Principles and Applications of CRISPR Toolkit in Virus Manipulation, Diagnosis, and Virus-Host Interactions
Viruses are one of the most important concerns for human health, and overcoming viral infections is a worldwide challenge. However, researchers have been trying to manipulate viral genomes to overcome various disorders, including cancer, for vaccine development purposes. CRISPR (clustered regularly...
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| Format: | Article |
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MDPI AG
2022-03-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/6/999 |
| _version_ | 1797472344635080704 |
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| author | Saleh Jamehdor Sara Pajouhanfar Sadaf Saba Georges Uzan Ali Teimoori Sina Naserian |
| author_facet | Saleh Jamehdor Sara Pajouhanfar Sadaf Saba Georges Uzan Ali Teimoori Sina Naserian |
| author_sort | Saleh Jamehdor |
| collection | DOAJ |
| description | Viruses are one of the most important concerns for human health, and overcoming viral infections is a worldwide challenge. However, researchers have been trying to manipulate viral genomes to overcome various disorders, including cancer, for vaccine development purposes. CRISPR (clustered regularly interspaced short palindromic repeats) is becoming one of the most functional and widely used tools for RNA and DNA manipulation in multiple organisms. This approach has provided an unprecedented opportunity for creating simple, inexpensive, specific, targeted, accurate, and practical manipulations of viruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), human immunodeficiency virus-1 (HIV-1), and vaccinia virus. Furthermore, this method can be used to make an effective and precise diagnosis of viral infections. Nevertheless, a valid and scientifically designed CRISPR system is critical to make more effective and accurate changes in viruses. In this review, we have focused on the best and the most effective ways to design sgRNA, gene knock-in(s), and gene knock-out(s) for virus-targeted manipulation. Furthermore, we have emphasized the application of CRISPR technology in virus diagnosis and in finding significant genes involved in virus-host interactions. |
| first_indexed | 2024-03-09T20:00:33Z |
| format | Article |
| id | doaj.art-0289c823ad8f4e7ebe511672a6e31d2f |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T20:00:33Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-0289c823ad8f4e7ebe511672a6e31d2f2023-11-24T00:44:25ZengMDPI AGCells2073-44092022-03-0111699910.3390/cells11060999Principles and Applications of CRISPR Toolkit in Virus Manipulation, Diagnosis, and Virus-Host InteractionsSaleh Jamehdor0Sara Pajouhanfar1Sadaf Saba2Georges Uzan3Ali Teimoori4Sina Naserian5Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan 989155432609, IranDepartment of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110, USACenter for Molecular Medicine & Genetics, Wayne State University School of Medicine, Detroit, MI 48201, USAINSERM UMR-S-MD 1197, Hôpital Paul Brousse, 94800 Villejuif, FranceDepartment of Virology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan 6517838738, IranINSERM UMR-S-MD 1197, Hôpital Paul Brousse, 94800 Villejuif, FranceViruses are one of the most important concerns for human health, and overcoming viral infections is a worldwide challenge. However, researchers have been trying to manipulate viral genomes to overcome various disorders, including cancer, for vaccine development purposes. CRISPR (clustered regularly interspaced short palindromic repeats) is becoming one of the most functional and widely used tools for RNA and DNA manipulation in multiple organisms. This approach has provided an unprecedented opportunity for creating simple, inexpensive, specific, targeted, accurate, and practical manipulations of viruses, such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), human immunodeficiency virus-1 (HIV-1), and vaccinia virus. Furthermore, this method can be used to make an effective and precise diagnosis of viral infections. Nevertheless, a valid and scientifically designed CRISPR system is critical to make more effective and accurate changes in viruses. In this review, we have focused on the best and the most effective ways to design sgRNA, gene knock-in(s), and gene knock-out(s) for virus-targeted manipulation. Furthermore, we have emphasized the application of CRISPR technology in virus diagnosis and in finding significant genes involved in virus-host interactions.https://www.mdpi.com/2073-4409/11/6/999virusCRISPRgenetic engineeringSARS-CoV-2HIV-1 |
| spellingShingle | Saleh Jamehdor Sara Pajouhanfar Sadaf Saba Georges Uzan Ali Teimoori Sina Naserian Principles and Applications of CRISPR Toolkit in Virus Manipulation, Diagnosis, and Virus-Host Interactions Cells virus CRISPR genetic engineering SARS-CoV-2 HIV-1 |
| title | Principles and Applications of CRISPR Toolkit in Virus Manipulation, Diagnosis, and Virus-Host Interactions |
| title_full | Principles and Applications of CRISPR Toolkit in Virus Manipulation, Diagnosis, and Virus-Host Interactions |
| title_fullStr | Principles and Applications of CRISPR Toolkit in Virus Manipulation, Diagnosis, and Virus-Host Interactions |
| title_full_unstemmed | Principles and Applications of CRISPR Toolkit in Virus Manipulation, Diagnosis, and Virus-Host Interactions |
| title_short | Principles and Applications of CRISPR Toolkit in Virus Manipulation, Diagnosis, and Virus-Host Interactions |
| title_sort | principles and applications of crispr toolkit in virus manipulation diagnosis and virus host interactions |
| topic | virus CRISPR genetic engineering SARS-CoV-2 HIV-1 |
| url | https://www.mdpi.com/2073-4409/11/6/999 |
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