Intermittent hilar occlusion attenuates or prevents renal ischaemia-reperfusion in mice
Background: A minimal decrease in renal function in partial nephrectomy is an important clinical and experimental research objective. We compared the effect of intermittent hilar occlusion (IHO) and continuous hilar occlusion (CHO) on kidney injury and oxidative damage. Method: Using CHO or IHO for...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332222008460 |
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| author | Lei Wang Cheng Peng Jianwen Chen Huaikang Li Qilong Jiao Zhou Zhang Li Wang Qing Yuan Baojun Wang Yan Huang Xin Ma |
| author_facet | Lei Wang Cheng Peng Jianwen Chen Huaikang Li Qilong Jiao Zhou Zhang Li Wang Qing Yuan Baojun Wang Yan Huang Xin Ma |
| author_sort | Lei Wang |
| collection | DOAJ |
| description | Background: A minimal decrease in renal function in partial nephrectomy is an important clinical and experimental research objective. We compared the effect of intermittent hilar occlusion (IHO) and continuous hilar occlusion (CHO) on kidney injury and oxidative damage. Method: Using CHO or IHO for 24, 30, and 45 min, intraoperative ischaemia, as well as a sham group, were set up in an ischaemia-reperfusion long-term survival mouse model. Renal function, pathological injury, subcellular structure injury, and cell apoptosis were evaluated at 1, 30, and 90 d postoperatively to study the protective effect of IHO. To do so, oxidative damage analysis, antioxidant activities, and ischaemia time dose-response curves on relative acute renal function injury were analysed. Results: All parameters indicated that kidney injury was dramatically attenuated in the IHO groups compared to those in the corresponding CHO groups. Particularly, mice in the IHO 24-min group incurred no tubular injury or renal functional injury; mice in the CHO 30-min group incurred severe acute and chronic kidney injury, but mice in the IHO 30-min group only experienced transient mild renal functional injury. IHO prolonged tolerable ischaemia duration. Conclusions: IHO can prolong the duration of tolerable ischaemia, at least partly through protecting the kidney from oxidative stress, consequently attenuating or preventing renal ischaemia-reperfusion injury. Reduced kidney injury after ischaemia-reperfusion is linked to a greater maintenance of antioxidant activity and less oxidative damage. The protective effect of IHO can be extremely efficient if properly applied; IHO may avoid renal ischaemia-reperfusion injury during partial nephrectomy. |
| first_indexed | 2024-04-11T14:14:12Z |
| format | Article |
| id | doaj.art-02811394f5314ba29ccfa4de93715a60 |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-04-11T14:14:12Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-02811394f5314ba29ccfa4de93715a602022-12-22T04:19:36ZengElsevierBiomedicine & Pharmacotherapy0753-33222022-09-01153113457Intermittent hilar occlusion attenuates or prevents renal ischaemia-reperfusion in miceLei Wang0Cheng Peng1Jianwen Chen2Huaikang Li3Qilong Jiao4Zhou Zhang5Li Wang6Qing Yuan7Baojun Wang8Yan Huang9Xin Ma10Senior Department of Urology, the Third Medical Centre of PLA General Hospital, Beijing 100039, China; Department of Urology, Chinese PLA 96606 Military Hospital, Luoyang 471003, ChinaSenior Department of Urology, the Third Medical Centre of PLA General Hospital, Beijing 100039, ChinaDepartment of Nephrology, the First Medical Centre, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Centre for Kidney Diseases, Beijing Key Laboratory of Kidney Diseases, Beijing 100853, ChinaSenior Department of Urology, the Third Medical Centre of PLA General Hospital, Beijing 100039, ChinaSenior Department of Urology, the Third Medical Centre of PLA General Hospital, Beijing 100039, ChinaDepartment of Nephrology, the First Medical Centre, Chinese PLA General Hospital, Chinese PLA Institute of Nephrology, State Key Laboratory of Kidney Diseases, National Clinical Research Centre for Kidney Diseases, Beijing Key Laboratory of Kidney Diseases, Beijing 100853, China; Department of Nephrology, Characteristic Medical Centre of the Chinese People's Armed Police Force, Tianjin 300160, ChinaSenior Department of Urology, the Third Medical Centre of PLA General Hospital, Beijing 100039, ChinaSenior Department of Urology, the Third Medical Centre of PLA General Hospital, Beijing 100039, ChinaSenior Department of Urology, the Third Medical Centre of PLA General Hospital, Beijing 100039, ChinaSenior Department of Urology, the Third Medical Centre of PLA General Hospital, Beijing 100039, ChinaSenior Department of Urology, the Third Medical Centre of PLA General Hospital, Beijing 100039, China; Corresponding author.Background: A minimal decrease in renal function in partial nephrectomy is an important clinical and experimental research objective. We compared the effect of intermittent hilar occlusion (IHO) and continuous hilar occlusion (CHO) on kidney injury and oxidative damage. Method: Using CHO or IHO for 24, 30, and 45 min, intraoperative ischaemia, as well as a sham group, were set up in an ischaemia-reperfusion long-term survival mouse model. Renal function, pathological injury, subcellular structure injury, and cell apoptosis were evaluated at 1, 30, and 90 d postoperatively to study the protective effect of IHO. To do so, oxidative damage analysis, antioxidant activities, and ischaemia time dose-response curves on relative acute renal function injury were analysed. Results: All parameters indicated that kidney injury was dramatically attenuated in the IHO groups compared to those in the corresponding CHO groups. Particularly, mice in the IHO 24-min group incurred no tubular injury or renal functional injury; mice in the CHO 30-min group incurred severe acute and chronic kidney injury, but mice in the IHO 30-min group only experienced transient mild renal functional injury. IHO prolonged tolerable ischaemia duration. Conclusions: IHO can prolong the duration of tolerable ischaemia, at least partly through protecting the kidney from oxidative stress, consequently attenuating or preventing renal ischaemia-reperfusion injury. Reduced kidney injury after ischaemia-reperfusion is linked to a greater maintenance of antioxidant activity and less oxidative damage. The protective effect of IHO can be extremely efficient if properly applied; IHO may avoid renal ischaemia-reperfusion injury during partial nephrectomy.http://www.sciencedirect.com/science/article/pii/S0753332222008460Partial nephrectomyIschaemia-reperfusion injuryOxidative damageLaboratory animal models |
| spellingShingle | Lei Wang Cheng Peng Jianwen Chen Huaikang Li Qilong Jiao Zhou Zhang Li Wang Qing Yuan Baojun Wang Yan Huang Xin Ma Intermittent hilar occlusion attenuates or prevents renal ischaemia-reperfusion in mice Biomedicine & Pharmacotherapy Partial nephrectomy Ischaemia-reperfusion injury Oxidative damage Laboratory animal models |
| title | Intermittent hilar occlusion attenuates or prevents renal ischaemia-reperfusion in mice |
| title_full | Intermittent hilar occlusion attenuates or prevents renal ischaemia-reperfusion in mice |
| title_fullStr | Intermittent hilar occlusion attenuates or prevents renal ischaemia-reperfusion in mice |
| title_full_unstemmed | Intermittent hilar occlusion attenuates or prevents renal ischaemia-reperfusion in mice |
| title_short | Intermittent hilar occlusion attenuates or prevents renal ischaemia-reperfusion in mice |
| title_sort | intermittent hilar occlusion attenuates or prevents renal ischaemia reperfusion in mice |
| topic | Partial nephrectomy Ischaemia-reperfusion injury Oxidative damage Laboratory animal models |
| url | http://www.sciencedirect.com/science/article/pii/S0753332222008460 |
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