: A cross-sectional study. |
| title_sort | carriage of streptococcus pneumoniae haemophilus influenzae moraxella catarrhalis and staphylococcus aureus in indonesian children a cross sectional study |
| url | http://europepmc.org/articles/PMC5896896?pdf=render |
| work_keys_str_mv | AT eileenmdunne carriageofstreptococcuspneumoniaehaemophilusinfluenzaemoraxellacatarrhalisandstaphylococcusaureusinindonesianchildrenacrosssectionalstudy AT chrysantimurad carriageofstreptococcuspneumoniaehaemophilusinfluenzaemoraxellacatarrhalisandstaphylococcusaureusinindonesianchildrenacrosssectionalstudy AT sunaryatisudigdoadi carriageofstreptococcuspneumoniaehaemophilusinfluenzaemoraxellacatarrhalisandstaphylococcusaureusinindonesianchildrenacrosssectionalstudy AT eddyfadlyana carriageofstreptococcuspneumoniaehaemophilusinfluenzaemoraxellacatarrhalisandstaphylococcusaureusinindonesianchildrenacrosssectionalstudy AT rodmantarigan carriageofstreptococcuspneumoniaehaemophilusinfluenzaemoraxellacatarrhalisandstaphylococcusaureusinindonesianchildrenacrosssectionalstudy AT sangayukompiyangindriyani carriageofstreptococcuspneumoniaehaemophilusinfluenzaemoraxellacatarrhalisandstaphylococcusaureusinindonesianchildrenacrosssectionalstudy AT caseylpell carriageofstreptococcuspneumoniaehaemophilusinfluenzaemoraxellacatarrhalisandstaphylococcusaureusinindonesianchildrenacrosssectionalstudy AT emmawatts carriageofstreptococcuspneumoniaehaemophilusinfluenzaemoraxellacatarrhalisandstaphylococcusaureusinindonesianchildrenacrosssectionalstudy AT catherinesatzke carriageofstreptococcuspneumoniaehaemophilusinfluenzaemoraxellacatarrhalisandstaphylococcusaureusinindonesianchildrenacrosssectionalstudy AT jasonhinds carriageofstreptococcuspneumoniaehaemophilusinfluenzaemoraxellacatarrhalisandstaphylococcusaureusinindonesianchildrenacrosssectionalstudy AT nurhandiniekadewi carriageofstreptococcuspneumoniaehaemophilusinfluenzaemoraxellacatarrhalisandstaphylococcusaureusinindonesianchildrenacrosssectionalstudy AT finnyfitryyani carriageofstreptococcuspneumoniaehaemophilusinfluenzaemoraxellacatarrhalisandstaphylococcusaureusinindonesianchildrenacrosssectionalstudy AT kusnandirusmil carriageofstreptococcuspneumoniaehaemophilusinfluenzaemoraxellacatarrhalisandstaphylococcusaureusinindonesianchildrenacrosssectionalstudy AT ekimmulholland carriageofstreptococcuspneumoniaehaemophilusinfluenzaemoraxellacatarrhalisandstaphylococcusaureusinindonesianchildrenacrosssectionalstudy AT cissykartasasmita carriageofstreptococcuspneumoniaehaemophilusinfluenzaemoraxellacatarrhalisandstaphylococcusaureusinindonesianchildrenacrosssectionalstudy |