Obesity suppresses circulating level and function of endothelial progenitor cells and heart function
<p>Abstract</p> <p>Background and aim</p> <p>This study tested the hypothesis that obesity suppresses circulating number as well as the function of endothelial progenitor cells (EPCs) and left ventricular ejection fraction (LVEF).</p> <p>Methods</p> &l...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-07-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | http://www.translational-medicine.com/content/10/1/137 |
| _version_ | 1828367102237474816 |
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| author | Tsai Tzu-Hsien Chai Han-Tan Sun Cheuk-Kwan Yen Chia-Hung Leu Steve Chen Yung-Lung Chung Sheng-Ying Ko Sheung-Fat Chang Hsueh-Wen Wu Chiung-Jen Yip Hon-Kan |
| author_facet | Tsai Tzu-Hsien Chai Han-Tan Sun Cheuk-Kwan Yen Chia-Hung Leu Steve Chen Yung-Lung Chung Sheng-Ying Ko Sheung-Fat Chang Hsueh-Wen Wu Chiung-Jen Yip Hon-Kan |
| author_sort | Tsai Tzu-Hsien |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background and aim</p> <p>This study tested the hypothesis that obesity suppresses circulating number as well as the function of endothelial progenitor cells (EPCs) and left ventricular ejection fraction (LVEF).</p> <p>Methods</p> <p>High fat diet (45 Kcal% fat) was given to 8-week-old C57BL/6 J mice (n = 8) for 20 weeks to induce obesity (group 1). Another age-matched group (n = 8) were fed with control diet for 20 weeks as controls (group 2). The animals were sacrificed at the end of 20 weeks after obesity induction.</p> <p>Results</p> <p>By the end of study period, the heart weight, body weight, abdominal fat weight, serum levels of total cholesterol and fasting blood sugar were remarkably higher in group 1 than in group 2 (all p<0.01). The circulating level of EPCs (C-kit/CD31, Sca-1/KDR, CXCR4/CD34) was significantly lower in group 1 than in group 2 (p<0.03) at 18 h after critical limb ischemia induction. The angiogenesis and migratory ability of bone marrow-derived EPCs was remarkably impaired in group 1 compared to that in group 2 (all p<0.01). The repair ability of aortic endothelium damage by lipopolysaccharide was notably attenuated in group 1 compared with that in group 2 (p<0.01). Collagen deposition (Sirius red staining) and fibrotic area (Masson's Trichrome staining) in LV myocardium were notably increased in group 1 compared with group 2 (p<0.001). LVEF was notably lower, whereas LV end-diastolic and end-systolic dimensions were remarkably higher in group 1 than in group 2 (all p<0.001).</p> <p>Conclusions</p> <p>Obesity diminished circulating EPC level, impaired the recovery of damaged endothelium, suppressed EPC angiogenesis ability and LVEF, and increased LV remodeling.</p> |
| first_indexed | 2024-04-14T05:54:50Z |
| format | Article |
| id | doaj.art-02893f371fc248888f6870538dfdbff0 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-04-14T05:54:50Z |
| publishDate | 2012-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-02893f371fc248888f6870538dfdbff02022-12-22T02:08:59ZengBMCJournal of Translational Medicine1479-58762012-07-0110113710.1186/1479-5876-10-137Obesity suppresses circulating level and function of endothelial progenitor cells and heart functionTsai Tzu-HsienChai Han-TanSun Cheuk-KwanYen Chia-HungLeu SteveChen Yung-LungChung Sheng-YingKo Sheung-FatChang Hsueh-WenWu Chiung-JenYip Hon-Kan<p>Abstract</p> <p>Background and aim</p> <p>This study tested the hypothesis that obesity suppresses circulating number as well as the function of endothelial progenitor cells (EPCs) and left ventricular ejection fraction (LVEF).</p> <p>Methods</p> <p>High fat diet (45 Kcal% fat) was given to 8-week-old C57BL/6 J mice (n = 8) for 20 weeks to induce obesity (group 1). Another age-matched group (n = 8) were fed with control diet for 20 weeks as controls (group 2). The animals were sacrificed at the end of 20 weeks after obesity induction.</p> <p>Results</p> <p>By the end of study period, the heart weight, body weight, abdominal fat weight, serum levels of total cholesterol and fasting blood sugar were remarkably higher in group 1 than in group 2 (all p<0.01). The circulating level of EPCs (C-kit/CD31, Sca-1/KDR, CXCR4/CD34) was significantly lower in group 1 than in group 2 (p<0.03) at 18 h after critical limb ischemia induction. The angiogenesis and migratory ability of bone marrow-derived EPCs was remarkably impaired in group 1 compared to that in group 2 (all p<0.01). The repair ability of aortic endothelium damage by lipopolysaccharide was notably attenuated in group 1 compared with that in group 2 (p<0.01). Collagen deposition (Sirius red staining) and fibrotic area (Masson's Trichrome staining) in LV myocardium were notably increased in group 1 compared with group 2 (p<0.001). LVEF was notably lower, whereas LV end-diastolic and end-systolic dimensions were remarkably higher in group 1 than in group 2 (all p<0.001).</p> <p>Conclusions</p> <p>Obesity diminished circulating EPC level, impaired the recovery of damaged endothelium, suppressed EPC angiogenesis ability and LVEF, and increased LV remodeling.</p>http://www.translational-medicine.com/content/10/1/137ObesityAortic endotheliumEndothelial progenitor cellsAngiogenesisLeft ventricular remodeling |
| spellingShingle | Tsai Tzu-Hsien Chai Han-Tan Sun Cheuk-Kwan Yen Chia-Hung Leu Steve Chen Yung-Lung Chung Sheng-Ying Ko Sheung-Fat Chang Hsueh-Wen Wu Chiung-Jen Yip Hon-Kan Obesity suppresses circulating level and function of endothelial progenitor cells and heart function Journal of Translational Medicine Obesity Aortic endothelium Endothelial progenitor cells Angiogenesis Left ventricular remodeling |
| title | Obesity suppresses circulating level and function of endothelial progenitor cells and heart function |
| title_full | Obesity suppresses circulating level and function of endothelial progenitor cells and heart function |
| title_fullStr | Obesity suppresses circulating level and function of endothelial progenitor cells and heart function |
| title_full_unstemmed | Obesity suppresses circulating level and function of endothelial progenitor cells and heart function |
| title_short | Obesity suppresses circulating level and function of endothelial progenitor cells and heart function |
| title_sort | obesity suppresses circulating level and function of endothelial progenitor cells and heart function |
| topic | Obesity Aortic endothelium Endothelial progenitor cells Angiogenesis Left ventricular remodeling |
| url | http://www.translational-medicine.com/content/10/1/137 |
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