Silver nanoparticles biosynthesis using an airborne fungal isolate, Aspergillus flavus: optimization, characterization and antibacterial activity
Background and Objectives: Nanoscience is one of the most important branches of modern science, which deals with the knowledge, structure, and properties of nanoparticles. This study aimed to investigate the ability of an airborne fungus (Aspergillus flavus) to synthesize silver nanoparticles (AgNP...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2022-08-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/3589 |
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| author | Aram Al-Soub Khaled Khleifat Amjad Al-Tarawneh Muhamad Al-Limoun Ibrahim Alfarrayeh Ahmad Al Sarayreh Yaseen Al Qaisi Haitham Qaralleh Moath Alqaraleh Anas Albashaireh |
| author_facet | Aram Al-Soub Khaled Khleifat Amjad Al-Tarawneh Muhamad Al-Limoun Ibrahim Alfarrayeh Ahmad Al Sarayreh Yaseen Al Qaisi Haitham Qaralleh Moath Alqaraleh Anas Albashaireh |
| author_sort | Aram Al-Soub |
| collection | DOAJ |
| description |
Background and Objectives: Nanoscience is one of the most important branches of modern science, which deals with the knowledge, structure, and properties of nanoparticles. This study aimed to investigate the ability of an airborne fungus (Aspergillus flavus) to synthesize silver nanoparticles (AgNPs) and to test the antibacterial activity of the synthesized AgNPs.
Materials and Methods: The confirmation of AgNPs synthesis and the characterization of their properties were done using UV-Vis spectrophotometer, Zeta potential, Zeta sizer, FT-IR, and XRD analyses. The antibacterial activity was determined using broth microdilution method.
Results: The findings showed that the average diameter of the resultant AgNPs was 474.2 nm with a PDI value of 0.27, and the zeta potential was -33.8 mV. Transmission electron microscopy (TEM) revealed that the AgNPs were regular and spherical in shape. TEM micrographs demonstrated that the AgNPs were smaller than those that were observed by DLS examination because the drying process resulted in particle shrinkage. The average size of AgNPs were less than 35 nm. The AgNPs exhibited a remarkable antibacterial activity against K. pneumoniae, E. coli, E. cloacae, S. aureus, S. epidermidis, and Shigella sp., and the MIC values ranged from 25 to 100 µg/mL. However, an exception was P. aeruginosa in which its MIC was >125 µg/mL.
Conclusion: The results suggest that, the biosynthesized AgNPs by A. flavus could be utilized as a source of potent antibacterial agents in medicine and biotechnological applications.
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| first_indexed | 2024-04-13T02:16:34Z |
| format | Article |
| id | doaj.art-027f1c457de340eab1d20da0690ad917 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-04-13T02:16:34Z |
| publishDate | 2022-08-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-027f1c457de340eab1d20da0690ad9172022-12-22T03:07:07ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472022-08-0114410.18502/ijm.v14i4.10238Silver nanoparticles biosynthesis using an airborne fungal isolate, Aspergillus flavus: optimization, characterization and antibacterial activityAram Al-Soub0Khaled Khleifat1Amjad Al-Tarawneh2Muhamad Al-Limoun3Ibrahim Alfarrayeh4Ahmad Al Sarayreh5Yaseen Al Qaisi6Haitham Qaralleh7Moath Alqaraleh8Anas Albashaireh9Department of Biological Sciences, Faculty of Science, Mutah University, Al-Karak, JordanDepartment of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman, Jordanrince Faisal Center for Dead Sea, Environmental and Energy Research, Mutah University, Mutah, Karak, JordanDepartment of Biological Sciences, Faculty of Science, Mutah University, Al-Karak, JordanDepartment of Biological Sciences, Faculty of Science, Mutah University, Al-Karak, JordanDepartment of Biological Sciences, Faculty of Science, Mutah University, Al-Karak, JordanDepartment of Biological Sciences, Faculty of Science, Mutah University, Al-Karak, JordanDepartment of Medical Laboratory Sciences, Faculty of Science, Mutah University, Al-Karak, JordanPharmacological and Diagnostic Research Center (PDRC), Faculty of Pharmacy, Al-Ahliyya Amman University, Amman, JordanDepartment of Biological Sciences, Faculty of Science, Mutah University, Al-Karak, Jordan Background and Objectives: Nanoscience is one of the most important branches of modern science, which deals with the knowledge, structure, and properties of nanoparticles. This study aimed to investigate the ability of an airborne fungus (Aspergillus flavus) to synthesize silver nanoparticles (AgNPs) and to test the antibacterial activity of the synthesized AgNPs. Materials and Methods: The confirmation of AgNPs synthesis and the characterization of their properties were done using UV-Vis spectrophotometer, Zeta potential, Zeta sizer, FT-IR, and XRD analyses. The antibacterial activity was determined using broth microdilution method. Results: The findings showed that the average diameter of the resultant AgNPs was 474.2 nm with a PDI value of 0.27, and the zeta potential was -33.8 mV. Transmission electron microscopy (TEM) revealed that the AgNPs were regular and spherical in shape. TEM micrographs demonstrated that the AgNPs were smaller than those that were observed by DLS examination because the drying process resulted in particle shrinkage. The average size of AgNPs were less than 35 nm. The AgNPs exhibited a remarkable antibacterial activity against K. pneumoniae, E. coli, E. cloacae, S. aureus, S. epidermidis, and Shigella sp., and the MIC values ranged from 25 to 100 µg/mL. However, an exception was P. aeruginosa in which its MIC was >125 µg/mL. Conclusion: The results suggest that, the biosynthesized AgNPs by A. flavus could be utilized as a source of potent antibacterial agents in medicine and biotechnological applications. https://ijm.tums.ac.ir/index.php/ijm/article/view/3589Nanoparticles;Fungi;Antibacterial agents;Nanomedicine;Aspergillus flavus |
| spellingShingle | Aram Al-Soub Khaled Khleifat Amjad Al-Tarawneh Muhamad Al-Limoun Ibrahim Alfarrayeh Ahmad Al Sarayreh Yaseen Al Qaisi Haitham Qaralleh Moath Alqaraleh Anas Albashaireh Silver nanoparticles biosynthesis using an airborne fungal isolate, Aspergillus flavus: optimization, characterization and antibacterial activity Iranian Journal of Microbiology Nanoparticles; Fungi; Antibacterial agents; Nanomedicine; Aspergillus flavus |
| title | Silver nanoparticles biosynthesis using an airborne fungal isolate, Aspergillus flavus: optimization, characterization and antibacterial activity |
| title_full | Silver nanoparticles biosynthesis using an airborne fungal isolate, Aspergillus flavus: optimization, characterization and antibacterial activity |
| title_fullStr | Silver nanoparticles biosynthesis using an airborne fungal isolate, Aspergillus flavus: optimization, characterization and antibacterial activity |
| title_full_unstemmed | Silver nanoparticles biosynthesis using an airborne fungal isolate, Aspergillus flavus: optimization, characterization and antibacterial activity |
| title_short | Silver nanoparticles biosynthesis using an airborne fungal isolate, Aspergillus flavus: optimization, characterization and antibacterial activity |
| title_sort | silver nanoparticles biosynthesis using an airborne fungal isolate aspergillus flavus optimization characterization and antibacterial activity |
| topic | Nanoparticles; Fungi; Antibacterial agents; Nanomedicine; Aspergillus flavus |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/3589 |
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