<i>TaDrAp1</i> and <i>TaDrAp2</i>, Partner Genes of a Transcription Repressor, Coordinate Plant Development and Drought Tolerance in Spelt and Bread Wheat
Down-regulator associated protein, DrAp1, acts as a negative cofactor (NC2α) in a transcription repressor complex together with another subunit, down-regulator Dr1 (NC2β). In binding to promotors and regulating the initiation of transcription of various genes, <i>DrAp1</i> plays a key ro...
Main Authors: | , , , , , , , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-11-01
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Series: | International Journal of Molecular Sciences |
Subjects: | |
Online Access: | https://www.mdpi.com/1422-0067/21/21/8296 |
Summary: | Down-regulator associated protein, DrAp1, acts as a negative cofactor (NC2α) in a transcription repressor complex together with another subunit, down-regulator Dr1 (NC2β). In binding to promotors and regulating the initiation of transcription of various genes, <i>DrAp1</i> plays a key role in plant transition to flowering and ultimately in seed production. <i>TaDrAp1</i> and <i>TaDrAp2</i> genes were identified, and their expression and genetic polymorphism were studied using bioinformatics, qPCR analyses, a 40K Single nucleotide polymorphism (SNP) microarray, and Amplifluor-like SNP genotyping in cultivars of bread wheat (<i>Triticum aestivum</i> L.) and breeding lines developed from a cross between spelt (<i>T. spelta</i> L.) and bread wheat. <i>TaDrAp1</i> was highly expressed under non-stressed conditions, and at flowering, <i>TaDrAp1</i> expression was negatively correlated with yield capacity. <i>TaDrAp2</i> showed a consistently low level of mRNA production. Drought caused changes in the expression of both <i>TaDrAp1</i> and <i>TaDrAp2</i> genes in opposite directions, effectively increasing expression in lower yielding cultivars. The microarray 40K SNP assay and Amplifluor-like SNP marker, revealed clear scores and allele discriminations for <i>TaDrAp1</i> and <i>TaDrAp2</i> and <i>TaRht-B1</i> genes. Alleles of two particular homeologs, <i>TaDrAp1-B4</i> and <i>TaDrAp2-B1</i>, co-segregated with grain yield in nine selected breeding lines. This indicated an important regulatory role for both <i>TaDrAp1</i> and <i>TaDrAp2</i> genes in plant growth, ontogenesis, and drought tolerance in bread and spelt wheat. |
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ISSN: | 1661-6596 1422-0067 |