Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells
Introduction: Delivery of therapeutic nanoparticles (NPs) to cancer cells represents a promising approach for biomedical applications. A key challenge for nanotechnology translation from the bench to the bedside is the low amount of administered NPs dose that effectively enters target cells. To impr...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-07-01
|
| Series: | Frontiers in Nanotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnano.2023.1220514/full |
| _version_ | 1797778008107384832 |
|---|---|
| author | Eva Susnik Amelie Bazzoni Patricia Taladriz-Blanco Sandor Balog Aura Maria Moreno-Echeverri Christina Glaubitz Beatriz Brito Oliveira Daniela Ferreira Pedro Viana Baptista Alke Petri-Fink Alke Petri-Fink Barbara Rothen-Rutishauser |
| author_facet | Eva Susnik Amelie Bazzoni Patricia Taladriz-Blanco Sandor Balog Aura Maria Moreno-Echeverri Christina Glaubitz Beatriz Brito Oliveira Daniela Ferreira Pedro Viana Baptista Alke Petri-Fink Alke Petri-Fink Barbara Rothen-Rutishauser |
| author_sort | Eva Susnik |
| collection | DOAJ |
| description | Introduction: Delivery of therapeutic nanoparticles (NPs) to cancer cells represents a promising approach for biomedical applications. A key challenge for nanotechnology translation from the bench to the bedside is the low amount of administered NPs dose that effectively enters target cells. To improve NPs delivery, several studies proposed NPs conjugation with ligands, which specifically deliver NPs to target cells via receptor binding. One such example is epidermal growth factor (EGF), a peptide involved in cell signaling pathways that control cell division by binding to epidermal growth factor receptor (EGFR). However, very few studies assessed the influence of EGF present in the cell environment, on the cellular uptake of NPs.Methods: We tested if the stimulation of EGFR-expressing lung carcinomacells A549 with EGF affects the uptake of 59 nm and 422 nm silica (SiO2) NPs. Additionally, we investigated whether the uptake enhancement can be achieved with gold NPs, suitable to downregulate the expression of cancer oncogene c-MYC.Results: Our findings show that EGF binding to its receptor results in receptor autophosphorylation and initiate signaling pathways, leading to enhanced endocytosis of 59 nm SiO2 NPs, but not 422 nm SiO2 NPs. Additionally, we demonstrated an enhanced gold (Au) NPs endocytosis and subsequently a higher downregulation of c-MYC.Discussion: These findings contribute to a better understanding of NPs uptake in the presence of EGF and that is a promising approach for improved NPs delivery. |
| first_indexed | 2024-03-12T23:11:09Z |
| format | Article |
| id | doaj.art-028e2419615345e8b751c72a395a7988 |
| institution | Directory Open Access Journal |
| issn | 2673-3013 |
| language | English |
| last_indexed | 2024-03-12T23:11:09Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nanotechnology |
| spelling | doaj.art-028e2419615345e8b751c72a395a79882023-07-18T00:58:24ZengFrontiers Media S.A.Frontiers in Nanotechnology2673-30132023-07-01510.3389/fnano.2023.12205141220514Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cellsEva Susnik0Amelie Bazzoni1Patricia Taladriz-Blanco2Sandor Balog3Aura Maria Moreno-Echeverri4Christina Glaubitz5Beatriz Brito Oliveira6Daniela Ferreira7Pedro Viana Baptista8Alke Petri-Fink9Alke Petri-Fink10Barbara Rothen-Rutishauser11Adolphe Merkle Institute, University of Fribourg, Fribourg, SwitzerlandAdolphe Merkle Institute, University of Fribourg, Fribourg, SwitzerlandInternational Iberian Nanotechnology Laboratory, Braga, PortugalAdolphe Merkle Institute, University of Fribourg, Fribourg, SwitzerlandAdolphe Merkle Institute, University of Fribourg, Fribourg, SwitzerlandAdolphe Merkle Institute, University of Fribourg, Fribourg, Switzerlandi4HB, UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Caparica, Portugali4HB, UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Caparica, Portugali4HB, UCIBIO, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Caparica, PortugalAdolphe Merkle Institute, University of Fribourg, Fribourg, SwitzerlandDepartment of Chemistry, University of Fribourg, Fribourg, SwitzerlandAdolphe Merkle Institute, University of Fribourg, Fribourg, SwitzerlandIntroduction: Delivery of therapeutic