Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, Antarctica
The permanently ice-covered lakes of the McMurdo Dry Valleys, Antarctica are distinctive ecosystems that consist strictly of microbial communities. In this study, water samples were collected from Lake Vanda, a stratified Dry Valley lake whose upper waters (from just below the ice cover to nearly 60...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-07-01
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| Series: | Microorganisms |
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| Online Access: | http://www.mdpi.com/2076-2607/3/3/391 |
| _version_ | 1818959178083860480 |
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| author | Nicole A. Vander Schaaf Anna M. G. Cunningham Brandon P. Cluff CodyJo K. Kraemer Chelsea L. Reeves Carli J. Riester Lauren K. Slater Michael T. Madigan W. Matthew Sattley |
| author_facet | Nicole A. Vander Schaaf Anna M. G. Cunningham Brandon P. Cluff CodyJo K. Kraemer Chelsea L. Reeves Carli J. Riester Lauren K. Slater Michael T. Madigan W. Matthew Sattley |
| author_sort | Nicole A. Vander Schaaf |
| collection | DOAJ |
| description | The permanently ice-covered lakes of the McMurdo Dry Valleys, Antarctica are distinctive ecosystems that consist strictly of microbial communities. In this study, water samples were collected from Lake Vanda, a stratified Dry Valley lake whose upper waters (from just below the ice cover to nearly 60 m) are highly oligotrophic, and used to establish enrichment cultures. Six strains of psychrotolerant, heterotrophic bacteria were isolated from lake water samples from a depth of 50 or 55 m. Phylogenetic analyses showed the Lake Vanda strains to be species of Nocardiaceae, Caulobacteraceae, Sphingomonadaceae, and Bradyrhizobiaceae. All Lake Vanda strains grew at temperatures near or below 0 °C, but optimal growth occurred from 18 to 24 °C. Some strains showed significant halotolerance, but no strains required NaCl for growth. The isolates described herein include cold-active species not previously reported from Dry Valley lakes, and their physiological and phylogenetic characterization broadens our understanding of these limnologically unique lakes. |
| first_indexed | 2024-12-20T11:37:30Z |
| format | Article |
| id | doaj.art-028f9fb4d5ff4d709f5a5036c68ff525 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-20T11:37:30Z |
| publishDate | 2015-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-028f9fb4d5ff4d709f5a5036c68ff5252022-12-21T19:42:04ZengMDPI AGMicroorganisms2076-26072015-07-013339140610.3390/microorganisms3030391microorganisms3030391Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, AntarcticaNicole A. Vander Schaaf0Anna M. G. Cunningham1Brandon P. Cluff2CodyJo K. Kraemer3Chelsea L. Reeves4Carli J. Riester5Lauren K. Slater6Michael T. Madigan7W. Matthew Sattley8Division of Natural Sciences, Indiana Wesleyan University, 4201 South Washington St., Marion, IN 46953-4974, USADivision of Natural Sciences, Indiana Wesleyan University, 4201 South Washington St., Marion, IN 46953-4974, USADepartment of Biology, MidAmerica Nazarene University, 2030 E. College Way, Olathe, KS 66062, USADepartment of Biology, MidAmerica Nazarene University, 2030 E. College Way, Olathe, KS 66062, USADivision of Natural Sciences, Indiana Wesleyan University, 4201 South Washington St., Marion, IN 46953-4974, USADivision of Natural Sciences, Indiana Wesleyan University, 4201 South Washington St., Marion, IN 46953-4974, USADivision of Natural Sciences, Indiana Wesleyan University, 4201 South Washington St., Marion, IN 46953-4974, USADepartment of Microbiology, Southern Illinois University, 1125 Lincoln Dr., Carbondale, IL 62901, USADivision of Natural Sciences, Indiana Wesleyan University, 4201 South Washington St., Marion, IN 46953-4974, USAThe permanently ice-covered lakes of the McMurdo Dry Valleys, Antarctica are distinctive ecosystems that consist strictly of microbial communities. In this study, water samples were collected from Lake Vanda, a stratified Dry Valley lake whose upper waters (from just below the ice cover to nearly 60 m) are highly oligotrophic, and used to establish enrichment cultures. Six strains of psychrotolerant, heterotrophic bacteria were isolated from lake water samples from a depth of 50 or 55 m. Phylogenetic analyses showed the Lake Vanda strains to be species of Nocardiaceae, Caulobacteraceae, Sphingomonadaceae, and Bradyrhizobiaceae. All Lake Vanda strains grew at temperatures near or below 0 °C, but optimal growth occurred from 18 to 24 °C. Some strains showed significant halotolerance, but no strains required NaCl for growth. The isolates described herein include cold-active species not previously reported from Dry Valley lakes, and their physiological and phylogenetic characterization broadens our understanding of these limnologically unique lakes.http://www.mdpi.com/2076-2607/3/3/391AntarcticaLake VandaMcMurdo Dry Valleyspsychrotoleranthalotolerant |
| spellingShingle | Nicole A. Vander Schaaf Anna M. G. Cunningham Brandon P. Cluff CodyJo K. Kraemer Chelsea L. Reeves Carli J. Riester Lauren K. Slater Michael T. Madigan W. Matthew Sattley Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, Antarctica Microorganisms Antarctica Lake Vanda McMurdo Dry Valleys psychrotolerant halotolerant |
| title | Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, Antarctica |
| title_full | Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, Antarctica |
| title_fullStr | Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, Antarctica |
| title_full_unstemmed | Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, Antarctica |
| title_short | Cold-Active, Heterotrophic Bacteria from the Highly Oligotrophic Waters of Lake Vanda, Antarctica |
| title_sort | cold active heterotrophic bacteria from the highly oligotrophic waters of lake vanda antarctica |
| topic | Antarctica Lake Vanda McMurdo Dry Valleys psychrotolerant halotolerant |
| url | http://www.mdpi.com/2076-2607/3/3/391 |
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