Early Response of Antimicrobial Resistance and Virulence Genes Expression in Classical, Hypervirulent, and Hybrid hvKp-MDR <i>Klebsiella pneumoniae</i> on Antimicrobial Stress
<i>Klebsiella pneumoniae</i> is an increasingly important hospital pathogen. Classical <i>K. pneumoniae</i> (cKp) and hypervirulent <i>K. pneumoniae</i> (hvKp) are two distinct evolutionary genetic lines. The recently ongoing evolution of <i>K. pneumoniae<...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-12-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/11/1/7 |
| _version_ | 1827667061431599104 |
|---|---|
| author | Anastasiia D. Fursova Mikhail V. Fursov Evgenii I. Astashkin Tatiana S. Novikova Galina N. Fedyukina Angelina A. Kislichkina Irina A. Alexandrova Olga N. Ershova Ivan A. Dyatlov Nadezhda K. Fursova |
| author_facet | Anastasiia D. Fursova Mikhail V. Fursov Evgenii I. Astashkin Tatiana S. Novikova Galina N. Fedyukina Angelina A. Kislichkina Irina A. Alexandrova Olga N. Ershova Ivan A. Dyatlov Nadezhda K. Fursova |
| author_sort | Anastasiia D. Fursova |
| collection | DOAJ |
| description | <i>Klebsiella pneumoniae</i> is an increasingly important hospital pathogen. Classical <i>K. pneumoniae</i> (cKp) and hypervirulent <i>K. pneumoniae</i> (hvKp) are two distinct evolutionary genetic lines. The recently ongoing evolution of <i>K. pneumoniae</i> resulted in the generation of hybrid hvKP-MDR strains. <i>K. pneumoniae</i> distinct isolates (<i>n</i> = 70) belonged to 20 sequence types with the prevalence of ST395 (27.1%), ST23 (18.6%), ST147 (15.7%), and ST86 (7.1%), and 17 capsular types with the predominance of K2 (31.4%), K57 (18.6%), K64 (10.0%), K1 (5.7%) were isolated from patients of the Moscow neurosurgery ICU in 2014–2019. The rate of multi-drug resistant (MDR) and carbapenem-resistant phenotypes were 84.3% and 45.7%, respectively. Whole-genome sequencing of five selected strains belonging to cKp (ST395<sup>K47</sup> and ST147<sup>K64</sup>), hvKp (ST86<sup>K2</sup>), and hvKp-MDR (ST23<sup>K1</sup> and ST23<sup>K57</sup>) revealed <i>bla</i><sub>SHV</sub>, <i>bla</i><sub>TEM</sub>, <i>bla</i><sub>CTX</sub>, <i>bla</i><sub>OXA-48</sub>, and <i>bla</i><sub>NDM</sub> beta-lactamase genes; <i>acr</i>, <i>oqx</i>, <i>kpn</i>, <i>kde</i>, and <i>kex</i> efflux genes; and <i>K. pneumoniae</i> virulence genes. Selective pressure of 100 mg/L ampicillin or 10 mg/L ceftriaxone induced changes of expression levels for named genes in the strains belonging to cKp, hvKp, and hybrid hvKp-MDR. Obtained results seem to be important for epidemiologists and clinicians for enhancing knowledge about hospital pathogens. |
| first_indexed | 2024-03-10T03:02:20Z |
| format | Article |
| id | doaj.art-027c169deae94fe78b241887c5ceaf05 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T03:02:20Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-027c169deae94fe78b241887c5ceaf052023-11-23T12:42:31ZengMDPI AGAntibiotics2079-63822021-12-01111710.3390/antibiotics11010007Early Response of Antimicrobial Resistance and Virulence Genes Expression in Classical, Hypervirulent, and Hybrid hvKp-MDR <i>Klebsiella pneumoniae</i> on Antimicrobial StressAnastasiia D. Fursova0Mikhail V. Fursov1Evgenii I. Astashkin2Tatiana