nanoparticles (NPs) to cancer cells represents a promising approach for biomedical applications. A key challenge for nanotechnology translation from the bench to the bedside is the low amount of administered NPs dose that effectively enters target cells. To improve NPs delivery, several studies proposed NPs conjugation with ligands, which specifically deliver NPs to target cells via receptor binding. One such example is epidermal growth factor (EGF), a peptide involved in cell signaling pathways that control cell division by binding to epidermal growth factor receptor (EGFR). However, very few studies assessed the influence of EGF present in the cell environment, on the cellular uptake of NPs.Methods: We tested if the stimulation of EGFR-expressing lung carcinomacells A549 with EGF affects the uptake of 59 nm and 422 nm silica (SiO2) NPs. Additionally, we investigated whether the uptake enhancement can be achieved with gold NPs, suitable to downregulate the expression of cancer oncogene c-MYC.Results: Our findings show that EGF binding to its receptor results in receptor autophosphorylation and initiate signaling pathways, leading to enhanced endocytosis of 59 nm SiO2 NPs, but not 422 nm SiO2 NPs. Additionally, we demonstrated an enhanced gold (Au) NPs endocytosis and subsequently a higher downregulation of c-MYC.Discussion: These findings contribute to a better understanding of NPs uptake in the presence of EGF and that is a promising approach for improved NPs delivery.https://www.frontiersin.org/articles/10.3389/fnano.2023.1220514/fullepidermal growth factornanoparticlessilicagoldendocytosismolecular mechanisms |
| spellingShingle | Eva Susnik Amelie Bazzoni Patricia Taladriz-Blanco Sandor Balog Aura Maria Moreno-Echeverri Christina Glaubitz Beatriz Brito Oliveira Daniela Ferreira Pedro Viana Baptista Alke Petri-Fink Alke Petri-Fink Barbara Rothen-Rutishauser Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells Frontiers in Nanotechnology epidermal growth factor nanoparticles silica gold endocytosis molecular mechanisms |
| title | Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells |
| title_full | Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells |
| title_fullStr | Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells |
| title_full_unstemmed | Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells |
| title_short | Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells |
| title_sort | epidermal growth factor alters silica nanoparticle uptake and improves gold nanoparticle mediated gene silencing in a549 cells |
| topic | epidermal growth factor nanoparticles silica gold endocytosis molecular mechanisms |
| url | https://www.frontiersin.org/articles/10.3389/fnano.2023.1220514/full |
| work_keys_str_mv | AT evasusnik epidermalgrowthfactoralterssilicananoparticleuptakeandimprovesgoldnanoparticlemediatedgenesilencingina549cells AT ameliebazzoni epidermalgrowthfactoralterssilicananoparticleuptakeandimprovesgoldnanoparticlemediatedgenesilencingina549cells AT patriciataladrizblanco epidermalgrowthfactoralterssilicananoparticleuptakeandimprovesgoldnanoparticlemediatedgenesilencingina549cells AT sandorbalog epidermalgrowthfactoralterssilicananoparticleuptakeandimprovesgoldnanoparticlemediatedgenesilencingina549cells AT auramariamorenoecheverri epidermalgrowthfactoralterssilicananoparticleuptakeandimprovesgoldnanoparticlemediatedgenesilencingina549cells AT christinaglaubitz epidermalgrowthfactoralterssilicananoparticleuptakeandimprovesgoldnanoparticlemediatedgenesilencingina549cells AT beatrizbritooliveira epidermalgrowthfactoralterssilicananoparticleuptakeandimprovesgoldnanoparticlemediatedgenesilencingina549cells AT danielaferreira epidermalgrowthfactoralterssilicananoparticleuptakeandimprovesgoldnanoparticlemediatedgenesilencingina549cells AT pedrovianabaptista epidermalgrowthfactoralterssilicananoparticleuptakeandimprovesgoldnanoparticlemediatedgenesilencingina549cells AT alkepetrifink epidermalgrowthfactoralterssilicananoparticleuptakeandimprovesgoldnanoparticlemediatedgenesilencingina549cells AT alkepetrifink epidermalgrowthfactoralterssilicananoparticleuptakeandimprovesgoldnanoparticlemediatedgenesilencingina549cells AT barbararothenrutishauser epidermalgrowthfactoralterssilicananoparticleuptakeandimprovesgoldnanoparticlemediatedgenesilencingina549cells |