Spectrophotometric assay of creatinine in human serum sample
A new spectrophotometric method for the analysis of creatinine concentration in human serum samples is developed. The method explores the oxidation of p-methylamino phenol sulfate (Metol) in the presence of copper sulfate and creatinine which yields an intense violet colored species with maximum abs...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-05-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187853521300230X |
| _version_ | 1819085154489991168 |
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| author | Avinash Krishnegowda Nagaraja Padmarajaiah Shivakumar Anantharaman Krishna Honnur |
| author_facet | Avinash Krishnegowda Nagaraja Padmarajaiah Shivakumar Anantharaman Krishna Honnur |
| author_sort | Avinash Krishnegowda |
| collection | DOAJ |
| description | A new spectrophotometric method for the analysis of creatinine concentration in human serum samples is developed. The method explores the oxidation of p-methylamino phenol sulfate (Metol) in the presence of copper sulfate and creatinine which yields an intense violet colored species with maximum absorbance at 530 nm. The calibration graph of creatinine by fixed time assay ranged from 4.4 to 620 μM. Recovery of creatinine in human serum samples varied from 101% to 106%. Limit of detection and limit of quantification were 0.145 μM and 0.487 μM respectively. Sandell’s sensitivity was 0.112 μg cm−2 and molar absorptivity was 0.101 × 104 L mol−1 cm−1. Within day precision was 2.5–4.8% and day-to-day precision range was 3.2–7.8%. The robustness and ruggedness of the method expressed in RSD values ranged from 0.78% to 2.12% and 1.32% to 3.46% respectively, suggesting that the developed method was rugged. This method provides good sensitivity and is comparable to standard Jaffe’s method with comparatively less interference from foreign substances. |
| first_indexed | 2024-12-21T20:59:51Z |
| format | Article |
| id | doaj.art-027a8e95a7a24c228b622cf33af7add2 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-12-21T20:59:51Z |
| publishDate | 2017-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-027a8e95a7a24c228b622cf33af7add22022-12-21T18:50:27ZengElsevierArabian Journal of Chemistry1878-53522017-05-0110S2S2018S202410.1016/j.arabjc.2013.07.030Spectrophotometric assay of creatinine in human serum sampleAvinash Krishnegowda0Nagaraja Padmarajaiah1Shivakumar Anantharaman2Krishna Honnur3Department of Chemistry, ATME College of Engineering, Mysore 570028, IndiaDepartment of Studies in Chemistry, University of Mysore, Mysore 570006, IndiaDepartment of Chemistry, Regional Institute of Education, Mysore 570006, IndiaDepartment of Studies in Chemistry, University of Mysore, Mysore 570006, IndiaA new spectrophotometric method for the analysis of creatinine concentration in human serum samples is developed. The method explores the oxidation of p-methylamino phenol sulfate (Metol) in the presence of copper sulfate and creatinine which yields an intense violet colored species with maximum absorbance at 530 nm. The calibration graph of creatinine by fixed time assay ranged from 4.4 to 620 μM. Recovery of creatinine in human serum samples varied from 101% to 106%. Limit of detection and limit of quantification were 0.145 μM and 0.487 μM respectively. Sandell’s sensitivity was 0.112 μg cm−2 and molar absorptivity was 0.101 × 104 L mol−1 cm−1. Within day precision was 2.5–4.8% and day-to-day precision range was 3.2–7.8%. The robustness and ruggedness of the method expressed in RSD values ranged from 0.78% to 2.12% and 1.32% to 3.46% respectively, suggesting that the developed method was rugged. This method provides good sensitivity and is comparable to standard Jaffe’s method with comparatively less interference from foreign substances.http://www.sciencedirect.com/science/article/pii/S187853521300230XCreatinineMetolSerumJaffe’s |
| spellingShingle | Avinash Krishnegowda Nagaraja Padmarajaiah Shivakumar Anantharaman Krishna Honnur Spectrophotometric assay of creatinine in human serum sample Arabian Journal of Chemistry Creatinine Metol Serum Jaffe’s |
| title | Spectrophotometric assay of creatinine in human serum sample |
| title_full | Spectrophotometric assay of creatinine in human serum sample |
| title_fullStr | Spectrophotometric assay of creatinine in human serum sample |
| title_full_unstemmed | Spectrophotometric assay of creatinine in human serum sample |
| title_short | Spectrophotometric assay of creatinine in human serum sample |
| title_sort | spectrophotometric assay of creatinine in human serum sample |
| topic | Creatinine Metol Serum Jaffe’s |
| url | http://www.sciencedirect.com/science/article/pii/S187853521300230X |
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