S. Novikova3Galina N. Fedyukina4Angelina A. Kislichkina5Irina A. Alexandrova6Olga N. Ershova7Ivan A. Dyatlov8Nadezhda K. Fursova9State Research Center for Applied Microbiology and Biotechnology, Territory “Kvartal A”, 142279 Obolensk, Moscow Region, RussiaState Research Center for Applied Microbiology and Biotechnology, Territory “Kvartal A”, 142279 Obolensk, Moscow Region, RussiaState Research Center for Applied Microbiology and Biotechnology, Territory “Kvartal A”, 142279 Obolensk, Moscow Region, RussiaState Research Center for Applied Microbiology and Biotechnology, Territory “Kvartal A”, 142279 Obolensk, Moscow Region, RussiaState Research Center for Applied Microbiology and Biotechnology, Territory “Kvartal A”, 142279 Obolensk, Moscow Region, RussiaState Research Center for Applied Microbiology and Biotechnology, Territory “Kvartal A”, 142279 Obolensk, Moscow Region, RussiaNational Medical Research Center of Neurosurgery Named after Academician N.N. Burdenko, 125047 Moscow, RussiaNational Medical Research Center of Neurosurgery Named after Academician N.N. Burdenko, 125047 Moscow, RussiaState Research Center for Applied Microbiology and Biotechnology, Territory “Kvartal A”, 142279 Obolensk, Moscow Region, RussiaState Research Center for Applied Microbiology and Biotechnology, Territory “Kvartal A”, 142279 Obolensk, Moscow Region, Russia<i>Klebsiella pneumoniae</i> is an increasingly important hospital pathogen. Classical <i>K. pneumoniae</i> (cKp) and hypervirulent <i>K. pneumoniae</i> (hvKp) are two distinct evolutionary genetic lines. The recently ongoing evolution of <i>K. pneumoniae</i> resulted in the generation of hybrid hvKP-MDR strains. <i>K. pneumoniae</i> distinct isolates (<i>n</i> = 70) belonged to 20 sequence types with the prevalence of ST395 (27.1%), ST23 (18.6%), ST147 (15.7%), and ST86 (7.1%), and 17 capsular types with the predominance of K2 (31.4%), K57 (18.6%), K64 (10.0%), K1 (5.7%) were isolated from patients of the Moscow neurosurgery ICU in 2014–2019. The rate of multi-drug resistant (MDR) and carbapenem-resistant phenotypes were 84.3% and 45.7%, respectively. Whole-genome sequencing of five selected strains belonging to cKp (ST395<sup>K47</sup> and ST147<sup>K64</sup>), hvKp (ST86<sup>K2</sup>), and hvKp-MDR (ST23<sup>K1</sup> and ST23<sup>K57</sup>) revealed <i>bla</i><sub>SHV</sub>, <i>bla</i><sub>TEM</sub>, <i>bla</i><sub>CTX</sub>, <i>bla</i><sub>OXA-48</sub>, and <i>bla</i><sub>NDM</sub> beta-lactamase genes; <i>acr</i>, <i>oqx</i>, <i>kpn</i>, <i>kde</i>, and <i>kex</i> efflux genes; and <i>K. pneumoniae</i> virulence genes. Selective pressure of 100 mg/L ampicillin or 10 mg/L ceftriaxone induced changes of expression levels for named genes in the strains belonging to cKp, hvKp, and hybrid hvKp-MDR. Obtained results seem to be important for epidemiologists and clinicians for enhancing knowledge about hospital pathogens.https://www.mdpi.com/2079-6382/11/1/7Klebsiella pneumoniaesequence typecapsular typeMDRhvKphybrid hvKp-MDR |
