Development of a novel plaque reduction neutralisation test for hantavirus infection
<p>In the Americas, hantaviruses cause severe cardiopulmonary syndrome (HCPS) with a high fatality rate. Hantavirus infection is commonly diagnosed using serologic techniques and reverse transcription-polymerase chain reaction. This paper presents a novel plaque reduction neutralisation test (...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2015-08-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000500624&lng=en&tlng=en |
| _version_ | 1797757295517499392 |
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| author | Michelly de Pádua William Marciel de Souza Flávio Lauretti Luiz Tadeu Moraes Figueiredo |
| author_facet | Michelly de Pádua William Marciel de Souza Flávio Lauretti Luiz Tadeu Moraes Figueiredo |
| author_sort | Michelly de Pádua |
| collection | DOAJ |
| description | <p>In the Americas, hantaviruses cause severe cardiopulmonary syndrome (HCPS) with a high fatality rate. Hantavirus infection is commonly diagnosed using serologic techniques and reverse transcription-polymerase chain reaction. This paper presents a novel plaque reduction neutralisation test (PRNT) for detecting antibodies to Brazilian hantavirus. Using PRNT, plaque detection was enhanced by adding 0.6% of dimethyl sulfoxide into the overlay culture medium of the infected cells. This procedure facilitated clear visualisation of small plaques under the microscope and provided for easy and accurate plaque counting. The sera from 37 HCPS patients from the city of Ribeirão Preto, Brazil was evaluated for the Rio Mamoré virus (RIOMV) using PRNT. Six samples exhibited neutralising antibodies; these antibodies exhibited a low titre. The low level of seropositive samples may be due to fewer cross-reactions between two different hantavirus species; the patients were likely infected by Araraquara virus (a virus that has not been isolated) and RIOMV was used for the test. This assay offers a new approach to evaluating and measuring neutralising antibodies produced during hantavirus infections and it can be adapted to other hantaviruses, including viruses that will be isolated in the future.</p> |
| first_indexed | 2024-03-12T18:13:51Z |
| format | Article |
| id | doaj.art-0276e17e6efb4ad49afd21c7adbe2641 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:13:51Z |
| publishDate | 2015-08-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-0276e17e6efb4ad49afd21c7adbe26412023-08-02T09:12:44ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602015-08-01110562462810.1590/0074-02760150102S0074-02762015000500624Development of a novel plaque reduction neutralisation test for hantavirus infectionMichelly de PáduaWilliam Marciel de SouzaFlávio LaurettiLuiz Tadeu Moraes Figueiredo<p>In the Americas, hantaviruses cause severe cardiopulmonary syndrome (HCPS) with a high fatality rate. Hantavirus infection is commonly diagnosed using serologic techniques and reverse transcription-polymerase chain reaction. This paper presents a novel plaque reduction neutralisation test (PRNT) for detecting antibodies to Brazilian hantavirus. Using PRNT, plaque detection was enhanced by adding 0.6% of dimethyl sulfoxide into the overlay culture medium of the infected cells. This procedure facilitated clear visualisation of small plaques under the microscope and provided for easy and accurate plaque counting. The sera from 37 HCPS patients from the city of Ribeirão Preto, Brazil was evaluated for the Rio Mamoré virus (RIOMV) using PRNT. Six samples exhibited neutralising antibodies; these antibodies exhibited a low titre. The low level of seropositive samples may be due to fewer cross-reactions between two different hantavirus species; the patients were likely infected by Araraquara virus (a virus that has not been isolated) and RIOMV was used for the test. This assay offers a new approach to evaluating and measuring neutralising antibodies produced during hantavirus infections and it can be adapted to other hantaviruses, including viruses that will be isolated in the future.</p>http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000500624&lng=en&tlng=enhantavirusplaque reduction neutralisation testcross-reaction in neutralisation testRio Mamoré virus |
| spellingShingle | Michelly de Pádua William Marciel de Souza Flávio Lauretti Luiz Tadeu Moraes Figueiredo Development of a novel plaque reduction neutralisation test for hantavirus infection Memorias do Instituto Oswaldo Cruz hantavirus plaque reduction neutralisation test cross-reaction in neutralisation test Rio Mamoré virus |
| title | Development of a novel plaque reduction neutralisation test for hantavirus infection |
| title_full | Development of a novel plaque reduction neutralisation test for hantavirus infection |
| title_fullStr | Development of a novel plaque reduction neutralisation test for hantavirus infection |
| title_full_unstemmed | Development of a novel plaque reduction neutralisation test for hantavirus infection |
| title_short | Development of a novel plaque reduction neutralisation test for hantavirus infection |
| title_sort | development of a novel plaque reduction neutralisation test for hantavirus infection |
| topic | hantavirus plaque reduction neutralisation test cross-reaction in neutralisation test Rio Mamoré virus |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015000500624&lng=en&tlng=en |
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