Evaluation of Antiurolithic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino Rats
Background: In ayurvedic system of medicine a vast number of medicinal plants are reported to possess with antiurolithic activity. Aim: To study the antiurolithic activity of alcoholic extract of roots of Cissampelos pareira (AERCP) in chemicals induced urolithiasis in albino rats. Materials a...
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| Format: | Article |
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JCDR Research and Publications Private Limited
2014-07-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/4528/9604_CE(Ra1)_F(H)_PF1(PAK)_PFA(AK)__PF2(AGAK)_U(P).pdf |
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| author | Suresh Babu Sayana Chitra C Khanwelkar Venkat Rao Nimmagadda Vasant R Chavan Ramesh BH Naveen Kumar S |
| author_facet | Suresh Babu Sayana Chitra C Khanwelkar Venkat Rao Nimmagadda Vasant R Chavan Ramesh BH Naveen Kumar S |
| author_sort | Suresh Babu Sayana |
| collection | DOAJ |
| description | Background: In ayurvedic system of medicine a vast number
of medicinal plants are reported to possess with antiurolithic
activity.
Aim: To study the antiurolithic activity of alcoholic extract of
roots of Cissampelos pareira (AERCP) in chemicals induced
urolithiasis in albino rats.
Materials and Methods: Nine Groups of albino rats (n=6) were
used to evaluate the antiurolithic activity of alcoholic extract of
roots of C.Pareira. Group I received with rat chow diet, Group
II with 2% Ammonium chloride (AC) and 0.75% Ethylene glycol
(EG) Group III with EG plus AC and cystone (5 ml/kg), Groups
IV, V, VI with low (100 mg/kg), medium (200 mg/kg), and high
(400 mg/kg) doses of root extract, Groups VII, VIII, IX with EG
plus AC and low (100 mg/kg), medium (200 mg/kg), and high
(400 mg/kg) doses of root extract respectively for 10 days.
Urolithiasis was induced by supplying drinking water mixed
with 2% Ammonium chloride and 0.75% Ethylene glycol for
10 days. On 11th day three rats from each Group were kept in
one metabolic cage and urine (pooled) collected for 24h was
subjected for estimation of biochemical parameters like urinary
calcium, uric acid and magnesium. Blood was collected on the
same day and analysed for various parameters. Kidneys were
observed for the histopathological changes.
Results: The rats treated with alcoholic extract of roots of C.
pareira at 03 different doses significantly (p≤ 0.05) reduced
urinary calcium, uric acid and increased urinary magnesium
levels, reduced serum calcium, creatinine and increased serum
magnesium. Rats treated with 200 mg/kg and 400 mg/kg doses
revealed less tissue damage and the cytology of the nephrotic
tissue was almost similar to normal control Group I rats.
Conclusion: Results showed that alcoholic extract of roots of
C. pareira has exhibited a significant antiurolithic effect against
urolithiasis in experimental rats. |
| first_indexed | 2024-04-11T17:34:00Z |
| format | Article |
| id | doaj.art-028928fe3679438d8aba1f41bd0b0882 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-04-11T17:34:00Z |
| publishDate | 2014-07-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-028928fe3679438d8aba1f41bd0b08822022-12-22T04:11:44ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2014-07-0187HC01HC0410.7860/JCDR/2014/9604.4528Evaluation of Antiurolithic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino RatsSuresh Babu Sayana0Chitra C Khanwelkar1Venkat Rao Nimmagadda2Vasant R Chavan3Ramesh BH4Naveen Kumar S5Ph.D Scholar, Department of Pharmacology, KIMS University, Karad, Maharashtra, India.Professor and HOD, Department of Pharmacology, KIMS University, Karad, Maharashtra, India.Professor and HOD, Department of Pharmacology, VL College of Pharmacy, Raichur, Karnataka, India.Professor and HOD, Department of Pharmacology, RIMS, Raichur, Karnataka, India.Professor and HOD, Department of Pathology, RIMS, Raichur, Karnataka, India.Tutor, Department of Biochemistry, RIMS, Raichur, Karnataka, India.Background: In ayurvedic system of medicine a vast number of medicinal plants are reported to possess with antiurolithic activity. Aim: To study the antiurolithic activity of alcoholic extract of roots of Cissampelos pareira (AERCP) in chemicals induced urolithiasis in albino rats. Materials and Methods: Nine Groups of albino rats (n=6) were used to evaluate the antiurolithic activity of alcoholic extract of roots of C.Pareira. Group I received with rat chow diet, Group II with 2% Ammonium chloride (AC) and 0.75% Ethylene glycol (EG) Group III with EG plus AC and cystone (5 ml/kg), Groups IV, V, VI with low (100 mg/kg), medium (200 mg/kg), and high (400 mg/kg) doses of root extract, Groups VII, VIII, IX with EG plus AC and low (100 mg/kg), medium (200 mg/kg), and high (400 mg/kg) doses of root extract respectively for 10 days. Urolithiasis was induced by supplying drinking water mixed with 2% Ammonium chloride and 0.75% Ethylene glycol for 10 days. On 11th day three rats from each Group were kept in one metabolic cage and urine (pooled) collected for 24h was subjected for estimation of biochemical parameters like urinary calcium, uric acid and magnesium. Blood was collected on the same day and analysed for various parameters. Kidneys were observed for the histopathological changes. Results: The rats treated with alcoholic extract of roots of C. pareira at 03 different doses significantly (p≤ 0.05) reduced urinary calcium, uric acid and increased urinary magnesium levels, reduced serum calcium, creatinine and increased serum magnesium. Rats treated with 200 mg/kg and 400 mg/kg doses revealed less tissue damage and the cytology of the nephrotic tissue was almost similar to normal control Group I rats. Conclusion: Results showed that alcoholic extract of roots of C. pareira has exhibited a significant antiurolithic effect against urolithiasis in experimental rats.https://jcdr.net/articles/PDF/4528/9604_CE(Ra1)_F(H)_PF1(PAK)_PFA(AK)__PF2(AGAK)_U(P).pdfammonium chlorideethylene glycolurolithiasis |
| spellingShingle | Suresh Babu Sayana Chitra C Khanwelkar Venkat Rao Nimmagadda Vasant R Chavan Ramesh BH Naveen Kumar S Evaluation of Antiurolithic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino Rats Journal of Clinical and Diagnostic Research ammonium chloride ethylene glycol urolithiasis |
| title | Evaluation of Antiurolithic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino Rats |
| title_full | Evaluation of Antiurolithic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino Rats |
| title_fullStr | Evaluation of Antiurolithic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino Rats |
| title_full_unstemmed | Evaluation of Antiurolithic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino Rats |
| title_short | Evaluation of Antiurolithic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino Rats |
| title_sort | evaluation of antiurolithic activity of alcoholic extract of roots of cissampelos pareira in albino rats |
| topic | ammonium chloride ethylene glycol urolithiasis |
| url | https://jcdr.net/articles/PDF/4528/9604_CE(Ra1)_F(H)_PF1(PAK)_PFA(AK)__PF2(AGAK)_U(P).pdf |
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