| spellingShingle | Anastasiia D. Fursova Mikhail V. Fursov Evgenii I. Astashkin Tatiana S. Novikova Galina N. Fedyukina Angelina A. Kislichkina Irina A. Alexandrova Olga N. Ershova Ivan A. Dyatlov Nadezhda K. Fursova Early Response of Antimicrobial Resistance and Virulence Genes Expression in Classical, Hypervirulent, and Hybrid hvKp-MDR <i>Klebsiella pneumoniae</i> on Antimicrobial Stress Antibiotics Klebsiella pneumoniae sequence type capsular type MDR hvKp hybrid hvKp-MDR |
| title | Early Response of Antimicrobial Resistance and Virulence Genes Expression in Classical, Hypervirulent, and Hybrid hvKp-MDR <i>Klebsiella pneumoniae</i> on Antimicrobial Stress |
| title_full | Early Response of Antimicrobial Resistance and Virulence Genes Expression in Classical, Hypervirulent, and Hybrid hvKp-MDR <i>Klebsiella pneumoniae</i> on Antimicrobial Stress |
| title_fullStr | Early Response of Antimicrobial Resistance and Virulence Genes Expression in Classical, Hypervirulent, and Hybrid hvKp-MDR <i>Klebsiella pneumoniae</i> on Antimicrobial Stress |
| title_full_unstemmed | Early Response of Antimicrobial Resistance and Virulence Genes Expression in Classical, Hypervirulent, and Hybrid hvKp-MDR <i>Klebsiella pneumoniae</i> on Antimicrobial Stress |
| title_short | Early Response of Antimicrobial Resistance and Virulence Genes Expression in Classical, Hypervirulent, and Hybrid hvKp-MDR <i>Klebsiella pneumoniae</i> on Antimicrobial Stress |
| title_sort | early response of antimicrobial resistance and virulence genes expression in classical hypervirulent and hybrid hvkp mdr i klebsiella pneumoniae i on antimicrobial stress |
| topic | Klebsiella pneumoniae sequence type capsular type MDR hvKp hybrid hvKp-MDR |
| url | https://www.mdpi.com/2079-6382/11/1/7 |
| work_keys_str_mv | AT anastasiiadfursova earlyresponseofantimicrobialresistanceandvirulencegenesexpressioninclassicalhypervirulentandhybridhvkpmdriklebsiellapneumoniaeionantimicrobialstress AT mikhailvfursov earlyresponseofantimicrobialresistanceandvirulencegenesexpressioninclassicalhypervirulentandhybridhvkpmdriklebsiellapneumoniaeionantimicrobialstress AT evgeniiiastashkin earlyresponseofantimicrobialresistanceandvirulencegenesexpressioninclassicalhypervirulentandhybridhvkpmdriklebsiellapneumoniaeionantimicrobialstress AT tatianasnovikova earlyresponseofantimicrobialresistanceandvirulencegenesexpressioninclassicalhypervirulentandhybridhvkpmdriklebsiellapneumoniaeionantimicrobialstress AT galinanfedyukina earlyresponseofantimicrobialresistanceandvirulencegenesexpressioninclassicalhypervirulentandhybridhvkpmdriklebsiellapneumoniaeionantimicrobialstress AT angelinaakislichkina earlyresponseofantimicrobialresistanceandvirulencegenesexpressioninclassicalhypervirulentandhybridhvkpmdriklebsiellapneumoniaeionantimicrobialstress AT irinaaalexandrova earlyresponseofantimicrobialresistanceandvirulencegenesexpressioninclassicalhypervirulentandhybridhvkpmdriklebsiellapneumoniaeionantimicrobialstress AT olganershova earlyresponseofantimicrobialresistanceandvirulencegenesexpressioninclassicalhypervirulentandhybridhvkpmdriklebsiellapneumoniaeionantimicrobialstress AT ivanadyatlov earlyresponseofantimicrobialresistanceandvirulencegenesexpressioninclassicalhypervirulentandhybridhvkpmdriklebsiellapneumoniaeionantimicrobialstress AT nadezhdakfursova earlyresponseofantimicrobialresistanceandvirulencegenesexpressioninclassicalhypervirulentandhybridhvkpmdriklebsiellapneumoniaeionantimicrobialstress |