Application on Gold Nanoparticles-Dotted 4-Nitrophenylazo Graphene in a Label-Free Impedimetric Deoxynivalenol Immunosensor
In this paper, we report a new concept to construct a label-free electrochemical inhibition-based immunosensor for the detection of the mycotoxin deoxynivalenol (DON) in cereal samples. The electrochemical impedance spectroscopy of tris(bipyridine) ruthenium (II) chloride was used as a marker enhan...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-02-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/15/2/3854 |
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| author | Christopher Edozie Sunday Milua Masikini Lindsay Wilson Candice Rassie Tesfaye Waryo Pricilla G. L. Baker Emmanuel I. Iwuoha |
| author_facet | Christopher Edozie Sunday Milua Masikini Lindsay Wilson Candice Rassie Tesfaye Waryo Pricilla G. L. Baker Emmanuel I. Iwuoha |
| author_sort | Christopher Edozie Sunday |
| collection | DOAJ |
| description | In this paper, we report a new concept to construct a label-free electrochemical inhibition-based immunosensor for the detection of the mycotoxin deoxynivalenol (DON) in cereal samples. The electrochemical impedance spectroscopy of tris(bipyridine) ruthenium (II) chloride was used as a marker enhanced with gold nanoparticles-dotted 4-nitrophenylazo functionalized graphene (AuNp/G/PhNO2) nanocatalyst mediated in Nafion on a glassy carbon electrode. Under the optimized conditions, the formation of immunocomplexes inhibited electron flow and increased the charge transfer resistance of the sensing interface linearly. The change in impedance was proportional to DON concentrations in the range of 6–30 ng/mL with a sensitivity and detection limit of 32.14 ΩL/ng and 0.3 µg/mL, respectively, which compares favorably with the ELISA result. The proposed sensor had a stability of 80.3%, good precision and selectivity in DON standard solution containing different interfering agents, indicating promising application prospect for this strategy in designing impedimetric, electrochemiluminescent, voltammetric or amperometric sensors. |
| first_indexed | 2024-04-11T22:30:45Z |
| format | Article |
| id | doaj.art-027b0fab76824897804dd6f359de3ffb |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T22:30:45Z |
| publishDate | 2015-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-027b0fab76824897804dd6f359de3ffb2022-12-22T03:59:24ZengMDPI AGSensors1424-82202015-02-011523854387110.3390/s150203854s150203854Application on Gold Nanoparticles-Dotted 4-Nitrophenylazo Graphene in a Label-Free Impedimetric Deoxynivalenol ImmunosensorChristopher Edozie Sunday0Milua Masikini1Lindsay Wilson2Candice Rassie3Tesfaye Waryo4Pricilla G. L. Baker5Emmanuel I. Iwuoha6Sensor Laboratory, Chemistry Department, University of the Western Cape, Private Bag X 17, Bellville 7535, South AfricaSensor Laboratory, Chemistry Department, University of the Western Cape, Private Bag X 17, Bellville 7535, South AfricaSensor Laboratory, Chemistry Department, University of the Western Cape, Private Bag X 17, Bellville 7535, South AfricaSensor Laboratory, Chemistry Department, University of the Western Cape, Private Bag X 17, Bellville 7535, South AfricaSensor Laboratory, Chemistry Department, University of the Western Cape, Private Bag X 17, Bellville 7535, South AfricaSensor Laboratory, Chemistry Department, University of the Western Cape, Private Bag X 17, Bellville 7535, South AfricaSensor Laboratory, Chemistry Department, University of the Western Cape, Private Bag X 17, Bellville 7535, South AfricaIn this paper, we report a new concept to construct a label-free electrochemical inhibition-based immunosensor for the detection of the mycotoxin deoxynivalenol (DON) in cereal samples. The electrochemical impedance spectroscopy of tris(bipyridine) ruthenium (II) chloride was used as a marker enhanced with gold nanoparticles-dotted 4-nitrophenylazo functionalized graphene (AuNp/G/PhNO2) nanocatalyst mediated in Nafion on a glassy carbon electrode. Under the optimized conditions, the formation of immunocomplexes inhibited electron flow and increased the charge transfer resistance of the sensing interface linearly. The change in impedance was proportional to DON concentrations in the range of 6–30 ng/mL with a sensitivity and detection limit of 32.14 ΩL/ng and 0.3 µg/mL, respectively, which compares favorably with the ELISA result. The proposed sensor had a stability of 80.3%, good precision and selectivity in DON standard solution containing different interfering agents, indicating promising application prospect for this strategy in designing impedimetric, electrochemiluminescent, voltammetric or amperometric sensors.http://www.mdpi.com/1424-8220/15/2/3854deoxynivalenolmycotoxingrapheneimmunosensorgraphene oxideNafion |
| spellingShingle | Christopher Edozie Sunday Milua Masikini Lindsay Wilson Candice Rassie Tesfaye Waryo Pricilla G. L. Baker Emmanuel I. Iwuoha Application on Gold Nanoparticles-Dotted 4-Nitrophenylazo Graphene in a Label-Free Impedimetric Deoxynivalenol Immunosensor Sensors deoxynivalenol mycotoxin graphene immunosensor graphene oxide Nafion |
| title | Application on Gold Nanoparticles-Dotted 4-Nitrophenylazo Graphene in a Label-Free Impedimetric Deoxynivalenol Immunosensor |
| title_full | Application on Gold Nanoparticles-Dotted 4-Nitrophenylazo Graphene in a Label-Free Impedimetric Deoxynivalenol Immunosensor |
| title_fullStr | Application on Gold Nanoparticles-Dotted 4-Nitrophenylazo Graphene in a Label-Free Impedimetric Deoxynivalenol Immunosensor |
| title_full_unstemmed | Application on Gold Nanoparticles-Dotted 4-Nitrophenylazo Graphene in a Label-Free Impedimetric Deoxynivalenol Immunosensor |
| title_short | Application on Gold Nanoparticles-Dotted 4-Nitrophenylazo Graphene in a Label-Free Impedimetric Deoxynivalenol Immunosensor |
| title_sort | application on gold nanoparticles dotted 4 nitrophenylazo graphene in a label free impedimetric deoxynivalenol immunosensor |
| topic | deoxynivalenol mycotoxin graphene immunosensor graphene oxide Nafion |
| url | http://www.mdpi.com/1424-8220/15/2/3854 |
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