<i>TaDrAp1</i> and <i>TaDrAp2</i>, Partner Genes of a Transcription Repressor, Coordinate Plant Development and Drought Tolerance in Spelt and Bread Wheat

<i>TaDrAp1</i> and <i>TaDrAp2</i>, Partner Genes of a Transcription Repressor, Coordinate Plant Development and Drought Tolerance in Spelt and Bread Wheat

Down-regulator associated protein, DrAp1, acts as a negative cofactor (NC2α) in a transcription repressor complex together with another subunit, down-regulator Dr1 (NC2β). In binding to promotors and regulating the initiation of transcription of various genes, <i>DrAp1</i> plays a key ro...

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Bibliographic Details
Main Authors: Lyudmila Zotova, Nasgul Shamambaeva, Katso Lethola, Badr Alharthi, Valeriya Vavilova, Svetlana E. Smolenskaya, Nikolay P. Goncharov, Akhylbek Kurishbayev, Satyvaldy Jatayev, Narendra K. Gupta, Sunita Gupta, Carly Schramm, Peter A. Anderson, Colin L. D. Jenkins, Kathleen L. Soole, Yuri Shavrukov
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:International Journal of Molecular Sciences
Subjects:
down-regulator associated protein (DrAp1)
drought
gene expression
microarray 40K SNP assay
negative cofactor 2 (NC2)
nuclear factor Y (NF-Y)
Online Access:https://www.mdpi.com/1422-0067/21/21/8296
Description
Summary:Down-regulator associated protein, DrAp1, acts as a negative cofactor (NC2α) in a transcription repressor complex together with another subunit, down-regulator Dr1 (NC2β). In binding to promotors and regulating the initiation of transcription of various genes, <i>DrAp1</i> plays a key role in plant transition to flowering and ultimately in seed production. <i>TaDrAp1</i> and <i>TaDrAp2</i> genes were identified, and their expression and genetic polymorphism were studied using bioinformatics, qPCR analyses, a 40K Single nucleotide polymorphism (SNP) microarray, and Amplifluor-like SNP genotyping in cultivars of bread wheat (<i>Triticum aestivum</i> L.) and breeding lines developed from a cross between spelt (<i>T. spelta</i> L.) and bread wheat. <i>TaDrAp1</i> was highly expressed under non-stressed conditions, and at flowering, <i>TaDrAp1</i> expression was negatively correlated with yield capacity. <i>TaDrAp2</i> showed a consistently low level of mRNA production. Drought caused changes in the expression of both <i>TaDrAp1</i> and <i>TaDrAp2</i> genes in opposite directions, effectively increasing expression in lower yielding cultivars. The microarray 40K SNP assay and Amplifluor-like SNP marker, revealed clear scores and allele discriminations for <i>TaDrAp1</i> and <i>TaDrAp2</i> and <i>TaRht-B1</i> genes. Alleles of two particular homeologs, <i>TaDrAp1-B4</i> and <i>TaDrAp2-B1</i>, co-segregated with grain yield in nine selected breeding lines. This indicated an important regulatory role for both <i>TaDrAp1</i> and <i>TaDrAp2</i> genes in plant growth, ontogenesis, and drought tolerance in bread and spelt wheat.
ISSN:1661-6596
1422-0067

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