Ethanolic extract of Aloe vera ameliorates sciatic nerve ligation induced neuropathic pain
Background: Aloe vera is being used since ages by human kind for treating various ailments including various inflammatory conditions, but scientific validation has not been done for analgesic activity against neuropathic pain. Objective: The current study was designed to systematically evaluate the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | Ancient Science of Life |
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| Online Access: | http://www.ancientscienceoflife.org/article.asp?issn=0257-7941;year=2014;volume=33;issue=4;spage=208;epage=215;aulast=Kanyadhara |
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| author | Swetha Kanyadhara Sujatha Dodoala Sunitha Sampathi Priyanka Punuru Gopichand Chinta |
| author_facet | Swetha Kanyadhara Sujatha Dodoala Sunitha Sampathi Priyanka Punuru Gopichand Chinta |
| author_sort | Swetha Kanyadhara |
| collection | DOAJ |
| description | Background: Aloe vera is being used since ages by human kind for treating various ailments including various inflammatory conditions, but scientific validation has not been done for analgesic activity against neuropathic pain.
Objective: The current study was designed to systematically evaluate the therapeutic potential of the ethanolic extract of A. vera (EEAV) against sciatic nerve ligation (SCNL) induced neuropathic pain.
Materials and Methods: Nociceptive threshold of EEAV against thermal hyperalgesia, chemical hyperalgesia and mechanical allodynia were performed on 0, 7, 14 and 21 st day post-SCNL. Serum total protein, serum nitrite, in vivo anti-oxidant parameters and lipid peroxidation (LPO) were estimated. Sciatic nerve homogenate was used to estimate myeloperoxidase (MPO) and calcium levels. Histopathology of the sciatic nerve was done to confirm the biochemical findings.
Results: Treatment with ethanolic extract has increased the threshold for the nociception in thermal hyperalgesia, chemical hyperalgesia and mechanical allodynia models. A significant improvement of in vivo anti-oxidant parameters and decreased LPO levels were observed on treatment with A. vera. Significant decrease in serum nitrite, protein, calcium and MPO levels were observed, indicating protection against damage caused by SCNL.
Conclusion: The results of the present study validate the use of EEAV to treat neuropathic pain. This effect may be attributed to the decreased migration of neutrophils and due to the anti-oxidant properties of A. vera. Further studies to confirm the mechanism of action will help develop suitable A. vera formulations for neuropathic pain therapy . |
| first_indexed | 2024-12-20T09:38:46Z |
| format | Article |
| id | doaj.art-028e7c0f58384b2792f0dd32420e8021 |
| institution | Directory Open Access Journal |
| issn | 0257-7941 |
| language | English |
| last_indexed | 2024-12-20T09:38:46Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Ancient Science of Life |
| spelling | doaj.art-028e7c0f58384b2792f0dd32420e80212022-12-21T19:44:55ZengWolters Kluwer Medknow PublicationsAncient Science of Life0257-79412014-01-0133420821510.4103/0257-7941.147425Ethanolic extract of Aloe vera ameliorates sciatic nerve ligation induced neuropathic painSwetha KanyadharaSujatha DodoalaSunitha SampathiPriyanka PunuruGopichand ChintaBackground: Aloe vera is being used since ages by human kind for treating various ailments including various inflammatory conditions, but scientific validation has not been done for analgesic activity against neuropathic pain. Objective: The current study was designed to systematically evaluate the therapeutic potential of the ethanolic extract of A. vera (EEAV) against sciatic nerve ligation (SCNL) induced neuropathic pain. Materials and Methods: Nociceptive threshold of EEAV against thermal hyperalgesia, chemical hyperalgesia and mechanical allodynia were performed on 0, 7, 14 and 21 st day post-SCNL. Serum total protein, serum nitrite, in vivo anti-oxidant parameters and lipid peroxidation (LPO) were estimated. Sciatic nerve homogenate was used to estimate myeloperoxidase (MPO) and calcium levels. Histopathology of the sciatic nerve was done to confirm the biochemical findings. Results: Treatment with ethanolic extract has increased the threshold for the nociception in thermal hyperalgesia, chemical hyperalgesia and mechanical allodynia models. A significant improvement of in vivo anti-oxidant parameters and decreased LPO levels were observed on treatment with A. vera. Significant decrease in serum nitrite, protein, calcium and MPO levels were observed, indicating protection against damage caused by SCNL. Conclusion: The results of the present study validate the use of EEAV to treat neuropathic pain. This effect may be attributed to the decreased migration of neutrophils and due to the anti-oxidant properties of A. vera. Further studies to confirm the mechanism of action will help develop suitable A. vera formulations for neuropathic pain therapy .http://www.ancientscienceoflife.org/article.asp?issn=0257-7941;year=2014;volume=33;issue=4;spage=208;epage=215;aulast=KanyadharaAloe verachronic constriction injuryneuropathic painsciatic nerve ligation |
| spellingShingle | Swetha Kanyadhara Sujatha Dodoala Sunitha Sampathi Priyanka Punuru Gopichand Chinta Ethanolic extract of Aloe vera ameliorates sciatic nerve ligation induced neuropathic pain Ancient Science of Life Aloe vera chronic constriction injury neuropathic pain sciatic nerve ligation |
| title | Ethanolic extract of Aloe vera ameliorates sciatic nerve ligation induced neuropathic pain |
| title_full | Ethanolic extract of Aloe vera ameliorates sciatic nerve ligation induced neuropathic pain |
| title_fullStr | Ethanolic extract of Aloe vera ameliorates sciatic nerve ligation induced neuropathic pain |
| title_full_unstemmed | Ethanolic extract of Aloe vera ameliorates sciatic nerve ligation induced neuropathic pain |
| title_short | Ethanolic extract of Aloe vera ameliorates sciatic nerve ligation induced neuropathic pain |
| title_sort | ethanolic extract of aloe vera ameliorates sciatic nerve ligation induced neuropathic pain |
| topic | Aloe vera chronic constriction injury neuropathic pain sciatic nerve ligation |
| url | http://www.ancientscienceoflife.org/article.asp?issn=0257-7941;year=2014;volume=33;issue=4;spage=208;epage=215;aulast=Kanyadhara |
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